Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5103

Experiment: L-Aspartic Acid (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5102 and Pf6N2E2_5103 are separated by 267 nucleotidesPf6N2E2_5103 and Pf6N2E2_5104 are separated by 9 nucleotidesPf6N2E2_5104 and Pf6N2E2_5105 are separated by 80 nucleotides Pf6N2E2_5102 - GTP-binding protein Obg, at 5,883,505 to 5,884,728 _5102 Pf6N2E2_5103 - Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA, at 5,884,996 to 5,886,114 _5103 Pf6N2E2_5104 - Conserved uncharacterized protein CreA, at 5,886,124 to 5,886,579 _5104 Pf6N2E2_5105 - hypothetical protein, at 5,886,660 to 5,888,699 _5105 Position (kb) 5884 5885 5886 5887Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5884.714 kb on + strandat 5884.714 kb on + strandat 5884.715 kb on - strandat 5884.715 kb on - strandat 5884.762 kb on + strandat 5884.799 kb on + strandat 5884.799 kb on + strandat 5884.800 kb on - strandat 5884.800 kb on - strandat 5884.920 kb on + strandat 5884.937 kb on + strandat 5884.937 kb on + strandat 5884.937 kb on + strandat 5885.846 kb on + strand, within Pf6N2E2_5103at 5885.846 kb on + strand, within Pf6N2E2_5103at 5886.287 kb on - strand, within Pf6N2E2_5104at 5886.381 kb on + strand, within Pf6N2E2_5104at 5886.381 kb on + strand, within Pf6N2E2_5104at 5886.381 kb on + strand, within Pf6N2E2_5104at 5886.489 kb on + strand, within Pf6N2E2_5104at 5886.489 kb on + strand, within Pf6N2E2_5104at 5886.490 kb on - strand, within Pf6N2E2_5104at 5886.490 kb on - strand, within Pf6N2E2_5104at 5886.500 kb on - strand, within Pf6N2E2_5104at 5886.500 kb on - strand, within Pf6N2E2_5104at 5886.508 kb on + strand, within Pf6N2E2_5104at 5886.508 kb on + strand, within Pf6N2E2_5104at 5886.577 kb on + strandat 5886.578 kb on - strandat 5886.617 kb on + strandat 5886.621 kb on + strandat 5886.622 kb on - strandat 5886.659 kb on + strandat 5886.660 kb on - strandat 5886.660 kb on - strandat 5886.685 kb on + strandat 5886.730 kb on - strandat 5886.760 kb on + strandat 5886.797 kb on + strandat 5886.820 kb on + strandat 5886.820 kb on + strandat 5886.820 kb on + strandat 5886.826 kb on - strandat 5886.826 kb on - strandat 5886.876 kb on + strand, within Pf6N2E2_5105at 5886.876 kb on + strand, within Pf6N2E2_5105at 5886.876 kb on + strand, within Pf6N2E2_5105at 5886.877 kb on - strand, within Pf6N2E2_5105at 5886.906 kb on + strand, within Pf6N2E2_5105at 5886.906 kb on + strand, within Pf6N2E2_5105at 5886.907 kb on - strand, within Pf6N2E2_5105

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Aspartic Acid (N)
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5,884,714 + +0.3
5,884,714 + +1.7
5,884,715 - +0.6
5,884,715 - +0.6
5,884,762 + -0.0
5,884,799 + -0.7
5,884,799 + -0.3
5,884,800 - +0.6
5,884,800 - -2.2
5,884,920 + -0.3
5,884,937 + -0.4
5,884,937 + -0.9
5,884,937 + -0.6
5,885,846 + Pf6N2E2_5103 0.76 -1.7
5,885,846 + Pf6N2E2_5103 0.76 -1.0
5,886,287 - Pf6N2E2_5104 0.36 +1.3
5,886,381 + Pf6N2E2_5104 0.56 -0.2
5,886,381 + Pf6N2E2_5104 0.56 +1.1
5,886,381 + Pf6N2E2_5104 0.56 -2.1
5,886,489 + Pf6N2E2_5104 0.80 +0.8
5,886,489 + Pf6N2E2_5104 0.80 +0.4
5,886,490 - Pf6N2E2_5104 0.80 +0.6
5,886,490 - Pf6N2E2_5104 0.80 -1.6
5,886,500 - Pf6N2E2_5104 0.82 -0.2
5,886,500 - Pf6N2E2_5104 0.82 +0.6
5,886,508 + Pf6N2E2_5104 0.84 -0.3
5,886,508 + Pf6N2E2_5104 0.84 -0.0
5,886,577 + +0.5
5,886,578 - -1.3
5,886,617 + -0.5
5,886,621 + +1.4
5,886,622 - +0.1
5,886,659 + +1.2
5,886,660 - +1.7
5,886,660 - -0.0
5,886,685 + +0.3
5,886,730 - +0.5
5,886,760 + -0.3
5,886,797 + -0.2
5,886,820 + +0.6
5,886,820 + -1.2
5,886,820 + -1.4
5,886,826 - -1.3
5,886,826 - +1.6
5,886,876 + Pf6N2E2_5105 0.11 -3.0
5,886,876 + Pf6N2E2_5105 0.11 -0.2
5,886,876 + Pf6N2E2_5105 0.11 +1.0
5,886,877 - Pf6N2E2_5105 0.11 +1.8
5,886,906 + Pf6N2E2_5105 0.12 +0.0
5,886,906 + Pf6N2E2_5105 0.12 +0.1
5,886,907 - Pf6N2E2_5105 0.12 -0.7

Or see this region's nucleotide sequence