Experiment: L-Aspartic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3182 and Pf6N2E2_3183 are separated by 88 nucleotides Pf6N2E2_3183 and Pf6N2E2_3184 are separated by 13 nucleotides Pf6N2E2_3184 and Pf6N2E2_3185 are separated by 3 nucleotides Pf6N2E2_3185 and Pf6N2E2_3186 are separated by 50 nucleotides Pf6N2E2_3186 and Pf6N2E2_3187 are separated by 8 nucleotides Pf6N2E2_3187 and Pf6N2E2_3188 are separated by 24 nucleotides
Pf6N2E2_3182 - Nucleoside diphosphate kinase (EC 2.7.4.6), at 3,678,532 to 3,678,957
_3182
Pf6N2E2_3183 - Believed to be involved in assembly of Fe-S clusters, at 3,679,046 to 3,679,246
_3183
Pf6N2E2_3184 - Ferredoxin, 2Fe-2S, at 3,679,260 to 3,679,601
_3184
Pf6N2E2_3185 - Chaperone protein HscA, at 3,679,605 to 3,681,467
_3185
Pf6N2E2_3186 - Chaperone protein HscB, at 3,681,518 to 3,682,039
_3186
Pf6N2E2_3187 - Iron binding protein IscA for iron-sulfur cluster assembly, at 3,682,048 to 3,682,371
_3187
Pf6N2E2_3188 - Iron-sulfur cluster assembly scaffold protein IscU, at 3,682,396 to 3,682,782
_3188
Position (kb)
3679
3680
3681
3682 Strain fitness (log2 ratio)
-2
-1
0
1 at 3679.006 kb on - strand at 3679.006 kb on - strand at 3679.008 kb on - strand at 3679.224 kb on - strand, within Pf6N2E2_3183 at 3679.224 kb on - strand, within Pf6N2E2_3183 at 3679.224 kb on - strand, within Pf6N2E2_3183 at 3679.224 kb on - strand, within Pf6N2E2_3183 at 3679.239 kb on + strand at 3679.240 kb on - strand at 3679.240 kb on - strand at 3679.240 kb on - strand at 3679.240 kb on - strand at 3679.240 kb on - strand at 3679.240 kb on - strand at 3679.240 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Aspartic Acid (N) remove 3,679,006 - +0.1 3,679,006 - -0.4 3,679,008 - -1.0 3,679,224 - Pf6N2E2_3183 0.89 -0.2 3,679,224 - Pf6N2E2_3183 0.89 -1.8 3,679,224 - Pf6N2E2_3183 0.89 -0.5 3,679,224 - Pf6N2E2_3183 0.89 -0.5 3,679,239 + -0.7 3,679,240 - +0.9 3,679,240 - +0.7 3,679,240 - +0.3 3,679,240 - +0.1 3,679,240 - +1.0 3,679,240 - -1.1 3,679,240 - -0.5
Or see this region's nucleotide sequence