Experiment: L-Aspartic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4896 and Pf6N2E2_4897 are separated by 68 nucleotides Pf6N2E2_4897 and Pf6N2E2_4898 are separated by 3 nucleotides
Pf6N2E2_4896 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 5,649,475 to 5,650,470
_4896
Pf6N2E2_4897 - hypothetical protein, at 5,650,539 to 5,650,814
_4897
Pf6N2E2_4898 - Biotin carboxylase (EC 6.3.4.14), at 5,650,818 to 5,651,921
_4898
Position (kb)
5650
5651 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5650.001 kb on + strand, within Pf6N2E2_4896 at 5650.099 kb on - strand, within Pf6N2E2_4896 at 5650.224 kb on + strand, within Pf6N2E2_4896 at 5650.224 kb on + strand, within Pf6N2E2_4896 at 5650.225 kb on - strand, within Pf6N2E2_4896 at 5650.225 kb on - strand, within Pf6N2E2_4896 at 5650.225 kb on - strand, within Pf6N2E2_4896 at 5650.225 kb on - strand, within Pf6N2E2_4896 at 5650.273 kb on + strand, within Pf6N2E2_4896 at 5650.274 kb on - strand, within Pf6N2E2_4896 at 5650.274 kb on - strand, within Pf6N2E2_4896 at 5650.274 kb on - strand, within Pf6N2E2_4896 at 5650.442 kb on + strand at 5650.443 kb on - strand at 5650.540 kb on + strand at 5650.540 kb on + strand at 5650.540 kb on + strand at 5650.541 kb on - strand at 5650.541 kb on - strand at 5650.552 kb on - strand at 5650.552 kb on - strand at 5650.653 kb on + strand, within Pf6N2E2_4897 at 5650.653 kb on + strand, within Pf6N2E2_4897 at 5650.689 kb on + strand, within Pf6N2E2_4897 at 5650.689 kb on + strand, within Pf6N2E2_4897 at 5650.703 kb on + strand, within Pf6N2E2_4897 at 5650.704 kb on - strand, within Pf6N2E2_4897 at 5650.704 kb on - strand, within Pf6N2E2_4897 at 5650.854 kb on + strand at 5650.942 kb on - strand, within Pf6N2E2_4898 at 5650.981 kb on - strand, within Pf6N2E2_4898 at 5650.981 kb on - strand, within Pf6N2E2_4898 at 5650.981 kb on - strand, within Pf6N2E2_4898 at 5650.981 kb on - strand, within Pf6N2E2_4898 at 5651.065 kb on - strand, within Pf6N2E2_4898 at 5651.065 kb on - strand, within Pf6N2E2_4898 at 5651.065 kb on - strand, within Pf6N2E2_4898 at 5651.065 kb on - strand, within Pf6N2E2_4898 at 5651.065 kb on - strand, within Pf6N2E2_4898 at 5651.118 kb on - strand, within Pf6N2E2_4898 at 5651.241 kb on + strand, within Pf6N2E2_4898 at 5651.241 kb on + strand, within Pf6N2E2_4898 at 5651.241 kb on + strand, within Pf6N2E2_4898 at 5651.242 kb on - strand, within Pf6N2E2_4898 at 5651.242 kb on - strand, within Pf6N2E2_4898 at 5651.448 kb on + strand, within Pf6N2E2_4898 at 5651.448 kb on + strand, within Pf6N2E2_4898 at 5651.449 kb on - strand, within Pf6N2E2_4898 at 5651.449 kb on - strand, within Pf6N2E2_4898 at 5651.449 kb on - strand, within Pf6N2E2_4898 at 5651.496 kb on + strand, within Pf6N2E2_4898 at 5651.496 kb on + strand, within Pf6N2E2_4898 at 5651.496 kb on + strand, within Pf6N2E2_4898 at 5651.497 kb on - strand, within Pf6N2E2_4898 at 5651.518 kb on + strand, within Pf6N2E2_4898 at 5651.518 kb on + strand, within Pf6N2E2_4898 at 5651.518 kb on + strand, within Pf6N2E2_4898 at 5651.518 kb on + strand, within Pf6N2E2_4898 at 5651.519 kb on - strand, within Pf6N2E2_4898 at 5651.521 kb on + strand, within Pf6N2E2_4898 at 5651.521 kb on + strand, within Pf6N2E2_4898 at 5651.521 kb on + strand, within Pf6N2E2_4898 at 5651.522 kb on - strand, within Pf6N2E2_4898 at 5651.522 kb on - strand, within Pf6N2E2_4898 at 5651.532 kb on + strand, within Pf6N2E2_4898 at 5651.533 kb on - strand, within Pf6N2E2_4898 at 5651.533 kb on - strand, within Pf6N2E2_4898 at 5651.534 kb on + strand, within Pf6N2E2_4898 at 5651.534 kb on + strand, within Pf6N2E2_4898 at 5651.535 kb on - strand, within Pf6N2E2_4898 at 5651.622 kb on + strand, within Pf6N2E2_4898 at 5651.653 kb on - strand, within Pf6N2E2_4898 at 5651.653 kb on - strand, within Pf6N2E2_4898 at 5651.814 kb on + strand at 5651.814 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Aspartic Acid (N) remove 5,650,001 + Pf6N2E2_4896 0.53 +0.1 5,650,099 - Pf6N2E2_4896 0.63 +0.2 5,650,224 + Pf6N2E2_4896 0.75 -0.1 5,650,224 + Pf6N2E2_4896 0.75 -0.5 5,650,225 - Pf6N2E2_4896 0.75 -0.5 5,650,225 - Pf6N2E2_4896 0.75 -0.4 5,650,225 - Pf6N2E2_4896 0.75 -0.1 5,650,225 - Pf6N2E2_4896 0.75 -0.2 5,650,273 + Pf6N2E2_4896 0.80 +1.0 5,650,274 - Pf6N2E2_4896 0.80 +0.6 5,650,274 - Pf6N2E2_4896 0.80 +0.2 5,650,274 - Pf6N2E2_4896 0.80 -0.5 5,650,442 + +0.4 5,650,443 - -1.1 5,650,540 + -0.4 5,650,540 + +1.4 5,650,540 + -0.7 5,650,541 - +0.3 5,650,541 - +0.6 5,650,552 - +1.0 5,650,552 - +0.3 5,650,653 + Pf6N2E2_4897 0.41 -0.7 5,650,653 + Pf6N2E2_4897 0.41 -2.4 5,650,689 + Pf6N2E2_4897 0.54 -0.0 5,650,689 + Pf6N2E2_4897 0.54 -0.0 5,650,703 + Pf6N2E2_4897 0.59 +0.4 5,650,704 - Pf6N2E2_4897 0.60 -0.2 5,650,704 - Pf6N2E2_4897 0.60 +0.0 5,650,854 + +0.4 5,650,942 - Pf6N2E2_4898 0.11 +0.3 5,650,981 - Pf6N2E2_4898 0.15 -0.3 5,650,981 - Pf6N2E2_4898 0.15 +0.9 5,650,981 - Pf6N2E2_4898 0.15 -0.1 5,650,981 - Pf6N2E2_4898 0.15 -0.0 5,651,065 - Pf6N2E2_4898 0.22 +1.8 5,651,065 - Pf6N2E2_4898 0.22 -0.1 5,651,065 - Pf6N2E2_4898 0.22 +0.6 5,651,065 - Pf6N2E2_4898 0.22 -0.3 5,651,065 - Pf6N2E2_4898 0.22 -0.4 5,651,118 - Pf6N2E2_4898 0.27 +0.6 5,651,241 + Pf6N2E2_4898 0.38 +0.5 5,651,241 + Pf6N2E2_4898 0.38 +0.0 5,651,241 + Pf6N2E2_4898 0.38 -0.1 5,651,242 - Pf6N2E2_4898 0.38 -0.2 5,651,242 - Pf6N2E2_4898 0.38 -0.2 5,651,448 + Pf6N2E2_4898 0.57 -0.5 5,651,448 + Pf6N2E2_4898 0.57 -1.2 5,651,449 - Pf6N2E2_4898 0.57 +0.4 5,651,449 - Pf6N2E2_4898 0.57 -0.2 5,651,449 - Pf6N2E2_4898 0.57 +1.0 5,651,496 + Pf6N2E2_4898 0.61 +0.2 5,651,496 + Pf6N2E2_4898 0.61 +1.1 5,651,496 + Pf6N2E2_4898 0.61 +0.8 5,651,497 - Pf6N2E2_4898 0.62 -0.5 5,651,518 + Pf6N2E2_4898 0.63 +0.5 5,651,518 + Pf6N2E2_4898 0.63 -0.4 5,651,518 + Pf6N2E2_4898 0.63 -0.1 5,651,518 + Pf6N2E2_4898 0.63 +0.7 5,651,519 - Pf6N2E2_4898 0.63 -1.1 5,651,521 + Pf6N2E2_4898 0.64 -0.6 5,651,521 + Pf6N2E2_4898 0.64 +0.4 5,651,521 + Pf6N2E2_4898 0.64 +1.5 5,651,522 - Pf6N2E2_4898 0.64 +0.6 5,651,522 - Pf6N2E2_4898 0.64 +0.8 5,651,532 + Pf6N2E2_4898 0.65 -2.2 5,651,533 - Pf6N2E2_4898 0.65 -1.2 5,651,533 - Pf6N2E2_4898 0.65 -0.8 5,651,534 + Pf6N2E2_4898 0.65 +0.2 5,651,534 + Pf6N2E2_4898 0.65 +1.1 5,651,535 - Pf6N2E2_4898 0.65 +1.6 5,651,622 + Pf6N2E2_4898 0.73 -0.7 5,651,653 - Pf6N2E2_4898 0.76 -0.7 5,651,653 - Pf6N2E2_4898 0.76 -0.6 5,651,814 + +1.0 5,651,814 + +0.4
Or see this region's nucleotide sequence