Experiment: L-Serine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1187 and Pf6N2E2_1188 are separated by 9 nucleotides Pf6N2E2_1188 and Pf6N2E2_1189 overlap by 10 nucleotides Pf6N2E2_1189 and Pf6N2E2_1190 are separated by 1 nucleotides
Pf6N2E2_1187 - Probable nikkomycin biosynthesis protein, carboxylase, at 1,389,990 to 1,391,273
_1187
Pf6N2E2_1188 - Alpha-ketoglutarate-dependent taurine dioxygenase (EC 1.14.11.17), at 1,391,283 to 1,392,140
_1188
Pf6N2E2_1189 - Thioredoxin reductase (EC 1.8.1.9), at 1,392,131 to 1,393,300
_1189
Pf6N2E2_1190 - Glutamine-dependent 2-keto-4-methylthiobutyrate transaminase, at 1,393,302 to 1,394,303
_1190
Position (kb)
1392
1393
1394 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1391.135 kb on + strand, within Pf6N2E2_1187 at 1391.135 kb on + strand, within Pf6N2E2_1187 at 1391.171 kb on - strand at 1391.234 kb on + strand at 1391.239 kb on + strand at 1391.239 kb on + strand at 1391.273 kb on + strand at 1391.273 kb on + strand at 1391.274 kb on - strand at 1391.276 kb on + strand at 1391.276 kb on + strand at 1391.276 kb on + strand at 1391.276 kb on + strand at 1391.277 kb on - strand at 1391.277 kb on - strand at 1391.360 kb on + strand at 1391.496 kb on + strand, within Pf6N2E2_1188 at 1391.524 kb on + strand, within Pf6N2E2_1188 at 1391.525 kb on - strand, within Pf6N2E2_1188 at 1391.525 kb on - strand, within Pf6N2E2_1188 at 1391.558 kb on - strand, within Pf6N2E2_1188 at 1391.803 kb on - strand, within Pf6N2E2_1188 at 1391.863 kb on + strand, within Pf6N2E2_1188 at 1392.004 kb on + strand, within Pf6N2E2_1188 at 1392.004 kb on + strand, within Pf6N2E2_1188 at 1392.005 kb on - strand, within Pf6N2E2_1188 at 1392.005 kb on - strand, within Pf6N2E2_1188 at 1392.043 kb on - strand, within Pf6N2E2_1188 at 1392.281 kb on - strand, within Pf6N2E2_1189 at 1392.281 kb on - strand, within Pf6N2E2_1189 at 1392.534 kb on + strand, within Pf6N2E2_1189 at 1392.535 kb on - strand, within Pf6N2E2_1189 at 1392.551 kb on - strand, within Pf6N2E2_1189 at 1392.551 kb on - strand, within Pf6N2E2_1189 at 1392.683 kb on + strand, within Pf6N2E2_1189 at 1392.684 kb on - strand, within Pf6N2E2_1189 at 1392.831 kb on + strand, within Pf6N2E2_1189 at 1393.047 kb on + strand, within Pf6N2E2_1189 at 1393.048 kb on - strand, within Pf6N2E2_1189 at 1393.056 kb on + strand, within Pf6N2E2_1189 at 1393.057 kb on - strand, within Pf6N2E2_1189 at 1393.110 kb on + strand, within Pf6N2E2_1189 at 1393.143 kb on + strand, within Pf6N2E2_1189 at 1393.190 kb on + strand at 1393.191 kb on - strand at 1393.191 kb on - strand at 1393.191 kb on - strand at 1393.191 kb on - strand at 1393.268 kb on + strand at 1393.372 kb on + strand at 1393.372 kb on + strand at 1393.372 kb on + strand at 1393.372 kb on + strand at 1393.372 kb on + strand at 1393.372 kb on + strand at 1393.372 kb on + strand at 1393.372 kb on + strand at 1393.372 kb on + strand at 1393.372 kb on + strand at 1393.372 kb on + strand at 1393.373 kb on - strand at 1393.373 kb on - strand at 1393.373 kb on - strand at 1393.373 kb on - strand at 1393.393 kb on + strand at 1393.394 kb on - strand at 1393.468 kb on - strand, within Pf6N2E2_1190 at 1393.468 kb on - strand, within Pf6N2E2_1190 at 1393.486 kb on + strand, within Pf6N2E2_1190 at 1393.501 kb on + strand, within Pf6N2E2_1190 at 1393.501 kb on + strand, within Pf6N2E2_1190 at 1393.502 kb on - strand, within Pf6N2E2_1190 at 1393.502 kb on - strand, within Pf6N2E2_1190 at 1393.579 kb on + strand, within Pf6N2E2_1190 at 1393.579 kb on + strand, within Pf6N2E2_1190 at 1393.647 kb on + strand, within Pf6N2E2_1190 at 1393.666 kb on + strand, within Pf6N2E2_1190 at 1393.667 kb on - strand, within Pf6N2E2_1190 at 1393.667 kb on - strand, within Pf6N2E2_1190 at 1393.690 kb on + strand, within Pf6N2E2_1190 at 1393.691 kb on - strand, within Pf6N2E2_1190 at 1393.691 kb on - strand, within Pf6N2E2_1190 at 1393.828 kb on + strand, within Pf6N2E2_1190 at 1393.835 kb on - strand, within Pf6N2E2_1190 at 1393.844 kb on - strand, within Pf6N2E2_1190 at 1393.959 kb on + strand, within Pf6N2E2_1190 at 1393.960 kb on - strand, within Pf6N2E2_1190 at 1394.077 kb on + strand, within Pf6N2E2_1190 at 1394.080 kb on + strand, within Pf6N2E2_1190 at 1394.081 kb on - strand, within Pf6N2E2_1190 at 1394.086 kb on + strand, within Pf6N2E2_1190 at 1394.137 kb on + strand, within Pf6N2E2_1190 at 1394.150 kb on - strand, within Pf6N2E2_1190 at 1394.150 kb on - strand, within Pf6N2E2_1190
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N) remove 1,391,135 + Pf6N2E2_1187 0.89 -3.2 1,391,135 + Pf6N2E2_1187 0.89 -2.8 1,391,171 - -0.5 1,391,234 + -2.8 1,391,239 + -3.1 1,391,239 + -3.1 1,391,273 + -3.8 1,391,273 + -2.1 1,391,274 - -3.2 1,391,276 + -1.1 1,391,276 + -0.1 1,391,276 + -2.8 1,391,276 + -4.0 1,391,277 - -1.8 1,391,277 - +1.9 1,391,360 + -3.3 1,391,496 + Pf6N2E2_1188 0.25 -1.3 1,391,524 + Pf6N2E2_1188 0.28 -1.6 1,391,525 - Pf6N2E2_1188 0.28 -1.8 1,391,525 - Pf6N2E2_1188 0.28 -2.5 1,391,558 - Pf6N2E2_1188 0.32 -0.8 1,391,803 - Pf6N2E2_1188 0.61 -1.1 1,391,863 + Pf6N2E2_1188 0.68 -0.8 1,392,004 + Pf6N2E2_1188 0.84 +0.1 1,392,004 + Pf6N2E2_1188 0.84 -3.0 1,392,005 - Pf6N2E2_1188 0.84 -3.3 1,392,005 - Pf6N2E2_1188 0.84 -2.1 1,392,043 - Pf6N2E2_1188 0.89 -1.2 1,392,281 - Pf6N2E2_1189 0.13 +0.4 1,392,281 - Pf6N2E2_1189 0.13 -1.7 1,392,534 + Pf6N2E2_1189 0.34 -2.5 1,392,535 - Pf6N2E2_1189 0.35 -0.5 1,392,551 - Pf6N2E2_1189 0.36 -2.4 1,392,551 - Pf6N2E2_1189 0.36 -2.9 1,392,683 + Pf6N2E2_1189 0.47 -1.1 1,392,684 - Pf6N2E2_1189 0.47 -1.9 1,392,831 + Pf6N2E2_1189 0.60 -2.1 1,393,047 + Pf6N2E2_1189 0.78 -1.4 1,393,048 - Pf6N2E2_1189 0.78 -0.8 1,393,056 + Pf6N2E2_1189 0.79 -1.1 1,393,057 - Pf6N2E2_1189 0.79 -0.4 1,393,110 + Pf6N2E2_1189 0.84 -3.1 1,393,143 + Pf6N2E2_1189 0.86 -1.4 1,393,190 + -2.6 1,393,191 - +0.2 1,393,191 - -0.9 1,393,191 - -2.1 1,393,191 - -1.4 1,393,268 + -2.2 1,393,372 + -1.6 1,393,372 + -2.4 1,393,372 + -2.9 1,393,372 + -1.8 1,393,372 + -0.1 1,393,372 + -0.8 1,393,372 + -1.9 1,393,372 + +0.2 1,393,372 + -3.2 1,393,372 + -2.6 1,393,372 + -1.6 1,393,373 - +1.2 1,393,373 - +2.0 1,393,373 - +0.5 1,393,373 - +1.2 1,393,393 + -1.5 1,393,394 - -0.6 1,393,468 - Pf6N2E2_1190 0.17 +1.2 1,393,468 - Pf6N2E2_1190 0.17 +0.6 1,393,486 + Pf6N2E2_1190 0.18 -1.1 1,393,501 + Pf6N2E2_1190 0.20 -2.4 1,393,501 + Pf6N2E2_1190 0.20 -1.1 1,393,502 - Pf6N2E2_1190 0.20 -1.8 1,393,502 - Pf6N2E2_1190 0.20 -0.8 1,393,579 + Pf6N2E2_1190 0.28 -1.1 1,393,579 + Pf6N2E2_1190 0.28 -3.2 1,393,647 + Pf6N2E2_1190 0.34 -1.4 1,393,666 + Pf6N2E2_1190 0.36 -1.8 1,393,667 - Pf6N2E2_1190 0.36 -1.6 1,393,667 - Pf6N2E2_1190 0.36 -1.1 1,393,690 + Pf6N2E2_1190 0.39 -2.0 1,393,691 - Pf6N2E2_1190 0.39 -1.7 1,393,691 - Pf6N2E2_1190 0.39 +1.2 1,393,828 + Pf6N2E2_1190 0.52 -2.4 1,393,835 - Pf6N2E2_1190 0.53 -3.1 1,393,844 - Pf6N2E2_1190 0.54 -1.8 1,393,959 + Pf6N2E2_1190 0.66 +0.2 1,393,960 - Pf6N2E2_1190 0.66 -2.1 1,394,077 + Pf6N2E2_1190 0.77 -1.9 1,394,080 + Pf6N2E2_1190 0.78 -2.0 1,394,081 - Pf6N2E2_1190 0.78 -1.4 1,394,086 + Pf6N2E2_1190 0.78 -1.7 1,394,137 + Pf6N2E2_1190 0.83 +0.2 1,394,150 - Pf6N2E2_1190 0.85 -1.9 1,394,150 - Pf6N2E2_1190 0.85 -0.8
Or see this region's nucleotide sequence