Experiment: L-Aspartic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3370 and Pf6N2E2_3371 are separated by 77 nucleotides Pf6N2E2_3371 and Pf6N2E2_3372 are separated by 163 nucleotides Pf6N2E2_3372 and Pf6N2E2_3373 overlap by 33 nucleotides Pf6N2E2_3373 and Pf6N2E2_3374 are separated by 59 nucleotides Pf6N2E2_3374 and Pf6N2E2_3375 are separated by 11 nucleotides Pf6N2E2_3375 and Pf6N2E2_3376 are separated by 7 nucleotides
Pf6N2E2_3370 - DNA repair protein RecN, at 3,903,544 to 3,905,217
_3370
Pf6N2E2_3371 - Ferric uptake regulation protein FUR, at 3,905,295 to 3,905,699
_3371
Pf6N2E2_3372 - hypothetical protein, at 3,905,863 to 3,906,003
_3372
Pf6N2E2_3373 - Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex, at 3,905,971 to 3,906,324
_3373
Pf6N2E2_3374 - UPF0125 protein yfjF, at 3,906,384 to 3,906,671
_3374
Pf6N2E2_3375 - Putative oligoketide cyclase/lipid transport protein, similarity with yeast ubiquinone-binding protein YOL008W, at 3,906,683 to 3,907,126
_3375
Pf6N2E2_3376 - Sodium-dependent transporter family protein, at 3,907,134 to 3,908,426
_3376
Position (kb)
3905
3906
3907 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3905.003 kb on + strand, within Pf6N2E2_3370 at 3905.003 kb on + strand, within Pf6N2E2_3370 at 3905.003 kb on + strand, within Pf6N2E2_3370 at 3905.004 kb on - strand, within Pf6N2E2_3370 at 3905.004 kb on - strand, within Pf6N2E2_3370 at 3905.004 kb on - strand, within Pf6N2E2_3370 at 3905.004 kb on - strand, within Pf6N2E2_3370 at 3905.054 kb on + strand at 3905.054 kb on + strand at 3905.054 kb on + strand at 3905.054 kb on + strand at 3905.054 kb on + strand at 3905.054 kb on + strand at 3905.054 kb on + strand at 3905.054 kb on + strand at 3905.054 kb on + strand at 3905.054 kb on + strand at 3905.054 kb on + strand at 3905.054 kb on + strand at 3905.055 kb on - strand at 3905.055 kb on - strand at 3905.055 kb on - strand at 3905.055 kb on - strand at 3905.055 kb on - strand at 3905.055 kb on - strand at 3905.055 kb on - strand at 3905.055 kb on - strand at 3905.055 kb on - strand at 3905.055 kb on - strand at 3905.064 kb on + strand at 3905.064 kb on + strand at 3905.064 kb on + strand at 3905.064 kb on + strand at 3905.064 kb on + strand at 3905.064 kb on + strand at 3905.065 kb on - strand at 3905.065 kb on - strand at 3905.065 kb on - strand at 3905.069 kb on + strand at 3905.069 kb on + strand at 3905.070 kb on - strand at 3905.070 kb on - strand at 3905.126 kb on + strand at 3905.126 kb on + strand at 3905.126 kb on + strand at 3905.276 kb on - strand at 3905.278 kb on + strand at 3905.279 kb on - strand at 3905.279 kb on - strand at 3905.279 kb on - strand at 3905.279 kb on - strand at 3905.296 kb on + strand at 3905.296 kb on + strand at 3905.297 kb on - strand at 3906.131 kb on + strand, within Pf6N2E2_3373 at 3906.131 kb on + strand, within Pf6N2E2_3373 at 3906.282 kb on - strand, within Pf6N2E2_3373 at 3906.310 kb on - strand at 3906.310 kb on - strand at 3906.322 kb on + strand at 3906.322 kb on + strand at 3906.322 kb on + strand at 3906.322 kb on + strand at 3906.323 kb on - strand at 3906.323 kb on - strand at 3906.323 kb on - strand at 3906.362 kb on - strand at 3906.362 kb on - strand at 3906.385 kb on + strand at 3906.676 kb on - strand at 3906.676 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.162 kb on - strand at 3907.162 kb on - strand at 3907.162 kb on - strand at 3907.162 kb on - strand at 3907.187 kb on - strand at 3907.187 kb on - strand at 3907.187 kb on - strand at 3907.187 kb on - strand at 3907.187 kb on - strand at 3907.187 kb on - strand at 3907.219 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Aspartic Acid (N) remove 3,905,003 + Pf6N2E2_3370 0.87 -0.5 3,905,003 + Pf6N2E2_3370 0.87 +1.0 3,905,003 + Pf6N2E2_3370 0.87 -0.5 3,905,004 - Pf6N2E2_3370 0.87 +0.8 3,905,004 - Pf6N2E2_3370 0.87 -0.4 3,905,004 - Pf6N2E2_3370 0.87 -0.3 3,905,004 - Pf6N2E2_3370 0.87 +0.3 3,905,054 + -0.3 3,905,054 + +0.6 3,905,054 + +1.8 3,905,054 + +1.5 3,905,054 + -0.3 3,905,054 + +1.0 3,905,054 + +0.9 3,905,054 + -3.0 3,905,054 + +0.1 3,905,054 + -0.1 3,905,054 + +0.9 3,905,054 + -0.5 3,905,055 - -0.7 3,905,055 - -0.7 3,905,055 - -0.8 3,905,055 - -1.0 3,905,055 - +0.5 3,905,055 - -0.4 3,905,055 - +0.1 3,905,055 - -2.0 3,905,055 - -0.5 3,905,055 - +0.7 3,905,064 + -0.9 3,905,064 + +1.1 3,905,064 + -1.1 3,905,064 + -1.4 3,905,064 + +0.4 3,905,064 + -0.0 3,905,065 - -0.0 3,905,065 - +0.7 3,905,065 - +0.3 3,905,069 + +0.5 3,905,069 + +0.6 3,905,070 - +0.5 3,905,070 - -0.9 3,905,126 + -2.6 3,905,126 + +0.7 3,905,126 + +0.7 3,905,276 - -1.8 3,905,278 + +0.8 3,905,279 - -2.3 3,905,279 - -0.3 3,905,279 - +0.3 3,905,279 - -1.0 3,905,296 + +0.1 3,905,296 + +1.3 3,905,297 - -0.1 3,906,131 + Pf6N2E2_3373 0.45 -2.4 3,906,131 + Pf6N2E2_3373 0.45 -0.5 3,906,282 - Pf6N2E2_3373 0.88 -1.9 3,906,310 - +0.9 3,906,310 - -0.3 3,906,322 + +0.4 3,906,322 + +0.6 3,906,322 + +0.3 3,906,322 + -1.1 3,906,323 - -1.7 3,906,323 - +1.0 3,906,323 - +0.2 3,906,362 - -0.8 3,906,362 - -0.2 3,906,385 + +0.9 3,906,676 - -0.9 3,906,676 - -2.3 3,907,129 - -0.3 3,907,129 - +0.9 3,907,129 - +1.0 3,907,129 - +1.0 3,907,129 - -0.2 3,907,129 - -1.3 3,907,129 - +0.2 3,907,129 - -2.0 3,907,129 - +0.7 3,907,162 - +1.7 3,907,162 - +2.1 3,907,162 - -0.5 3,907,162 - +0.2 3,907,187 - +0.8 3,907,187 - -0.5 3,907,187 - +0.3 3,907,187 - -0.5 3,907,187 - +0.3 3,907,187 - -1.0 3,907,219 - +0.0
Or see this region's nucleotide sequence