Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1798 and Pf6N2E2_1799 are separated by 140 nucleotides Pf6N2E2_1799 and Pf6N2E2_1800 are separated by 10 nucleotides Pf6N2E2_1800 and Pf6N2E2_1801 are separated by 39 nucleotides
Pf6N2E2_1798 - Argininosuccinate lyase (EC 4.3.2.1), at 2,127,479 to 2,128,906
_1798
Pf6N2E2_1799 - ABC transporter permease protein, at 2,129,047 to 2,129,925
_1799
Pf6N2E2_1800 - putative ABC transporter ATP-binding protein, at 2,129,936 to 2,130,700
_1800
Pf6N2E2_1801 - ABC-type polar amino acid transport system protein, substrate-binding protein, at 2,130,740 to 2,131,564
_1801
Position (kb)
2129
2130 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2128.054 kb on - strand, within Pf6N2E2_1798 at 2128.054 kb on - strand, within Pf6N2E2_1798 at 2128.057 kb on + strand, within Pf6N2E2_1798 at 2128.057 kb on + strand, within Pf6N2E2_1798 at 2128.057 kb on + strand, within Pf6N2E2_1798 at 2128.057 kb on + strand, within Pf6N2E2_1798 at 2128.057 kb on + strand, within Pf6N2E2_1798 at 2128.058 kb on - strand, within Pf6N2E2_1798 at 2128.058 kb on - strand, within Pf6N2E2_1798 at 2128.058 kb on - strand, within Pf6N2E2_1798 at 2128.058 kb on - strand, within Pf6N2E2_1798 at 2128.235 kb on - strand, within Pf6N2E2_1798 at 2128.439 kb on + strand, within Pf6N2E2_1798 at 2128.886 kb on + strand at 2128.887 kb on - strand at 2129.144 kb on - strand, within Pf6N2E2_1799 at 2129.230 kb on + strand, within Pf6N2E2_1799 at 2129.390 kb on - strand, within Pf6N2E2_1799 at 2129.390 kb on - strand, within Pf6N2E2_1799 at 2129.390 kb on - strand, within Pf6N2E2_1799 at 2129.560 kb on + strand, within Pf6N2E2_1799 at 2129.561 kb on - strand, within Pf6N2E2_1799 at 2129.589 kb on - strand, within Pf6N2E2_1799 at 2129.774 kb on - strand, within Pf6N2E2_1799 at 2129.774 kb on - strand, within Pf6N2E2_1799 at 2129.791 kb on + strand, within Pf6N2E2_1799 at 2129.792 kb on - strand, within Pf6N2E2_1799 at 2129.972 kb on + strand at 2129.972 kb on + strand at 2129.973 kb on - strand at 2129.973 kb on - strand at 2130.648 kb on + strand at 2130.648 kb on + strand at 2130.648 kb on + strand at 2130.648 kb on + strand at 2130.648 kb on + strand at 2130.698 kb on + strand at 2130.699 kb on - strand at 2130.917 kb on + strand, within Pf6N2E2_1801 at 2130.918 kb on - strand, within Pf6N2E2_1801 at 2130.918 kb on - strand, within Pf6N2E2_1801 at 2130.918 kb on - strand, within Pf6N2E2_1801
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 2,128,054 - Pf6N2E2_1798 0.40 +1.5 2,128,054 - Pf6N2E2_1798 0.40 -0.5 2,128,057 + Pf6N2E2_1798 0.40 -1.5 2,128,057 + Pf6N2E2_1798 0.40 +0.5 2,128,057 + Pf6N2E2_1798 0.40 +1.7 2,128,057 + Pf6N2E2_1798 0.40 +0.4 2,128,057 + Pf6N2E2_1798 0.40 +1.5 2,128,058 - Pf6N2E2_1798 0.41 -0.7 2,128,058 - Pf6N2E2_1798 0.41 +0.5 2,128,058 - Pf6N2E2_1798 0.41 +0.6 2,128,058 - Pf6N2E2_1798 0.41 -1.1 2,128,235 - Pf6N2E2_1798 0.53 +1.3 2,128,439 + Pf6N2E2_1798 0.67 -1.7 2,128,886 + -0.1 2,128,887 - -0.1 2,129,144 - Pf6N2E2_1799 0.11 -2.3 2,129,230 + Pf6N2E2_1799 0.21 -0.9 2,129,390 - Pf6N2E2_1799 0.39 -1.3 2,129,390 - Pf6N2E2_1799 0.39 -1.9 2,129,390 - Pf6N2E2_1799 0.39 -1.2 2,129,560 + Pf6N2E2_1799 0.58 +0.9 2,129,561 - Pf6N2E2_1799 0.58 -1.0 2,129,589 - Pf6N2E2_1799 0.62 -0.1 2,129,774 - Pf6N2E2_1799 0.83 -0.7 2,129,774 - Pf6N2E2_1799 0.83 -0.4 2,129,791 + Pf6N2E2_1799 0.85 +1.7 2,129,792 - Pf6N2E2_1799 0.85 -2.2 2,129,972 + +2.2 2,129,972 + -2.3 2,129,973 - +0.0 2,129,973 - +1.5 2,130,648 + +0.8 2,130,648 + -0.2 2,130,648 + +1.3 2,130,648 + +0.1 2,130,648 + +0.5 2,130,698 + -0.2 2,130,699 - +1.2 2,130,917 + Pf6N2E2_1801 0.21 +0.7 2,130,918 - Pf6N2E2_1801 0.22 -1.2 2,130,918 - Pf6N2E2_1801 0.22 -0.9 2,130,918 - Pf6N2E2_1801 0.22 +0.6
Or see this region's nucleotide sequence