Experiment: L-Histidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_612 and Pf6N2E2_613 are separated by 118 nucleotides Pf6N2E2_613 and Pf6N2E2_614 overlap by 14 nucleotides Pf6N2E2_614 and Pf6N2E2_615 are separated by 156 nucleotides Pf6N2E2_615 and Pf6N2E2_616 are separated by 173 nucleotides Pf6N2E2_616 and Pf6N2E2_617 are separated by 143 nucleotides
Pf6N2E2_612 - Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26) <small>(from data)</small>, at 695,832 to 697,412
_612
Pf6N2E2_613 - lipoprotein, putative, at 697,531 to 697,869
_613
Pf6N2E2_614 - 3-carboxymuconate cyclase, at 697,856 to 699,031
_614
Pf6N2E2_615 - FIG00957600: hypothetical protein, at 699,188 to 699,367
_615
Pf6N2E2_616 - hypothetical protein, at 699,541 to 699,666
_616
Pf6N2E2_617 - Probable glutathione S-transferase, at 699,810 to 700,412
_617
Position (kb) 697
698
699
700 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 696.858 kb on + strand, within Pf6N2E2_612 at 697.705 kb on - strand, within Pf6N2E2_613 at 697.985 kb on + strand, within Pf6N2E2_614 at 697.986 kb on - strand, within Pf6N2E2_614 at 697.986 kb on - strand, within Pf6N2E2_614 at 697.986 kb on - strand, within Pf6N2E2_614 at 697.986 kb on - strand, within Pf6N2E2_614 at 698.153 kb on + strand, within Pf6N2E2_614 at 698.153 kb on + strand, within Pf6N2E2_614 at 698.266 kb on + strand, within Pf6N2E2_614 at 698.266 kb on + strand, within Pf6N2E2_614 at 698.270 kb on + strand, within Pf6N2E2_614 at 698.270 kb on + strand, within Pf6N2E2_614 at 698.270 kb on + strand, within Pf6N2E2_614 at 698.271 kb on - strand, within Pf6N2E2_614 at 698.271 kb on - strand, within Pf6N2E2_614 at 698.438 kb on + strand, within Pf6N2E2_614 at 698.438 kb on + strand, within Pf6N2E2_614 at 698.438 kb on + strand, within Pf6N2E2_614 at 698.438 kb on + strand, within Pf6N2E2_614 at 698.439 kb on - strand, within Pf6N2E2_614 at 698.439 kb on - strand, within Pf6N2E2_614 at 698.439 kb on - strand, within Pf6N2E2_614 at 698.439 kb on - strand, within Pf6N2E2_614 at 698.439 kb on - strand, within Pf6N2E2_614 at 698.439 kb on - strand, within Pf6N2E2_614 at 698.472 kb on + strand, within Pf6N2E2_614 at 698.472 kb on + strand, within Pf6N2E2_614 at 698.472 kb on + strand, within Pf6N2E2_614 at 698.472 kb on + strand, within Pf6N2E2_614 at 698.472 kb on + strand, within Pf6N2E2_614 at 698.473 kb on - strand, within Pf6N2E2_614 at 698.473 kb on - strand, within Pf6N2E2_614 at 698.756 kb on + strand, within Pf6N2E2_614 at 698.756 kb on + strand, within Pf6N2E2_614 at 699.067 kb on + strand at 699.068 kb on - strand at 699.068 kb on - strand at 699.098 kb on - strand at 699.299 kb on + strand, within Pf6N2E2_615 at 699.299 kb on + strand, within Pf6N2E2_615 at 699.300 kb on - strand, within Pf6N2E2_615 at 699.311 kb on + strand, within Pf6N2E2_615 at 699.311 kb on + strand, within Pf6N2E2_615 at 699.312 kb on - strand, within Pf6N2E2_615 at 699.335 kb on + strand, within Pf6N2E2_615 at 699.527 kb on + strand at 699.527 kb on + strand at 699.528 kb on - strand at 699.578 kb on - strand, within Pf6N2E2_616 at 699.812 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (N) remove 696,858 + Pf6N2E2_612 0.65 +0.5 697,705 - Pf6N2E2_613 0.51 -0.7 697,985 + Pf6N2E2_614 0.11 -1.7 697,986 - Pf6N2E2_614 0.11 -0.9 697,986 - Pf6N2E2_614 0.11 -0.1 697,986 - Pf6N2E2_614 0.11 -0.4 697,986 - Pf6N2E2_614 0.11 -1.0 698,153 + Pf6N2E2_614 0.25 -0.6 698,153 + Pf6N2E2_614 0.25 -0.4 698,266 + Pf6N2E2_614 0.35 +0.1 698,266 + Pf6N2E2_614 0.35 -1.4 698,270 + Pf6N2E2_614 0.35 -3.2 698,270 + Pf6N2E2_614 0.35 -2.1 698,270 + Pf6N2E2_614 0.35 -1.1 698,271 - Pf6N2E2_614 0.35 -1.2 698,271 - Pf6N2E2_614 0.35 -3.2 698,438 + Pf6N2E2_614 0.49 -0.6 698,438 + Pf6N2E2_614 0.49 -1.5 698,438 + Pf6N2E2_614 0.49 -1.4 698,438 + Pf6N2E2_614 0.49 -0.7 698,439 - Pf6N2E2_614 0.50 -2.4 698,439 - Pf6N2E2_614 0.50 -1.4 698,439 - Pf6N2E2_614 0.50 -1.2 698,439 - Pf6N2E2_614 0.50 +0.2 698,439 - Pf6N2E2_614 0.50 +0.1 698,439 - Pf6N2E2_614 0.50 +0.5 698,472 + Pf6N2E2_614 0.52 -0.0 698,472 + Pf6N2E2_614 0.52 -0.8 698,472 + Pf6N2E2_614 0.52 -2.2 698,472 + Pf6N2E2_614 0.52 -0.8 698,472 + Pf6N2E2_614 0.52 -0.5 698,473 - Pf6N2E2_614 0.52 -0.9 698,473 - Pf6N2E2_614 0.52 -1.4 698,756 + Pf6N2E2_614 0.77 -1.4 698,756 + Pf6N2E2_614 0.77 -1.9 699,067 + +0.3 699,068 - +1.1 699,068 - -0.8 699,098 - +1.0 699,299 + Pf6N2E2_615 0.62 -1.9 699,299 + Pf6N2E2_615 0.62 -0.4 699,300 - Pf6N2E2_615 0.62 -0.8 699,311 + Pf6N2E2_615 0.68 +0.2 699,311 + Pf6N2E2_615 0.68 +0.6 699,312 - Pf6N2E2_615 0.69 +0.6 699,335 + Pf6N2E2_615 0.82 +0.1 699,527 + +0.9 699,527 + -1.4 699,528 - -1.3 699,578 - Pf6N2E2_616 0.29 -0.4 699,812 - -0.1
Or see this region's nucleotide sequence