Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1215

Experiment: L-Serine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1212 and Pf6N2E2_1213 are separated by 79 nucleotidesPf6N2E2_1213 and Pf6N2E2_1214 are separated by 9 nucleotidesPf6N2E2_1214 and Pf6N2E2_1215 are separated by 168 nucleotidesPf6N2E2_1215 and Pf6N2E2_1216 are separated by 51 nucleotides Pf6N2E2_1212 - Oxidoreductase, short chain dehydrogenase/reductase family, at 1,418,735 to 1,419,592 _1212 Pf6N2E2_1213 - hypothetical protein, at 1,419,672 to 1,419,794 _1213 Pf6N2E2_1214 - C-terminal domain of CinA type S; Protein Implicated in DNA repair function with RecA and MutS, at 1,419,804 to 1,420,304 _1214 Pf6N2E2_1215 - probable membrane protein YPO2362, at 1,420,473 to 1,421,084 _1215 Pf6N2E2_1216 - ATP-dependent DNA ligase (EC 6.5.1.1) clustered with Ku protein, LigD, at 1,421,136 to 1,423,772 _1216 Position (kb) 1420 1421 1422Strain fitness (log2 ratio) -2 -1 0 1 2at 1419.512 kb on + strandat 1419.512 kb on + strandat 1419.542 kb on + strandat 1419.542 kb on + strandat 1419.542 kb on + strandat 1419.542 kb on + strandat 1419.542 kb on + strandat 1419.543 kb on - strandat 1419.543 kb on - strandat 1419.543 kb on - strandat 1419.585 kb on + strandat 1419.586 kb on - strandat 1419.688 kb on + strand, within Pf6N2E2_1213at 1419.688 kb on + strand, within Pf6N2E2_1213at 1419.688 kb on + strand, within Pf6N2E2_1213at 1419.689 kb on - strand, within Pf6N2E2_1213at 1419.689 kb on - strand, within Pf6N2E2_1213at 1420.157 kb on - strand, within Pf6N2E2_1214at 1420.276 kb on + strandat 1420.277 kb on - strandat 1420.277 kb on - strandat 1420.277 kb on - strandat 1420.528 kb on + strandat 1420.528 kb on + strandat 1420.528 kb on + strandat 1420.529 kb on - strandat 1420.529 kb on - strandat 1420.529 kb on - strandat 1420.529 kb on - strandat 1420.560 kb on + strand, within Pf6N2E2_1215at 1420.601 kb on - strand, within Pf6N2E2_1215at 1420.702 kb on - strand, within Pf6N2E2_1215at 1420.702 kb on - strand, within Pf6N2E2_1215at 1420.810 kb on - strand, within Pf6N2E2_1215at 1421.046 kb on + strandat 1421.181 kb on - strandat 1421.383 kb on + strandat 1421.578 kb on - strand, within Pf6N2E2_1216at 1421.938 kb on + strand, within Pf6N2E2_1216at 1421.938 kb on + strand, within Pf6N2E2_1216at 1421.939 kb on - strand, within Pf6N2E2_1216

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Serine (N)
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1,419,512 + -1.6
1,419,512 + +0.5
1,419,542 + +0.5
1,419,542 + +1.8
1,419,542 + +0.1
1,419,542 + +1.9
1,419,542 + +0.1
1,419,543 - +1.3
1,419,543 - -0.1
1,419,543 - -1.2
1,419,585 + -1.1
1,419,586 - -0.3
1,419,688 + Pf6N2E2_1213 0.13 -2.1
1,419,688 + Pf6N2E2_1213 0.13 -1.0
1,419,688 + Pf6N2E2_1213 0.13 +0.4
1,419,689 - Pf6N2E2_1213 0.14 +0.3
1,419,689 - Pf6N2E2_1213 0.14 -1.2
1,420,157 - Pf6N2E2_1214 0.70 +1.6
1,420,276 + +1.3
1,420,277 - +0.8
1,420,277 - -0.8
1,420,277 - -0.6
1,420,528 + -0.9
1,420,528 + +0.0
1,420,528 + -1.6
1,420,529 - +1.7
1,420,529 - -0.5
1,420,529 - -0.0
1,420,529 - +0.6
1,420,560 + Pf6N2E2_1215 0.14 -0.8
1,420,601 - Pf6N2E2_1215 0.21 -1.7
1,420,702 - Pf6N2E2_1215 0.37 -0.3
1,420,702 - Pf6N2E2_1215 0.37 -0.7
1,420,810 - Pf6N2E2_1215 0.55 -1.9
1,421,046 + -0.2
1,421,181 - +0.0
1,421,383 + -0.1
1,421,578 - Pf6N2E2_1216 0.17 +1.3
1,421,938 + Pf6N2E2_1216 0.30 +0.7
1,421,938 + Pf6N2E2_1216 0.30 -0.5
1,421,939 - Pf6N2E2_1216 0.30 +0.8

Or see this region's nucleotide sequence