Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3141 and Pf6N2E2_3142 are separated by 121 nucleotides Pf6N2E2_3142 and Pf6N2E2_3143 are separated by 104 nucleotides
Pf6N2E2_3141 - virulence sensor protein, at 3,633,161 to 3,634,531
_3141
Pf6N2E2_3142 - Threonine synthase (EC 4.2.3.1), at 3,634,653 to 3,636,062
_3142
Pf6N2E2_3143 - Homoserine dehydrogenase (EC 1.1.1.3), at 3,636,167 to 3,637,471
_3143
Position (kb)
3634
3635
3636
3637 Strain fitness (log2 ratio)
-1
0
1
2 at 3633.816 kb on + strand, within Pf6N2E2_3141 at 3633.817 kb on - strand, within Pf6N2E2_3141 at 3633.817 kb on - strand, within Pf6N2E2_3141 at 3633.817 kb on - strand, within Pf6N2E2_3141 at 3633.851 kb on + strand, within Pf6N2E2_3141 at 3633.851 kb on + strand, within Pf6N2E2_3141 at 3633.851 kb on + strand, within Pf6N2E2_3141 at 3633.852 kb on - strand, within Pf6N2E2_3141 at 3633.852 kb on - strand, within Pf6N2E2_3141 at 3633.874 kb on - strand, within Pf6N2E2_3141 at 3633.909 kb on + strand, within Pf6N2E2_3141 at 3633.909 kb on + strand, within Pf6N2E2_3141 at 3633.909 kb on + strand, within Pf6N2E2_3141 at 3633.910 kb on - strand, within Pf6N2E2_3141 at 3633.910 kb on - strand, within Pf6N2E2_3141 at 3633.948 kb on + strand, within Pf6N2E2_3141 at 3633.948 kb on + strand, within Pf6N2E2_3141 at 3633.949 kb on - strand, within Pf6N2E2_3141 at 3633.949 kb on - strand, within Pf6N2E2_3141 at 3633.949 kb on - strand, within Pf6N2E2_3141 at 3633.949 kb on - strand, within Pf6N2E2_3141 at 3633.949 kb on - strand, within Pf6N2E2_3141 at 3633.981 kb on + strand, within Pf6N2E2_3141 at 3634.091 kb on + strand, within Pf6N2E2_3141 at 3634.091 kb on + strand, within Pf6N2E2_3141 at 3634.092 kb on - strand, within Pf6N2E2_3141 at 3634.320 kb on + strand, within Pf6N2E2_3141 at 3634.321 kb on - strand, within Pf6N2E2_3141 at 3634.395 kb on - strand at 3636.169 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 3,633,816 + Pf6N2E2_3141 0.48 +1.9 3,633,817 - Pf6N2E2_3141 0.48 +0.2 3,633,817 - Pf6N2E2_3141 0.48 +0.6 3,633,817 - Pf6N2E2_3141 0.48 -0.5 3,633,851 + Pf6N2E2_3141 0.50 -1.7 3,633,851 + Pf6N2E2_3141 0.50 -0.7 3,633,851 + Pf6N2E2_3141 0.50 +0.2 3,633,852 - Pf6N2E2_3141 0.50 +0.4 3,633,852 - Pf6N2E2_3141 0.50 +1.8 3,633,874 - Pf6N2E2_3141 0.52 -0.7 3,633,909 + Pf6N2E2_3141 0.55 +1.7 3,633,909 + Pf6N2E2_3141 0.55 +0.2 3,633,909 + Pf6N2E2_3141 0.55 -1.0 3,633,910 - Pf6N2E2_3141 0.55 +0.3 3,633,910 - Pf6N2E2_3141 0.55 +1.9 3,633,948 + Pf6N2E2_3141 0.57 -1.7 3,633,948 + Pf6N2E2_3141 0.57 -1.0 3,633,949 - Pf6N2E2_3141 0.57 -1.7 3,633,949 - Pf6N2E2_3141 0.57 +1.1 3,633,949 - Pf6N2E2_3141 0.57 +0.9 3,633,949 - Pf6N2E2_3141 0.57 -1.3 3,633,949 - Pf6N2E2_3141 0.57 +0.4 3,633,981 + Pf6N2E2_3141 0.60 +0.1 3,634,091 + Pf6N2E2_3141 0.68 +0.8 3,634,091 + Pf6N2E2_3141 0.68 -0.4 3,634,092 - Pf6N2E2_3141 0.68 +0.5 3,634,320 + Pf6N2E2_3141 0.85 -0.4 3,634,321 - Pf6N2E2_3141 0.85 -0.0 3,634,395 - +0.2 3,636,169 - -0.9
Or see this region's nucleotide sequence