Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3185

Experiment: L-Aspartic Acid (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3182 and Pf6N2E2_3183 are separated by 88 nucleotidesPf6N2E2_3183 and Pf6N2E2_3184 are separated by 13 nucleotidesPf6N2E2_3184 and Pf6N2E2_3185 are separated by 3 nucleotidesPf6N2E2_3185 and Pf6N2E2_3186 are separated by 50 nucleotidesPf6N2E2_3186 and Pf6N2E2_3187 are separated by 8 nucleotidesPf6N2E2_3187 and Pf6N2E2_3188 are separated by 24 nucleotides Pf6N2E2_3182 - Nucleoside diphosphate kinase (EC 2.7.4.6), at 3,678,532 to 3,678,957 _3182 Pf6N2E2_3183 - Believed to be involved in assembly of Fe-S clusters, at 3,679,046 to 3,679,246 _3183 Pf6N2E2_3184 - Ferredoxin, 2Fe-2S, at 3,679,260 to 3,679,601 _3184 Pf6N2E2_3185 - Chaperone protein HscA, at 3,679,605 to 3,681,467 _3185 Pf6N2E2_3186 - Chaperone protein HscB, at 3,681,518 to 3,682,039 _3186 Pf6N2E2_3187 - Iron binding protein IscA for iron-sulfur cluster assembly, at 3,682,048 to 3,682,371 _3187 Pf6N2E2_3188 - Iron-sulfur cluster assembly scaffold protein IscU, at 3,682,396 to 3,682,782 _3188 Position (kb) 3679 3680 3681 3682Strain fitness (log2 ratio) -2 -1 0 1at 3679.006 kb on - strandat 3679.006 kb on - strandat 3679.008 kb on - strandat 3679.224 kb on - strand, within Pf6N2E2_3183at 3679.224 kb on - strand, within Pf6N2E2_3183at 3679.224 kb on - strand, within Pf6N2E2_3183at 3679.224 kb on - strand, within Pf6N2E2_3183at 3679.239 kb on + strandat 3679.240 kb on - strandat 3679.240 kb on - strandat 3679.240 kb on - strandat 3679.240 kb on - strandat 3679.240 kb on - strandat 3679.240 kb on - strandat 3679.240 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Aspartic Acid (N)
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3,679,006 - +0.1
3,679,006 - -0.4
3,679,008 - -1.0
3,679,224 - Pf6N2E2_3183 0.89 -0.2
3,679,224 - Pf6N2E2_3183 0.89 -1.8
3,679,224 - Pf6N2E2_3183 0.89 -0.5
3,679,224 - Pf6N2E2_3183 0.89 -0.5
3,679,239 + -0.7
3,679,240 - +0.9
3,679,240 - +0.7
3,679,240 - +0.3
3,679,240 - +0.1
3,679,240 - +1.0
3,679,240 - -1.1
3,679,240 - -0.5

Or see this region's nucleotide sequence