Experiment: L-Aspartic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5804 and Pf6N2E2_5805 overlap by 4 nucleotides Pf6N2E2_5805 and Pf6N2E2_5806 are separated by 45 nucleotides Pf6N2E2_5806 and Pf6N2E2_5807 are separated by 191 nucleotides Pf6N2E2_5807 and Pf6N2E2_5808 are separated by 96 nucleotides
Pf6N2E2_5804 - 2-oxo-4-hydroxy-4-carboxy--5-ureidoimidazoline (OHCU) decarboxylase, at 6,621,711 to 6,622,226
_5804
Pf6N2E2_5805 - Uricase (urate oxidase) (EC 1.7.3.3), at 6,622,223 to 6,623,164
_5805
Pf6N2E2_5806 - hypothetical protein, at 6,623,210 to 6,623,386
_5806
Pf6N2E2_5807 - 5-Hydroxyisourate Hydrolase (HIUase) (EC 3.5.2.17), at 6,623,578 to 6,623,931
_5807
Pf6N2E2_5808 - hypothetical protein, at 6,624,028 to 6,625,617
_5808
Position (kb)
6623
6624 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6622.257 kb on + strand at 6622.288 kb on + strand at 6622.332 kb on + strand, within Pf6N2E2_5805 at 6622.332 kb on + strand, within Pf6N2E2_5805 at 6622.333 kb on - strand, within Pf6N2E2_5805 at 6622.435 kb on - strand, within Pf6N2E2_5805 at 6622.496 kb on + strand, within Pf6N2E2_5805 at 6622.497 kb on - strand, within Pf6N2E2_5805 at 6622.497 kb on - strand, within Pf6N2E2_5805 at 6622.522 kb on + strand, within Pf6N2E2_5805 at 6622.582 kb on - strand, within Pf6N2E2_5805 at 6622.599 kb on + strand, within Pf6N2E2_5805 at 6622.600 kb on - strand, within Pf6N2E2_5805 at 6622.600 kb on - strand, within Pf6N2E2_5805 at 6622.600 kb on - strand, within Pf6N2E2_5805 at 6622.600 kb on - strand, within Pf6N2E2_5805 at 6622.615 kb on - strand, within Pf6N2E2_5805 at 6622.615 kb on - strand, within Pf6N2E2_5805 at 6622.674 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.761 kb on + strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6622.762 kb on - strand, within Pf6N2E2_5805 at 6623.218 kb on + strand at 6623.218 kb on + strand at 6623.218 kb on + strand at 6623.219 kb on - strand at 6623.272 kb on - strand, within Pf6N2E2_5806 at 6623.316 kb on - strand, within Pf6N2E2_5806 at 6623.316 kb on - strand, within Pf6N2E2_5806 at 6623.348 kb on - strand, within Pf6N2E2_5806 at 6623.367 kb on + strand, within Pf6N2E2_5806 at 6623.384 kb on + strand at 6623.428 kb on - strand at 6623.592 kb on + strand at 6623.592 kb on + strand at 6623.593 kb on - strand at 6623.650 kb on + strand, within Pf6N2E2_5807 at 6623.740 kb on - strand, within Pf6N2E2_5807 at 6623.743 kb on + strand, within Pf6N2E2_5807 at 6623.743 kb on + strand, within Pf6N2E2_5807 at 6623.743 kb on + strand, within Pf6N2E2_5807 at 6623.744 kb on - strand, within Pf6N2E2_5807 at 6623.759 kb on - strand, within Pf6N2E2_5807 at 6623.786 kb on - strand, within Pf6N2E2_5807 at 6623.875 kb on + strand, within Pf6N2E2_5807 at 6623.876 kb on - strand, within Pf6N2E2_5807 at 6623.902 kb on + strand at 6623.903 kb on - strand at 6623.909 kb on - strand at 6623.917 kb on + strand at 6623.917 kb on + strand at 6623.918 kb on - strand at 6623.918 kb on - strand at 6623.918 kb on - strand at 6623.918 kb on - strand at 6624.025 kb on + strand at 6624.029 kb on + strand at 6624.030 kb on - strand at 6624.030 kb on - strand at 6624.030 kb on - strand at 6624.192 kb on + strand, within Pf6N2E2_5808 at 6624.193 kb on - strand, within Pf6N2E2_5808 at 6624.193 kb on - strand, within Pf6N2E2_5808 at 6624.206 kb on + strand, within Pf6N2E2_5808 at 6624.207 kb on - strand, within Pf6N2E2_5808 at 6624.207 kb on - strand, within Pf6N2E2_5808 at 6624.259 kb on + strand, within Pf6N2E2_5808 at 6624.260 kb on - strand, within Pf6N2E2_5808 at 6624.260 kb on - strand, within Pf6N2E2_5808 at 6624.260 kb on - strand, within Pf6N2E2_5808 at 6624.260 kb on - strand, within Pf6N2E2_5808 at 6624.260 kb on - strand, within Pf6N2E2_5808 at 6624.287 kb on - strand, within Pf6N2E2_5808 at 6624.287 kb on - strand, within Pf6N2E2_5808 at 6624.303 kb on + strand, within Pf6N2E2_5808 at 6624.303 kb on + strand, within Pf6N2E2_5808 at 6624.303 kb on + strand, within Pf6N2E2_5808 at 6624.303 kb on + strand, within Pf6N2E2_5808 at 6624.304 kb on - strand, within Pf6N2E2_5808 at 6624.304 kb on - strand, within Pf6N2E2_5808 at 6624.304 kb on - strand, within Pf6N2E2_5808 at 6624.304 kb on - strand, within Pf6N2E2_5808 at 6624.304 kb on - strand, within Pf6N2E2_5808 at 6624.304 kb on - strand, within Pf6N2E2_5808 at 6624.304 kb on - strand at 6624.304 kb on - strand, within Pf6N2E2_5808 at 6624.308 kb on + strand, within Pf6N2E2_5808 at 6624.309 kb on - strand, within Pf6N2E2_5808 at 6624.309 kb on - strand, within Pf6N2E2_5808 at 6624.360 kb on + strand, within Pf6N2E2_5808 at 6624.386 kb on + strand, within Pf6N2E2_5808 at 6624.386 kb on + strand, within Pf6N2E2_5808 at 6624.386 kb on + strand, within Pf6N2E2_5808 at 6624.386 kb on + strand, within Pf6N2E2_5808 at 6624.386 kb on + strand, within Pf6N2E2_5808
Per-strain Table
Position Strand Gene LocusTag Fraction L-Aspartic Acid (N) remove 6,622,257 + -1.1 6,622,288 + +0.2 6,622,332 + Pf6N2E2_5805 0.12 -0.5 6,622,332 + Pf6N2E2_5805 0.12 +1.4 6,622,333 - Pf6N2E2_5805 0.12 -1.8 6,622,435 - Pf6N2E2_5805 0.23 +1.1 6,622,496 + Pf6N2E2_5805 0.29 -0.9 6,622,497 - Pf6N2E2_5805 0.29 -1.5 6,622,497 - Pf6N2E2_5805 0.29 +1.8 6,622,522 + Pf6N2E2_5805 0.32 -1.6 6,622,582 - Pf6N2E2_5805 0.38 +0.4 6,622,599 + Pf6N2E2_5805 0.40 -0.2 6,622,600 - Pf6N2E2_5805 0.40 +0.6 6,622,600 - Pf6N2E2_5805 0.40 +0.4 6,622,600 - Pf6N2E2_5805 0.40 +0.3 6,622,600 - Pf6N2E2_5805 0.40 -0.8 6,622,615 - Pf6N2E2_5805 0.42 +0.2 6,622,615 - Pf6N2E2_5805 0.42 +0.1 6,622,674 + Pf6N2E2_5805 0.48 -0.0 6,622,761 + Pf6N2E2_5805 0.57 -1.1 6,622,761 + Pf6N2E2_5805 0.57 +0.6 6,622,761 + Pf6N2E2_5805 0.57 +0.3 6,622,761 + Pf6N2E2_5805 0.57 -0.9 6,622,761 + Pf6N2E2_5805 0.57 -1.0 6,622,761 + Pf6N2E2_5805 0.57 -2.3 6,622,761 + Pf6N2E2_5805 0.57 -0.7 6,622,761 + Pf6N2E2_5805 0.57 -1.1 6,622,761 + Pf6N2E2_5805 0.57 -0.4 6,622,762 - Pf6N2E2_5805 0.57 -0.9 6,622,762 - Pf6N2E2_5805 0.57 +0.3 6,622,762 - Pf6N2E2_5805 0.57 -1.4 6,622,762 - Pf6N2E2_5805 0.57 +1.2 6,622,762 - Pf6N2E2_5805 0.57 +1.6 6,623,218 + -0.8 6,623,218 + +0.3 6,623,218 + +1.8 6,623,219 - -0.7 6,623,272 - Pf6N2E2_5806 0.35 -2.1 6,623,316 - Pf6N2E2_5806 0.60 -0.1 6,623,316 - Pf6N2E2_5806 0.60 -0.8 6,623,348 - Pf6N2E2_5806 0.78 -1.9 6,623,367 + Pf6N2E2_5806 0.89 -1.1 6,623,384 + +0.6 6,623,428 - +1.5 6,623,592 + +1.3 6,623,592 + -0.5 6,623,593 - -2.2 6,623,650 + Pf6N2E2_5807 0.20 -0.2 6,623,740 - Pf6N2E2_5807 0.46 +2.5 6,623,743 + Pf6N2E2_5807 0.47 -1.3 6,623,743 + Pf6N2E2_5807 0.47 -0.6 6,623,743 + Pf6N2E2_5807 0.47 +1.0 6,623,744 - Pf6N2E2_5807 0.47 +0.8 6,623,759 - Pf6N2E2_5807 0.51 -3.1 6,623,786 - Pf6N2E2_5807 0.59 +1.2 6,623,875 + Pf6N2E2_5807 0.84 +0.2 6,623,876 - Pf6N2E2_5807 0.84 +1.2 6,623,902 + +0.2 6,623,903 - -1.4 6,623,909 - +0.8 6,623,917 + -0.4 6,623,917 + +0.6 6,623,918 - -0.1 6,623,918 - +0.6 6,623,918 - -0.5 6,623,918 - +0.1 6,624,025 + +0.4 6,624,029 + +0.2 6,624,030 - -0.1 6,624,030 - -0.4 6,624,030 - -0.3 6,624,192 + Pf6N2E2_5808 0.10 -0.5 6,624,193 - Pf6N2E2_5808 0.10 -0.2 6,624,193 - Pf6N2E2_5808 0.10 -0.2 6,624,206 + Pf6N2E2_5808 0.11 -0.8 6,624,207 - Pf6N2E2_5808 0.11 -0.4 6,624,207 - Pf6N2E2_5808 0.11 -1.3 6,624,259 + Pf6N2E2_5808 0.15 +0.1 6,624,260 - Pf6N2E2_5808 0.15 +1.5 6,624,260 - Pf6N2E2_5808 0.15 +1.8 6,624,260 - Pf6N2E2_5808 0.15 -1.2 6,624,260 - Pf6N2E2_5808 0.15 +0.4 6,624,260 - Pf6N2E2_5808 0.15 +1.2 6,624,287 - Pf6N2E2_5808 0.16 -0.6 6,624,287 - Pf6N2E2_5808 0.16 -0.5 6,624,303 + Pf6N2E2_5808 0.17 +0.8 6,624,303 + Pf6N2E2_5808 0.17 -0.5 6,624,303 + Pf6N2E2_5808 0.17 -1.0 6,624,303 + Pf6N2E2_5808 0.17 +1.6 6,624,304 - Pf6N2E2_5808 0.17 +0.0 6,624,304 - Pf6N2E2_5808 0.17 -0.3 6,624,304 - Pf6N2E2_5808 0.17 -2.1 6,624,304 - Pf6N2E2_5808 0.17 -0.8 6,624,304 - Pf6N2E2_5808 0.17 +0.7 6,624,304 - Pf6N2E2_5808 0.17 +0.1 6,624,304 - +1.8 6,624,304 - Pf6N2E2_5808 0.17 +0.4 6,624,308 + Pf6N2E2_5808 0.18 -2.9 6,624,309 - Pf6N2E2_5808 0.18 +0.7 6,624,309 - Pf6N2E2_5808 0.18 -0.9 6,624,360 + Pf6N2E2_5808 0.21 -1.2 6,624,386 + Pf6N2E2_5808 0.23 -1.0 6,624,386 + Pf6N2E2_5808 0.23 +0.2 6,624,386 + Pf6N2E2_5808 0.23 +1.4 6,624,386 + Pf6N2E2_5808 0.23 +0.5 6,624,386 + Pf6N2E2_5808 0.23 +0.3
Or see this region's nucleotide sequence