Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2187 and Pf6N2E2_2188 are separated by 197 nucleotides Pf6N2E2_2188 and Pf6N2E2_2189 are separated by 92 nucleotides
Pf6N2E2_2187 - Transcriptional regulator, LysR family, at 2,591,642 to 2,592,571
_2187
Pf6N2E2_2188 - Predicted transcriptional regulator LiuR of leucine degradation pathway, MerR family, at 2,592,769 to 2,593,170
_2188
Pf6N2E2_2189 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 2,593,263 to 2,594,162
_2189
Position (kb)
2592
2593
2594 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2592.013 kb on + strand, within Pf6N2E2_2187 at 2592.013 kb on + strand, within Pf6N2E2_2187 at 2592.014 kb on - strand, within Pf6N2E2_2187 at 2592.014 kb on - strand, within Pf6N2E2_2187 at 2592.014 kb on - strand, within Pf6N2E2_2187 at 2592.123 kb on + strand, within Pf6N2E2_2187 at 2592.152 kb on + strand, within Pf6N2E2_2187 at 2592.152 kb on + strand, within Pf6N2E2_2187 at 2592.153 kb on - strand, within Pf6N2E2_2187 at 2592.153 kb on - strand, within Pf6N2E2_2187 at 2592.215 kb on + strand, within Pf6N2E2_2187 at 2592.238 kb on + strand, within Pf6N2E2_2187 at 2592.238 kb on + strand, within Pf6N2E2_2187 at 2592.239 kb on - strand, within Pf6N2E2_2187 at 2592.239 kb on - strand, within Pf6N2E2_2187 at 2592.266 kb on + strand, within Pf6N2E2_2187 at 2592.267 kb on - strand, within Pf6N2E2_2187 at 2592.267 kb on - strand, within Pf6N2E2_2187 at 2592.267 kb on - strand, within Pf6N2E2_2187 at 2592.329 kb on + strand, within Pf6N2E2_2187 at 2592.329 kb on + strand, within Pf6N2E2_2187 at 2592.329 kb on + strand, within Pf6N2E2_2187 at 2592.329 kb on + strand, within Pf6N2E2_2187 at 2592.329 kb on + strand, within Pf6N2E2_2187 at 2592.330 kb on - strand, within Pf6N2E2_2187 at 2592.330 kb on - strand, within Pf6N2E2_2187 at 2592.570 kb on - strand at 2592.631 kb on - strand at 2592.631 kb on - strand at 2592.643 kb on - strand at 2592.721 kb on - strand at 2592.732 kb on + strand at 2592.737 kb on + strand at 2592.784 kb on + strand at 2592.784 kb on + strand at 2592.785 kb on - strand at 2592.785 kb on - strand at 2592.785 kb on - strand at 2592.785 kb on - strand at 2592.841 kb on + strand, within Pf6N2E2_2188 at 2592.842 kb on - strand, within Pf6N2E2_2188 at 2592.842 kb on - strand, within Pf6N2E2_2188 at 2592.892 kb on + strand, within Pf6N2E2_2188 at 2592.986 kb on - strand, within Pf6N2E2_2188 at 2592.986 kb on - strand, within Pf6N2E2_2188 at 2592.986 kb on - strand, within Pf6N2E2_2188 at 2593.169 kb on - strand at 2593.234 kb on + strand at 2593.234 kb on + strand at 2593.234 kb on + strand at 2593.235 kb on - strand at 2593.235 kb on - strand at 2593.283 kb on - strand at 2593.283 kb on - strand at 2593.392 kb on + strand, within Pf6N2E2_2189 at 2593.486 kb on + strand, within Pf6N2E2_2189 at 2593.486 kb on + strand, within Pf6N2E2_2189 at 2593.491 kb on + strand, within Pf6N2E2_2189 at 2593.491 kb on + strand, within Pf6N2E2_2189 at 2593.492 kb on - strand, within Pf6N2E2_2189 at 2593.762 kb on - strand, within Pf6N2E2_2189 at 2593.762 kb on - strand, within Pf6N2E2_2189 at 2593.776 kb on + strand, within Pf6N2E2_2189 at 2593.896 kb on + strand, within Pf6N2E2_2189 at 2593.896 kb on + strand, within Pf6N2E2_2189 at 2593.896 kb on + strand, within Pf6N2E2_2189 at 2593.897 kb on - strand, within Pf6N2E2_2189 at 2593.986 kb on + strand, within Pf6N2E2_2189 at 2594.031 kb on + strand, within Pf6N2E2_2189 at 2594.031 kb on + strand, within Pf6N2E2_2189 at 2594.032 kb on - strand, within Pf6N2E2_2189 at 2594.032 kb on - strand, within Pf6N2E2_2189 at 2594.032 kb on - strand, within Pf6N2E2_2189 at 2594.032 kb on - strand, within Pf6N2E2_2189 at 2594.032 kb on - strand, within Pf6N2E2_2189
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 2,592,013 + Pf6N2E2_2187 0.40 -1.1 2,592,013 + Pf6N2E2_2187 0.40 -0.2 2,592,014 - Pf6N2E2_2187 0.40 +0.2 2,592,014 - Pf6N2E2_2187 0.40 +0.2 2,592,014 - Pf6N2E2_2187 0.40 +3.0 2,592,123 + Pf6N2E2_2187 0.52 +0.4 2,592,152 + Pf6N2E2_2187 0.55 -0.6 2,592,152 + Pf6N2E2_2187 0.55 -0.2 2,592,153 - Pf6N2E2_2187 0.55 +1.0 2,592,153 - Pf6N2E2_2187 0.55 -0.4 2,592,215 + Pf6N2E2_2187 0.62 -0.8 2,592,238 + Pf6N2E2_2187 0.64 -0.2 2,592,238 + Pf6N2E2_2187 0.64 +0.1 2,592,239 - Pf6N2E2_2187 0.64 -1.0 2,592,239 - Pf6N2E2_2187 0.64 -0.2 2,592,266 + Pf6N2E2_2187 0.67 +1.0 2,592,267 - Pf6N2E2_2187 0.67 -0.3 2,592,267 - Pf6N2E2_2187 0.67 +0.9 2,592,267 - Pf6N2E2_2187 0.67 +1.0 2,592,329 + Pf6N2E2_2187 0.74 -2.6 2,592,329 + Pf6N2E2_2187 0.74 -1.7 2,592,329 + Pf6N2E2_2187 0.74 +0.5 2,592,329 + Pf6N2E2_2187 0.74 -0.3 2,592,329 + Pf6N2E2_2187 0.74 -0.1 2,592,330 - Pf6N2E2_2187 0.74 +0.1 2,592,330 - Pf6N2E2_2187 0.74 +0.5 2,592,570 - -0.1 2,592,631 - -1.5 2,592,631 - +0.6 2,592,643 - +0.7 2,592,721 - +0.1 2,592,732 + +1.0 2,592,737 + +1.8 2,592,784 + +0.1 2,592,784 + -1.1 2,592,785 - -3.0 2,592,785 - -2.3 2,592,785 - -1.1 2,592,785 - -0.6 2,592,841 + Pf6N2E2_2188 0.18 -3.1 2,592,842 - Pf6N2E2_2188 0.18 +0.7 2,592,842 - Pf6N2E2_2188 0.18 -1.3 2,592,892 + Pf6N2E2_2188 0.31 -0.5 2,592,986 - Pf6N2E2_2188 0.54 -2.7 2,592,986 - Pf6N2E2_2188 0.54 -2.2 2,592,986 - Pf6N2E2_2188 0.54 -0.4 2,593,169 - +0.1 2,593,234 + -1.6 2,593,234 + +0.2 2,593,234 + +1.0 2,593,235 - +1.1 2,593,235 - -0.3 2,593,283 - -0.3 2,593,283 - -0.3 2,593,392 + Pf6N2E2_2189 0.14 -1.6 2,593,486 + Pf6N2E2_2189 0.25 -0.1 2,593,486 + Pf6N2E2_2189 0.25 +0.7 2,593,491 + Pf6N2E2_2189 0.25 +0.1 2,593,491 + Pf6N2E2_2189 0.25 -0.6 2,593,492 - Pf6N2E2_2189 0.25 +0.6 2,593,762 - Pf6N2E2_2189 0.55 -0.6 2,593,762 - Pf6N2E2_2189 0.55 -0.3 2,593,776 + Pf6N2E2_2189 0.57 +1.7 2,593,896 + Pf6N2E2_2189 0.70 +0.5 2,593,896 + Pf6N2E2_2189 0.70 -0.3 2,593,896 + Pf6N2E2_2189 0.70 -1.5 2,593,897 - Pf6N2E2_2189 0.70 +0.1 2,593,986 + Pf6N2E2_2189 0.80 -0.3 2,594,031 + Pf6N2E2_2189 0.85 +1.5 2,594,031 + Pf6N2E2_2189 0.85 +1.5 2,594,032 - Pf6N2E2_2189 0.85 +0.5 2,594,032 - Pf6N2E2_2189 0.85 -0.3 2,594,032 - Pf6N2E2_2189 0.85 +0.1 2,594,032 - Pf6N2E2_2189 0.85 +2.1 2,594,032 - Pf6N2E2_2189 0.85 +2.0
Or see this region's nucleotide sequence