Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1467 and Pf6N2E2_1468 overlap by 4 nucleotides Pf6N2E2_1468 and Pf6N2E2_1469 overlap by 7 nucleotides
Pf6N2E2_1467 - NAD-dependent formate dehydrogenase alpha subunit, at 1,714,851 to 1,717,727
_1467
Pf6N2E2_1468 - NAD-dependent formate dehydrogenase beta subunit, at 1,717,724 to 1,719,283
_1468
Pf6N2E2_1469 - NAD-dependent formate dehydrogenase gamma subunit, at 1,719,277 to 1,719,759
_1469
Position (kb)
1717
1718
1719
1720 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1716.725 kb on - strand, within Pf6N2E2_1467 at 1716.760 kb on + strand, within Pf6N2E2_1467 at 1716.979 kb on + strand, within Pf6N2E2_1467 at 1716.992 kb on + strand, within Pf6N2E2_1467 at 1717.393 kb on + strand, within Pf6N2E2_1467 at 1717.394 kb on - strand, within Pf6N2E2_1467 at 1717.394 kb on - strand, within Pf6N2E2_1467 at 1717.404 kb on + strand, within Pf6N2E2_1467 at 1717.405 kb on - strand, within Pf6N2E2_1467 at 1717.405 kb on - strand, within Pf6N2E2_1467 at 1717.405 kb on - strand, within Pf6N2E2_1467 at 1717.830 kb on + strand at 1717.831 kb on - strand at 1718.085 kb on + strand, within Pf6N2E2_1468 at 1718.311 kb on - strand, within Pf6N2E2_1468 at 1718.482 kb on + strand, within Pf6N2E2_1468 at 1718.482 kb on + strand, within Pf6N2E2_1468 at 1718.483 kb on - strand, within Pf6N2E2_1468 at 1718.485 kb on - strand, within Pf6N2E2_1468 at 1718.485 kb on - strand, within Pf6N2E2_1468 at 1718.485 kb on - strand, within Pf6N2E2_1468 at 1718.594 kb on - strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.626 kb on - strand, within Pf6N2E2_1468 at 1718.626 kb on - strand, within Pf6N2E2_1468 at 1718.626 kb on - strand, within Pf6N2E2_1468 at 1718.626 kb on - strand, within Pf6N2E2_1468 at 1718.631 kb on + strand, within Pf6N2E2_1468 at 1718.631 kb on + strand, within Pf6N2E2_1468 at 1718.631 kb on + strand, within Pf6N2E2_1468 at 1718.632 kb on - strand, within Pf6N2E2_1468 at 1718.760 kb on + strand, within Pf6N2E2_1468 at 1718.761 kb on - strand, within Pf6N2E2_1468 at 1718.761 kb on - strand, within Pf6N2E2_1468 at 1718.761 kb on - strand, within Pf6N2E2_1468 at 1718.761 kb on - strand, within Pf6N2E2_1468 at 1718.761 kb on - strand, within Pf6N2E2_1468 at 1718.925 kb on + strand, within Pf6N2E2_1468 at 1719.147 kb on + strand at 1719.147 kb on + strand at 1719.147 kb on + strand at 1719.185 kb on + strand at 1719.185 kb on + strand at 1719.185 kb on + strand at 1719.268 kb on + strand at 1719.268 kb on + strand at 1719.399 kb on - strand, within Pf6N2E2_1469 at 1719.531 kb on + strand, within Pf6N2E2_1469 at 1719.566 kb on + strand, within Pf6N2E2_1469 at 1719.567 kb on - strand, within Pf6N2E2_1469 at 1719.647 kb on + strand, within Pf6N2E2_1469 at 1719.648 kb on - strand, within Pf6N2E2_1469 at 1719.648 kb on - strand, within Pf6N2E2_1469 at 1719.678 kb on + strand, within Pf6N2E2_1469 at 1719.679 kb on - strand, within Pf6N2E2_1469 at 1719.759 kb on + strand at 1719.759 kb on + strand at 1719.760 kb on - strand at 1719.760 kb on - strand at 1719.765 kb on + strand at 1719.765 kb on + strand at 1719.766 kb on - strand at 1719.766 kb on - strand at 1720.133 kb on - strand at 1720.133 kb on - strand at 1720.134 kb on + strand at 1720.135 kb on - strand at 1720.135 kb on - strand at 1720.135 kb on - strand at 1720.135 kb on - strand at 1720.136 kb on + strand at 1720.136 kb on + strand at 1720.137 kb on - strand at 1720.137 kb on - strand at 1720.137 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 1,716,725 - Pf6N2E2_1467 0.65 -0.6 1,716,760 + Pf6N2E2_1467 0.66 +0.1 1,716,979 + Pf6N2E2_1467 0.74 +0.1 1,716,992 + Pf6N2E2_1467 0.74 +0.5 1,717,393 + Pf6N2E2_1467 0.88 +0.6 1,717,394 - Pf6N2E2_1467 0.88 +0.1 1,717,394 - Pf6N2E2_1467 0.88 +0.3 1,717,404 + Pf6N2E2_1467 0.89 -1.3 1,717,405 - Pf6N2E2_1467 0.89 -0.9 1,717,405 - Pf6N2E2_1467 0.89 -0.5 1,717,405 - Pf6N2E2_1467 0.89 +1.3 1,717,830 + -0.3 1,717,831 - +1.3 1,718,085 + Pf6N2E2_1468 0.23 -0.7 1,718,311 - Pf6N2E2_1468 0.38 -0.5 1,718,482 + Pf6N2E2_1468 0.49 +2.6 1,718,482 + Pf6N2E2_1468 0.49 -1.9 1,718,483 - Pf6N2E2_1468 0.49 -1.9 1,718,485 - Pf6N2E2_1468 0.49 -0.2 1,718,485 - Pf6N2E2_1468 0.49 +0.1 1,718,485 - Pf6N2E2_1468 0.49 -0.5 1,718,594 - Pf6N2E2_1468 0.56 +0.5 1,718,625 + Pf6N2E2_1468 0.58 +1.0 1,718,625 + Pf6N2E2_1468 0.58 +0.5 1,718,625 + Pf6N2E2_1468 0.58 +1.0 1,718,625 + Pf6N2E2_1468 0.58 +0.7 1,718,625 + Pf6N2E2_1468 0.58 -0.7 1,718,626 - Pf6N2E2_1468 0.58 -1.0 1,718,626 - Pf6N2E2_1468 0.58 -1.4 1,718,626 - Pf6N2E2_1468 0.58 -1.7 1,718,626 - Pf6N2E2_1468 0.58 -2.4 1,718,631 + Pf6N2E2_1468 0.58 +1.2 1,718,631 + Pf6N2E2_1468 0.58 +1.3 1,718,631 + Pf6N2E2_1468 0.58 -1.7 1,718,632 - Pf6N2E2_1468 0.58 +0.3 1,718,760 + Pf6N2E2_1468 0.66 +0.1 1,718,761 - Pf6N2E2_1468 0.66 -1.3 1,718,761 - Pf6N2E2_1468 0.66 -2.7 1,718,761 - Pf6N2E2_1468 0.66 -1.0 1,718,761 - Pf6N2E2_1468 0.66 -1.5 1,718,761 - Pf6N2E2_1468 0.66 -0.8 1,718,925 + Pf6N2E2_1468 0.77 +1.0 1,719,147 + -0.7 1,719,147 + -0.2 1,719,147 + +0.4 1,719,185 + +0.3 1,719,185 + -1.0 1,719,185 + -0.2 1,719,268 + -0.8 1,719,268 + -3.8 1,719,399 - Pf6N2E2_1469 0.25 +0.8 1,719,531 + Pf6N2E2_1469 0.53 -1.5 1,719,566 + Pf6N2E2_1469 0.60 -0.3 1,719,567 - Pf6N2E2_1469 0.60 +0.1 1,719,647 + Pf6N2E2_1469 0.77 -2.6 1,719,648 - Pf6N2E2_1469 0.77 -0.7 1,719,648 - Pf6N2E2_1469 0.77 -0.7 1,719,678 + Pf6N2E2_1469 0.83 +1.8 1,719,679 - Pf6N2E2_1469 0.83 -1.0 1,719,759 + -2.2 1,719,759 + -0.4 1,719,760 - +0.5 1,719,760 - -0.3 1,719,765 + +1.8 1,719,765 + -1.7 1,719,766 - +1.3 1,719,766 - +1.3 1,720,133 - -0.3 1,720,133 - -1.0 1,720,134 + +0.3 1,720,135 - -0.3 1,720,135 - -1.3 1,720,135 - +1.6 1,720,135 - +0.8 1,720,136 + +0.9 1,720,136 + -2.0 1,720,137 - -0.3 1,720,137 - -1.9 1,720,137 - -0.8
Or see this region's nucleotide sequence