Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4417 and Pf6N2E2_4418 are separated by 13 nucleotides Pf6N2E2_4418 and Pf6N2E2_4419 are separated by 4 nucleotides Pf6N2E2_4419 and Pf6N2E2_4420 are separated by 9 nucleotides Pf6N2E2_4420 and Pf6N2E2_4421 overlap by 18 nucleotides Pf6N2E2_4421 and Pf6N2E2_4422 overlap by 4 nucleotides Pf6N2E2_4422 and Pf6N2E2_4423 are separated by 4 nucleotides
Pf6N2E2_4417 - Protein of unknown function DUF484, at 5,150,502 to 5,151,224
_4417
Pf6N2E2_4418 - Diaminopimelate epimerase (EC 5.1.1.7), at 5,151,238 to 5,152,068
_4418
Pf6N2E2_4419 - Diaminopimelate decarboxylase (EC 4.1.1.20), at 5,152,073 to 5,153,320
_4419
Pf6N2E2_4420 - Lipoprotein LppL-related protein, at 5,153,330 to 5,153,452
_4420
Pf6N2E2_4421 - hypothetical protein, at 5,153,435 to 5,153,692
_4421
Pf6N2E2_4422 - Frataxin homolog CyaY, facilitates iron supply for heme A synthesis or Fe-S cluster assembly, at 5,153,689 to 5,154,021
_4422
Pf6N2E2_4423 - FIG00953342: hypothetical protein, at 5,154,026 to 5,154,244
_4423
Position (kb)
5152
5153
5154 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5151.261 kb on - strand at 5151.262 kb on + strand at 5151.263 kb on - strand at 5151.263 kb on - strand at 5151.907 kb on - strand, within Pf6N2E2_4418 at 5152.054 kb on - strand at 5153.332 kb on - strand at 5153.335 kb on - strand at 5153.335 kb on - strand at 5153.335 kb on - strand at 5153.335 kb on - strand at 5153.335 kb on - strand at 5153.335 kb on - strand at 5153.335 kb on - strand at 5153.335 kb on - strand at 5153.335 kb on - strand at 5153.335 kb on - strand at 5153.541 kb on - strand, within Pf6N2E2_4421 at 5153.541 kb on - strand, within Pf6N2E2_4421 at 5153.543 kb on - strand, within Pf6N2E2_4421 at 5153.566 kb on - strand, within Pf6N2E2_4421 at 5153.611 kb on + strand, within Pf6N2E2_4421 at 5153.612 kb on - strand, within Pf6N2E2_4421 at 5153.612 kb on - strand, within Pf6N2E2_4421 at 5153.613 kb on + strand, within Pf6N2E2_4421 at 5153.614 kb on - strand, within Pf6N2E2_4421 at 5153.614 kb on - strand, within Pf6N2E2_4421 at 5153.614 kb on - strand, within Pf6N2E2_4421 at 5153.642 kb on + strand, within Pf6N2E2_4421 at 5153.642 kb on + strand, within Pf6N2E2_4421 at 5153.643 kb on - strand, within Pf6N2E2_4421 at 5153.801 kb on + strand, within Pf6N2E2_4422 at 5153.801 kb on + strand, within Pf6N2E2_4422 at 5153.905 kb on + strand, within Pf6N2E2_4422 at 5153.906 kb on - strand, within Pf6N2E2_4422 at 5154.019 kb on + strand at 5154.065 kb on + strand, within Pf6N2E2_4423 at 5154.066 kb on - strand, within Pf6N2E2_4423 at 5154.066 kb on - strand, within Pf6N2E2_4423 at 5154.066 kb on - strand, within Pf6N2E2_4423 at 5154.205 kb on + strand, within Pf6N2E2_4423 at 5154.242 kb on + strand at 5154.242 kb on + strand at 5154.243 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 5,151,261 - -1.2 5,151,262 + -0.3 5,151,263 - +1.5 5,151,263 - -1.5 5,151,907 - Pf6N2E2_4418 0.81 +0.7 5,152,054 - +1.2 5,153,332 - -2.1 5,153,335 - -1.0 5,153,335 - +3.2 5,153,335 - +0.0 5,153,335 - +0.2 5,153,335 - +0.9 5,153,335 - -1.2 5,153,335 - +1.5 5,153,335 - +0.8 5,153,335 - -0.1 5,153,335 - +0.4 5,153,541 - Pf6N2E2_4421 0.41 +1.0 5,153,541 - Pf6N2E2_4421 0.41 -0.4 5,153,543 - Pf6N2E2_4421 0.42 -1.3 5,153,566 - Pf6N2E2_4421 0.51 -0.9 5,153,611 + Pf6N2E2_4421 0.68 +1.2 5,153,612 - Pf6N2E2_4421 0.69 -1.5 5,153,612 - Pf6N2E2_4421 0.69 -0.8 5,153,613 + Pf6N2E2_4421 0.69 -1.0 5,153,614 - Pf6N2E2_4421 0.69 -0.1 5,153,614 - Pf6N2E2_4421 0.69 -0.2 5,153,614 - Pf6N2E2_4421 0.69 -2.6 5,153,642 + Pf6N2E2_4421 0.80 +0.7 5,153,642 + Pf6N2E2_4421 0.80 +0.4 5,153,643 - Pf6N2E2_4421 0.81 -0.0 5,153,801 + Pf6N2E2_4422 0.34 -0.8 5,153,801 + Pf6N2E2_4422 0.34 +1.3 5,153,905 + Pf6N2E2_4422 0.65 -2.9 5,153,906 - Pf6N2E2_4422 0.65 +0.4 5,154,019 + -1.3 5,154,065 + Pf6N2E2_4423 0.18 -2.3 5,154,066 - Pf6N2E2_4423 0.18 +0.2 5,154,066 - Pf6N2E2_4423 0.18 +1.6 5,154,066 - Pf6N2E2_4423 0.18 -0.8 5,154,205 + Pf6N2E2_4423 0.82 -0.9 5,154,242 + +2.5 5,154,242 + -0.2 5,154,243 - -1.4
Or see this region's nucleotide sequence