Experiment: L-Aspartic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4082 and Pf6N2E2_4083 are separated by 9 nucleotides Pf6N2E2_4083 and Pf6N2E2_4084 are separated by 385 nucleotides Pf6N2E2_4084 and Pf6N2E2_4085 are separated by 167 nucleotides
Pf6N2E2_4082 - Phosphoribosylaminoimidazole carboxylase catalytic subunit (EC 4.1.1.21), at 4,758,994 to 4,759,416
_4082
Pf6N2E2_4083 - hypothetical protein, at 4,759,426 to 4,759,626
_4083
Pf6N2E2_4084 - Transcriptional regulator, at 4,760,012 to 4,760,929
_4084
Pf6N2E2_4085 - Aspartate ammonia-lyase (EC 4.3.1.1), at 4,761,097 to 4,762,533
_4085
Position (kb)
4760
4761 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4759.566 kb on - strand, within Pf6N2E2_4083 at 4759.606 kb on - strand, within Pf6N2E2_4083 at 4759.606 kb on - strand, within Pf6N2E2_4083 at 4759.606 kb on - strand, within Pf6N2E2_4083 at 4759.606 kb on - strand, within Pf6N2E2_4083 at 4759.606 kb on - strand, within Pf6N2E2_4083 at 4759.673 kb on + strand at 4759.674 kb on - strand at 4759.905 kb on + strand at 4759.905 kb on + strand at 4759.905 kb on + strand at 4760.256 kb on + strand, within Pf6N2E2_4084 at 4760.257 kb on - strand at 4760.257 kb on - strand, within Pf6N2E2_4084 at 4760.332 kb on - strand, within Pf6N2E2_4084 at 4760.481 kb on + strand, within Pf6N2E2_4084 at 4760.481 kb on + strand, within Pf6N2E2_4084 at 4760.482 kb on - strand, within Pf6N2E2_4084 at 4760.482 kb on - strand, within Pf6N2E2_4084 at 4760.482 kb on - strand, within Pf6N2E2_4084 at 4760.645 kb on + strand, within Pf6N2E2_4084 at 4760.645 kb on + strand, within Pf6N2E2_4084 at 4760.645 kb on + strand, within Pf6N2E2_4084 at 4760.645 kb on + strand, within Pf6N2E2_4084 at 4760.645 kb on + strand, within Pf6N2E2_4084 at 4760.646 kb on - strand, within Pf6N2E2_4084 at 4760.646 kb on - strand, within Pf6N2E2_4084 at 4760.646 kb on - strand, within Pf6N2E2_4084 at 4760.646 kb on - strand, within Pf6N2E2_4084 at 4761.039 kb on - strand at 4761.179 kb on - strand at 4761.502 kb on + strand, within Pf6N2E2_4085
Per-strain Table
Position Strand Gene LocusTag Fraction L-Aspartic Acid (N) remove 4,759,566 - Pf6N2E2_4083 0.70 -0.6 4,759,606 - Pf6N2E2_4083 0.90 -0.8 4,759,606 - Pf6N2E2_4083 0.90 -1.2 4,759,606 - Pf6N2E2_4083 0.90 -0.3 4,759,606 - Pf6N2E2_4083 0.90 -0.3 4,759,606 - Pf6N2E2_4083 0.90 -1.7 4,759,673 + -3.0 4,759,674 - +0.3 4,759,905 + +0.5 4,759,905 + +0.3 4,759,905 + -0.2 4,760,256 + Pf6N2E2_4084 0.27 -0.3 4,760,257 - +0.9 4,760,257 - Pf6N2E2_4084 0.27 -1.5 4,760,332 - Pf6N2E2_4084 0.35 -1.5 4,760,481 + Pf6N2E2_4084 0.51 -0.2 4,760,481 + Pf6N2E2_4084 0.51 -0.8 4,760,482 - Pf6N2E2_4084 0.51 +2.1 4,760,482 - Pf6N2E2_4084 0.51 -0.8 4,760,482 - Pf6N2E2_4084 0.51 -1.0 4,760,645 + Pf6N2E2_4084 0.69 -0.1 4,760,645 + Pf6N2E2_4084 0.69 -1.7 4,760,645 + Pf6N2E2_4084 0.69 -2.4 4,760,645 + Pf6N2E2_4084 0.69 -0.9 4,760,645 + Pf6N2E2_4084 0.69 -0.7 4,760,646 - Pf6N2E2_4084 0.69 -0.1 4,760,646 - Pf6N2E2_4084 0.69 -0.6 4,760,646 - Pf6N2E2_4084 0.69 -2.1 4,760,646 - Pf6N2E2_4084 0.69 -1.7 4,761,039 - -1.1 4,761,179 - -2.4 4,761,502 + Pf6N2E2_4085 0.28 -0.5
Or see this region's nucleotide sequence