Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3585 and Pf6N2E2_3586 are separated by 4 nucleotides Pf6N2E2_3586 and Pf6N2E2_3587 are separated by 319 nucleotides Pf6N2E2_3587 and Pf6N2E2_3588 are separated by 143 nucleotides
Pf6N2E2_3585 - Nicotinate phosphoribosyltransferase (EC 2.4.2.11), at 4,176,638 to 4,177,864
_3585
Pf6N2E2_3586 - NAD synthetase (EC 6.3.1.5), at 4,177,869 to 4,178,696
_3586
Pf6N2E2_3587 - Azurin, at 4,179,016 to 4,179,462
_3587
Pf6N2E2_3588 - hypothetical protein, at 4,179,606 to 4,179,743
_3588
Position (kb)
4177
4178
4179 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4176.933 kb on - strand, within Pf6N2E2_3585 at 4176.963 kb on + strand, within Pf6N2E2_3585 at 4176.963 kb on + strand, within Pf6N2E2_3585 at 4176.963 kb on + strand, within Pf6N2E2_3585 at 4176.963 kb on + strand, within Pf6N2E2_3585 at 4176.963 kb on + strand, within Pf6N2E2_3585 at 4176.963 kb on + strand, within Pf6N2E2_3585 at 4176.963 kb on + strand, within Pf6N2E2_3585 at 4176.964 kb on - strand, within Pf6N2E2_3585 at 4176.964 kb on - strand, within Pf6N2E2_3585 at 4177.392 kb on - strand, within Pf6N2E2_3585 at 4177.645 kb on + strand, within Pf6N2E2_3585 at 4177.646 kb on - strand, within Pf6N2E2_3585 at 4177.696 kb on - strand, within Pf6N2E2_3585 at 4177.696 kb on - strand, within Pf6N2E2_3585 at 4177.696 kb on - strand, within Pf6N2E2_3585 at 4177.717 kb on + strand, within Pf6N2E2_3585 at 4177.805 kb on + strand at 4177.805 kb on + strand at 4177.805 kb on + strand at 4177.805 kb on + strand at 4177.806 kb on - strand at 4177.827 kb on + strand at 4177.827 kb on + strand at 4177.828 kb on - strand at 4177.879 kb on + strand at 4177.970 kb on + strand, within Pf6N2E2_3586 at 4177.971 kb on - strand, within Pf6N2E2_3586 at 4178.106 kb on + strand, within Pf6N2E2_3586 at 4178.106 kb on + strand, within Pf6N2E2_3586 at 4178.297 kb on + strand, within Pf6N2E2_3586 at 4178.297 kb on + strand, within Pf6N2E2_3586 at 4178.315 kb on + strand, within Pf6N2E2_3586 at 4178.315 kb on + strand, within Pf6N2E2_3586 at 4178.315 kb on + strand, within Pf6N2E2_3586 at 4178.316 kb on - strand, within Pf6N2E2_3586 at 4178.328 kb on + strand, within Pf6N2E2_3586 at 4178.328 kb on + strand, within Pf6N2E2_3586 at 4178.328 kb on + strand, within Pf6N2E2_3586 at 4178.328 kb on + strand, within Pf6N2E2_3586 at 4178.328 kb on + strand, within Pf6N2E2_3586 at 4178.328 kb on + strand, within Pf6N2E2_3586 at 4178.329 kb on - strand, within Pf6N2E2_3586 at 4178.329 kb on - strand, within Pf6N2E2_3586 at 4178.329 kb on - strand, within Pf6N2E2_3586 at 4178.580 kb on + strand, within Pf6N2E2_3586 at 4178.580 kb on + strand, within Pf6N2E2_3586 at 4178.580 kb on + strand, within Pf6N2E2_3586 at 4178.581 kb on - strand, within Pf6N2E2_3586 at 4178.652 kb on + strand at 4178.652 kb on + strand at 4178.699 kb on - strand at 4178.799 kb on + strand at 4178.981 kb on - strand at 4179.080 kb on + strand, within Pf6N2E2_3587 at 4179.081 kb on - strand, within Pf6N2E2_3587 at 4179.188 kb on + strand, within Pf6N2E2_3587 at 4179.188 kb on + strand, within Pf6N2E2_3587 at 4179.188 kb on + strand, within Pf6N2E2_3587 at 4179.188 kb on + strand, within Pf6N2E2_3587 at 4179.189 kb on - strand, within Pf6N2E2_3587 at 4179.189 kb on - strand, within Pf6N2E2_3587 at 4179.440 kb on - strand at 4179.534 kb on + strand at 4179.564 kb on + strand at 4179.564 kb on + strand at 4179.571 kb on - strand at 4179.571 kb on - strand at 4179.618 kb on + strand at 4179.619 kb on - strand at 4179.619 kb on - strand at 4179.619 kb on - strand at 4179.619 kb on - strand at 4179.673 kb on + strand, within Pf6N2E2_3588 at 4179.673 kb on + strand, within Pf6N2E2_3588 at 4179.674 kb on - strand, within Pf6N2E2_3588
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 4,176,933 - Pf6N2E2_3585 0.24 +3.2 4,176,963 + Pf6N2E2_3585 0.26 +0.4 4,176,963 + Pf6N2E2_3585 0.26 +1.1 4,176,963 + Pf6N2E2_3585 0.26 +0.5 4,176,963 + Pf6N2E2_3585 0.26 -0.4 4,176,963 + Pf6N2E2_3585 0.26 +0.4 4,176,963 + Pf6N2E2_3585 0.26 -0.5 4,176,963 + Pf6N2E2_3585 0.26 -0.5 4,176,964 - Pf6N2E2_3585 0.27 -0.6 4,176,964 - Pf6N2E2_3585 0.27 -0.6 4,177,392 - Pf6N2E2_3585 0.61 +1.1 4,177,645 + Pf6N2E2_3585 0.82 -0.3 4,177,646 - Pf6N2E2_3585 0.82 -0.8 4,177,696 - Pf6N2E2_3585 0.86 -0.7 4,177,696 - Pf6N2E2_3585 0.86 -0.3 4,177,696 - Pf6N2E2_3585 0.86 +0.0 4,177,717 + Pf6N2E2_3585 0.88 +0.3 4,177,805 + +0.6 4,177,805 + +1.7 4,177,805 + +0.2 4,177,805 + -1.0 4,177,806 - -0.4 4,177,827 + +0.8 4,177,827 + -1.0 4,177,828 - -2.6 4,177,879 + +0.3 4,177,970 + Pf6N2E2_3586 0.12 +0.8 4,177,971 - Pf6N2E2_3586 0.12 -0.7 4,178,106 + Pf6N2E2_3586 0.29 -1.6 4,178,106 + Pf6N2E2_3586 0.29 +0.1 4,178,297 + Pf6N2E2_3586 0.52 -0.4 4,178,297 + Pf6N2E2_3586 0.52 -1.2 4,178,315 + Pf6N2E2_3586 0.54 +0.9 4,178,315 + Pf6N2E2_3586 0.54 -2.3 4,178,315 + Pf6N2E2_3586 0.54 -0.6 4,178,316 - Pf6N2E2_3586 0.54 -3.0 4,178,328 + Pf6N2E2_3586 0.55 -0.4 4,178,328 + Pf6N2E2_3586 0.55 -2.0 4,178,328 + Pf6N2E2_3586 0.55 -3.2 4,178,328 + Pf6N2E2_3586 0.55 -1.3 4,178,328 + Pf6N2E2_3586 0.55 -1.6 4,178,328 + Pf6N2E2_3586 0.55 -2.0 4,178,329 - Pf6N2E2_3586 0.56 -0.6 4,178,329 - Pf6N2E2_3586 0.56 -0.9 4,178,329 - Pf6N2E2_3586 0.56 -1.2 4,178,580 + Pf6N2E2_3586 0.86 -1.5 4,178,580 + Pf6N2E2_3586 0.86 +0.5 4,178,580 + Pf6N2E2_3586 0.86 -0.4 4,178,581 - Pf6N2E2_3586 0.86 -1.6 4,178,652 + +0.5 4,178,652 + +0.3 4,178,699 - -0.3 4,178,799 + -0.3 4,178,981 - -0.2 4,179,080 + Pf6N2E2_3587 0.14 -1.0 4,179,081 - Pf6N2E2_3587 0.15 -0.5 4,179,188 + Pf6N2E2_3587 0.38 +0.5 4,179,188 + Pf6N2E2_3587 0.38 -1.0 4,179,188 + Pf6N2E2_3587 0.38 +1.3 4,179,188 + Pf6N2E2_3587 0.38 -0.1 4,179,189 - Pf6N2E2_3587 0.39 -2.1 4,179,189 - Pf6N2E2_3587 0.39 +0.4 4,179,440 - +3.3 4,179,534 + -0.3 4,179,564 + +0.1 4,179,564 + +0.8 4,179,571 - -0.2 4,179,571 - -1.4 4,179,618 + +0.4 4,179,619 - -1.0 4,179,619 - +0.2 4,179,619 - -2.4 4,179,619 - +0.8 4,179,673 + Pf6N2E2_3588 0.49 -0.6 4,179,673 + Pf6N2E2_3588 0.49 +0.9 4,179,674 - Pf6N2E2_3588 0.49 -0.7
Or see this region's nucleotide sequence