Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_337 and Pf6N2E2_338 are separated by 147 nucleotides Pf6N2E2_338 and Pf6N2E2_339 are separated by 6 nucleotides Pf6N2E2_339 and Pf6N2E2_340 are separated by 1 nucleotides Pf6N2E2_340 and Pf6N2E2_341 are separated by 177 nucleotides
Pf6N2E2_337 - hypothetical protein, at 404,587 to 404,859
_337
Pf6N2E2_338 - Transcriptional regulator, AraC family, at 405,007 to 405,510
_338
Pf6N2E2_339 - Glutathione-regulated potassium-efflux system protein KefC, at 405,517 to 407,343
_339
Pf6N2E2_340 - Glutathione-regulated potassium-efflux system ancillary protein KefF, at 407,345 to 407,866
_340
Pf6N2E2_341 - sensor histidine kinase, at 408,044 to 409,390
_341
Position (kb) 405
406
407
408 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 404.536 kb on + strand at 404.536 kb on + strand at 404.536 kb on + strand at 404.537 kb on - strand at 404.669 kb on - strand, within Pf6N2E2_337 at 404.875 kb on + strand at 404.875 kb on + strand at 404.876 kb on - strand at 405.098 kb on + strand, within Pf6N2E2_338 at 405.098 kb on + strand, within Pf6N2E2_338 at 405.732 kb on - strand, within Pf6N2E2_339 at 405.732 kb on - strand, within Pf6N2E2_339 at 406.027 kb on - strand, within Pf6N2E2_339 at 406.064 kb on - strand, within Pf6N2E2_339 at 407.188 kb on + strand at 407.189 kb on - strand at 407.189 kb on - strand at 407.239 kb on + strand at 407.240 kb on - strand at 407.240 kb on - strand at 407.240 kb on - strand at 407.240 kb on - strand at 407.240 kb on - strand at 407.243 kb on + strand at 407.244 kb on - strand at 407.309 kb on + strand at 407.309 kb on + strand at 407.309 kb on + strand at 407.310 kb on - strand at 407.310 kb on - strand at 407.310 kb on - strand at 407.379 kb on + strand at 407.379 kb on + strand at 407.380 kb on - strand at 407.380 kb on - strand at 407.380 kb on - strand at 407.380 kb on - strand at 407.380 kb on - strand at 407.421 kb on + strand, within Pf6N2E2_340 at 407.421 kb on + strand, within Pf6N2E2_340 at 407.466 kb on + strand, within Pf6N2E2_340 at 407.466 kb on + strand, within Pf6N2E2_340 at 407.466 kb on + strand, within Pf6N2E2_340 at 407.664 kb on + strand, within Pf6N2E2_340 at 407.670 kb on + strand, within Pf6N2E2_340 at 407.670 kb on + strand, within Pf6N2E2_340 at 407.670 kb on + strand, within Pf6N2E2_340 at 407.752 kb on - strand, within Pf6N2E2_340 at 407.760 kb on + strand, within Pf6N2E2_340 at 407.771 kb on + strand, within Pf6N2E2_340 at 407.771 kb on + strand, within Pf6N2E2_340 at 407.771 kb on + strand, within Pf6N2E2_340 at 407.772 kb on - strand, within Pf6N2E2_340 at 407.772 kb on - strand, within Pf6N2E2_340 at 407.772 kb on - strand, within Pf6N2E2_340 at 407.838 kb on + strand at 407.838 kb on + strand at 407.850 kb on + strand at 407.851 kb on - strand at 407.851 kb on - strand at 407.940 kb on + strand at 408.018 kb on + strand at 408.316 kb on - strand, within Pf6N2E2_341 at 408.316 kb on - strand, within Pf6N2E2_341 at 408.316 kb on - strand, within Pf6N2E2_341
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 404,536 + +1.5 404,536 + -0.3 404,536 + +1.6 404,537 - -0.5 404,669 - Pf6N2E2_337 0.30 +1.6 404,875 + +1.0 404,875 + -0.8 404,876 - +0.2 405,098 + Pf6N2E2_338 0.18 +1.4 405,098 + Pf6N2E2_338 0.18 -0.7 405,732 - Pf6N2E2_339 0.12 +2.8 405,732 - Pf6N2E2_339 0.12 +1.2 406,027 - Pf6N2E2_339 0.28 +0.6 406,064 - Pf6N2E2_339 0.30 +0.3 407,188 + +0.3 407,189 - +1.6 407,189 - +0.8 407,239 + -0.5 407,240 - +0.3 407,240 - -2.5 407,240 - -1.0 407,240 - +0.8 407,240 - -1.1 407,243 + -0.1 407,244 - -2.2 407,309 + +0.3 407,309 + -0.2 407,309 + +1.4 407,310 - -2.5 407,310 - +1.3 407,310 - -0.1 407,379 + +1.0 407,379 + -0.5 407,380 - +0.5 407,380 - +0.2 407,380 - -1.1 407,380 - +0.1 407,380 - +0.4 407,421 + Pf6N2E2_340 0.15 -0.6 407,421 + Pf6N2E2_340 0.15 -1.0 407,466 + Pf6N2E2_340 0.23 -1.5 407,466 + Pf6N2E2_340 0.23 +0.7 407,466 + Pf6N2E2_340 0.23 +0.8 407,664 + Pf6N2E2_340 0.61 -1.0 407,670 + Pf6N2E2_340 0.62 +0.5 407,670 + Pf6N2E2_340 0.62 -0.8 407,670 + Pf6N2E2_340 0.62 -0.9 407,752 - Pf6N2E2_340 0.78 -0.6 407,760 + Pf6N2E2_340 0.80 -1.0 407,771 + Pf6N2E2_340 0.82 -0.1 407,771 + Pf6N2E2_340 0.82 -0.2 407,771 + Pf6N2E2_340 0.82 +0.0 407,772 - Pf6N2E2_340 0.82 +0.4 407,772 - Pf6N2E2_340 0.82 -0.5 407,772 - Pf6N2E2_340 0.82 +0.9 407,838 + -0.9 407,838 + -0.3 407,850 + +0.7 407,851 - -0.5 407,851 - +1.0 407,940 + -0.8 408,018 + +1.4 408,316 - Pf6N2E2_341 0.20 -1.2 408,316 - Pf6N2E2_341 0.20 -1.3 408,316 - Pf6N2E2_341 0.20 -0.3
Or see this region's nucleotide sequence