Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_23 and Pf6N2E2_24 are separated by 127 nucleotides Pf6N2E2_24 and Pf6N2E2_25 are separated by 75 nucleotides Pf6N2E2_25 and Pf6N2E2_26 are separated by 153 nucleotides
Pf6N2E2_23 - Hybrid sensory histidine kinase in two-component regulatory system with EvgA, at 58,372 to 61,572
_23
Pf6N2E2_24 - hypothetical protein, at 61,700 to 62,134
_24
Pf6N2E2_25 - DNA-binding response regulator, LuxR family, at 62,210 to 62,863
_25
Pf6N2E2_26 - Pirin, at 63,017 to 63,883
_26
Position (kb) 61
62
63 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7 at 60.783 kb on - strand, within Pf6N2E2_23 at 60.864 kb on + strand, within Pf6N2E2_23 at 61.498 kb on - strand at 61.498 kb on - strand at 61.570 kb on + strand at 61.570 kb on + strand at 61.571 kb on - strand at 61.571 kb on - strand at 61.571 kb on - strand at 61.571 kb on - strand at 61.603 kb on + strand at 61.609 kb on + strand at 61.609 kb on + strand at 61.610 kb on - strand at 61.610 kb on - strand at 61.649 kb on + strand at 61.650 kb on - strand at 61.650 kb on - strand at 61.684 kb on + strand at 61.685 kb on - strand at 61.787 kb on + strand, within Pf6N2E2_24 at 61.787 kb on + strand, within Pf6N2E2_24 at 61.788 kb on - strand, within Pf6N2E2_24 at 61.788 kb on - strand, within Pf6N2E2_24 at 61.842 kb on - strand, within Pf6N2E2_24 at 61.842 kb on - strand, within Pf6N2E2_24 at 61.918 kb on + strand, within Pf6N2E2_24 at 61.922 kb on + strand, within Pf6N2E2_24 at 61.922 kb on + strand, within Pf6N2E2_24 at 61.922 kb on + strand, within Pf6N2E2_24 at 61.922 kb on + strand, within Pf6N2E2_24 at 61.923 kb on - strand, within Pf6N2E2_24 at 61.923 kb on - strand, within Pf6N2E2_24 at 61.923 kb on - strand, within Pf6N2E2_24 at 61.923 kb on - strand, within Pf6N2E2_24 at 61.923 kb on - strand, within Pf6N2E2_24 at 61.923 kb on - strand, within Pf6N2E2_24 at 61.923 kb on - strand, within Pf6N2E2_24 at 61.952 kb on + strand, within Pf6N2E2_24 at 61.952 kb on + strand, within Pf6N2E2_24 at 61.953 kb on - strand, within Pf6N2E2_24 at 62.229 kb on + strand at 62.229 kb on + strand at 62.229 kb on + strand at 62.230 kb on - strand at 62.230 kb on - strand at 62.287 kb on - strand, within Pf6N2E2_25 at 62.287 kb on - strand, within Pf6N2E2_25 at 62.305 kb on + strand, within Pf6N2E2_25 at 62.305 kb on + strand, within Pf6N2E2_25 at 62.305 kb on + strand, within Pf6N2E2_25 at 62.306 kb on - strand, within Pf6N2E2_25 at 62.306 kb on - strand, within Pf6N2E2_25 at 62.616 kb on - strand, within Pf6N2E2_25 at 62.783 kb on + strand, within Pf6N2E2_25 at 62.783 kb on + strand, within Pf6N2E2_25 at 62.783 kb on + strand, within Pf6N2E2_25 at 62.783 kb on + strand, within Pf6N2E2_25 at 62.784 kb on - strand, within Pf6N2E2_25 at 62.784 kb on - strand, within Pf6N2E2_25 at 62.784 kb on - strand, within Pf6N2E2_25 at 62.784 kb on - strand, within Pf6N2E2_25 at 62.784 kb on - strand, within Pf6N2E2_25 at 62.880 kb on + strand at 62.880 kb on + strand at 62.881 kb on - strand at 62.895 kb on - strand at 62.921 kb on - strand at 62.929 kb on - strand at 62.948 kb on + strand at 62.949 kb on - strand at 62.971 kb on + strand at 62.972 kb on - strand at 62.972 kb on - strand at 62.972 kb on - strand at 62.996 kb on - strand at 62.996 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 60,783 - Pf6N2E2_23 0.75 -0.5 60,864 + Pf6N2E2_23 0.78 +0.1 61,498 - +0.8 61,498 - +0.2 61,570 + +2.9 61,570 + +2.0 61,571 - -2.1 61,571 - -0.7 61,571 - +0.9 61,571 - +0.1 61,603 + +1.0 61,609 + +1.6 61,609 + -0.9 61,610 - +1.4 61,610 - +0.2 61,649 + +7.0 61,650 - -1.4 61,650 - -0.2 61,684 + +5.9 61,685 - +2.0 61,787 + Pf6N2E2_24 0.20 -1.8 61,787 + Pf6N2E2_24 0.20 +1.1 61,788 - Pf6N2E2_24 0.20 -0.4 61,788 - Pf6N2E2_24 0.20 -0.9 61,842 - Pf6N2E2_24 0.33 +1.3 61,842 - Pf6N2E2_24 0.33 -2.3 61,918 + Pf6N2E2_24 0.50 -0.8 61,922 + Pf6N2E2_24 0.51 -1.8 61,922 + Pf6N2E2_24 0.51 -1.2 61,922 + Pf6N2E2_24 0.51 +1.5 61,922 + Pf6N2E2_24 0.51 -0.8 61,923 - Pf6N2E2_24 0.51 +0.1 61,923 - Pf6N2E2_24 0.51 -0.9 61,923 - Pf6N2E2_24 0.51 -0.0 61,923 - Pf6N2E2_24 0.51 -0.8 61,923 - Pf6N2E2_24 0.51 -0.2 61,923 - Pf6N2E2_24 0.51 +1.0 61,923 - Pf6N2E2_24 0.51 -1.7 61,952 + Pf6N2E2_24 0.58 -0.4 61,952 + Pf6N2E2_24 0.58 -1.2 61,953 - Pf6N2E2_24 0.58 -1.0 62,229 + -1.1 62,229 + -2.4 62,229 + +1.8 62,230 - +1.0 62,230 - +1.8 62,287 - Pf6N2E2_25 0.12 -0.4 62,287 - Pf6N2E2_25 0.12 +3.1 62,305 + Pf6N2E2_25 0.15 -2.1 62,305 + Pf6N2E2_25 0.15 -1.0 62,305 + Pf6N2E2_25 0.15 -1.9 62,306 - Pf6N2E2_25 0.15 -2.7 62,306 - Pf6N2E2_25 0.15 +0.8 62,616 - Pf6N2E2_25 0.62 +0.7 62,783 + Pf6N2E2_25 0.88 -0.4 62,783 + Pf6N2E2_25 0.88 +0.7 62,783 + Pf6N2E2_25 0.88 -0.1 62,783 + Pf6N2E2_25 0.88 -0.1 62,784 - Pf6N2E2_25 0.88 -1.2 62,784 - Pf6N2E2_25 0.88 +1.6 62,784 - Pf6N2E2_25 0.88 +1.5 62,784 - Pf6N2E2_25 0.88 +1.3 62,784 - Pf6N2E2_25 0.88 -0.0 62,880 + +1.6 62,880 + -1.0 62,881 - -3.7 62,895 - -0.9 62,921 - -0.2 62,929 - -0.8 62,948 + +1.8 62,949 - -0.9 62,971 + -0.8 62,972 - +0.0 62,972 - -0.9 62,972 - -2.2 62,996 - -0.2 62,996 - -2.7
Or see this region's nucleotide sequence