Experiment: L-Histidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3806 and Pf6N2E2_3807 overlap by 4 nucleotides Pf6N2E2_3807 and Pf6N2E2_3808 are separated by 104 nucleotides Pf6N2E2_3808 and Pf6N2E2_3809 are separated by 121 nucleotides Pf6N2E2_3809 and Pf6N2E2_3810 are separated by 109 nucleotides
Pf6N2E2_3806 - Conserved hypothetical protein (perhaps related to histidine degradation), at 4,435,983 to 4,436,552
_3806
Pf6N2E2_3807 - Histidine utilization repressor, at 4,436,549 to 4,437,298
_3807
Pf6N2E2_3808 - Formiminoglutamic iminohydrolase (EC 3.5.3.13) (from data) , at 4,437,403 to 4,438,767
_3808
Pf6N2E2_3809 - Outer membrane lipoprotein Blc, at 4,438,889 to 4,439,455
_3809
Pf6N2E2_3810 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 4,439,565 to 4,439,819
_3810
Position (kb)
4437
4438
4439 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4436.519 kb on - strand at 4436.555 kb on - strand at 4436.555 kb on - strand at 4436.555 kb on - strand at 4436.555 kb on - strand at 4436.635 kb on + strand, within Pf6N2E2_3807 at 4436.652 kb on - strand, within Pf6N2E2_3807 at 4436.760 kb on + strand, within Pf6N2E2_3807 at 4436.761 kb on - strand, within Pf6N2E2_3807 at 4436.767 kb on - strand, within Pf6N2E2_3807 at 4436.799 kb on + strand, within Pf6N2E2_3807 at 4436.799 kb on + strand, within Pf6N2E2_3807 at 4437.007 kb on + strand, within Pf6N2E2_3807 at 4437.007 kb on + strand, within Pf6N2E2_3807 at 4437.008 kb on - strand, within Pf6N2E2_3807 at 4437.008 kb on - strand, within Pf6N2E2_3807 at 4437.235 kb on - strand at 4437.329 kb on + strand at 4437.330 kb on - strand at 4437.330 kb on - strand at 4437.330 kb on - strand at 4437.330 kb on - strand at 4437.330 kb on - strand at 4437.365 kb on + strand at 4437.365 kb on + strand at 4437.367 kb on + strand at 4437.367 kb on + strand at 4437.368 kb on - strand at 4437.371 kb on + strand at 4437.371 kb on + strand at 4437.371 kb on + strand at 4437.371 kb on + strand at 4437.371 kb on + strand at 4437.372 kb on - strand at 4437.372 kb on - strand at 4437.372 kb on - strand at 4437.374 kb on - strand at 4437.374 kb on - strand at 4437.458 kb on + strand at 4437.458 kb on + strand at 4437.458 kb on + strand at 4437.458 kb on + strand at 4437.458 kb on + strand at 4437.459 kb on - strand at 4437.459 kb on - strand at 4437.658 kb on + strand, within Pf6N2E2_3808 at 4437.659 kb on - strand, within Pf6N2E2_3808 at 4437.715 kb on + strand, within Pf6N2E2_3808 at 4437.716 kb on - strand, within Pf6N2E2_3808 at 4437.716 kb on - strand, within Pf6N2E2_3808 at 4437.739 kb on + strand, within Pf6N2E2_3808 at 4437.763 kb on + strand, within Pf6N2E2_3808 at 4437.763 kb on + strand, within Pf6N2E2_3808 at 4437.763 kb on + strand, within Pf6N2E2_3808 at 4437.763 kb on + strand, within Pf6N2E2_3808 at 4437.764 kb on - strand, within Pf6N2E2_3808 at 4437.764 kb on - strand, within Pf6N2E2_3808 at 4437.764 kb on - strand, within Pf6N2E2_3808 at 4437.794 kb on - strand, within Pf6N2E2_3808 at 4437.877 kb on + strand, within Pf6N2E2_3808 at 4437.877 kb on + strand, within Pf6N2E2_3808 at 4437.878 kb on - strand, within Pf6N2E2_3808 at 4437.878 kb on - strand, within Pf6N2E2_3808 at 4437.949 kb on + strand, within Pf6N2E2_3808 at 4437.950 kb on - strand, within Pf6N2E2_3808 at 4438.169 kb on + strand, within Pf6N2E2_3808 at 4438.169 kb on + strand, within Pf6N2E2_3808 at 4438.450 kb on + strand, within Pf6N2E2_3808 at 4438.467 kb on + strand, within Pf6N2E2_3808 at 4438.591 kb on + strand, within Pf6N2E2_3808 at 4438.591 kb on + strand, within Pf6N2E2_3808 at 4438.592 kb on - strand, within Pf6N2E2_3808 at 4438.592 kb on - strand, within Pf6N2E2_3808 at 4438.592 kb on - strand, within Pf6N2E2_3808 at 4438.659 kb on - strand at 4438.760 kb on + strand at 4438.760 kb on + strand at 4438.761 kb on - strand at 4438.761 kb on - strand at 4438.775 kb on - strand at 4438.826 kb on + strand at 4438.826 kb on + strand at 4438.827 kb on - strand at 4438.827 kb on - strand at 4438.838 kb on + strand at 4438.838 kb on + strand at 4438.839 kb on - strand at 4438.876 kb on + strand at 4438.876 kb on + strand at 4438.876 kb on + strand at 4438.876 kb on + strand at 4438.876 kb on + strand at 4438.877 kb on - strand at 4438.877 kb on - strand at 4438.950 kb on + strand, within Pf6N2E2_3809 at 4438.951 kb on - strand, within Pf6N2E2_3809 at 4438.951 kb on - strand, within Pf6N2E2_3809 at 4439.032 kb on - strand, within Pf6N2E2_3809 at 4439.032 kb on - strand, within Pf6N2E2_3809 at 4439.032 kb on - strand, within Pf6N2E2_3809 at 4439.067 kb on + strand, within Pf6N2E2_3809 at 4439.067 kb on + strand, within Pf6N2E2_3809 at 4439.067 kb on + strand, within Pf6N2E2_3809 at 4439.068 kb on - strand, within Pf6N2E2_3809 at 4439.068 kb on - strand, within Pf6N2E2_3809 at 4439.068 kb on - strand, within Pf6N2E2_3809 at 4439.082 kb on + strand, within Pf6N2E2_3809 at 4439.082 kb on + strand, within Pf6N2E2_3809 at 4439.082 kb on + strand, within Pf6N2E2_3809 at 4439.082 kb on + strand, within Pf6N2E2_3809 at 4439.082 kb on + strand, within Pf6N2E2_3809 at 4439.082 kb on + strand, within Pf6N2E2_3809 at 4439.083 kb on - strand, within Pf6N2E2_3809 at 4439.083 kb on - strand, within Pf6N2E2_3809 at 4439.083 kb on - strand, within Pf6N2E2_3809 at 4439.083 kb on - strand, within Pf6N2E2_3809 at 4439.095 kb on - strand, within Pf6N2E2_3809 at 4439.271 kb on + strand, within Pf6N2E2_3809 at 4439.271 kb on + strand, within Pf6N2E2_3809 at 4439.271 kb on + strand, within Pf6N2E2_3809 at 4439.272 kb on - strand, within Pf6N2E2_3809 at 4439.272 kb on - strand, within Pf6N2E2_3809 at 4439.272 kb on - strand, within Pf6N2E2_3809 at 4439.307 kb on + strand, within Pf6N2E2_3809 at 4439.307 kb on + strand, within Pf6N2E2_3809 at 4439.308 kb on - strand, within Pf6N2E2_3809 at 4439.308 kb on - strand, within Pf6N2E2_3809 at 4439.308 kb on - strand, within Pf6N2E2_3809 at 4439.308 kb on - strand, within Pf6N2E2_3809 at 4439.308 kb on - strand, within Pf6N2E2_3809 at 4439.332 kb on - strand, within Pf6N2E2_3809 at 4439.447 kb on + strand at 4439.459 kb on + strand at 4439.494 kb on + strand at 4439.494 kb on + strand at 4439.495 kb on - strand at 4439.495 kb on - strand at 4439.509 kb on - strand at 4439.525 kb on + strand at 4439.526 kb on - strand at 4439.526 kb on - strand at 4439.526 kb on - strand at 4439.539 kb on + strand at 4439.539 kb on + strand at 4439.539 kb on + strand at 4439.540 kb on - strand at 4439.540 kb on - strand at 4439.540 kb on - strand at 4439.545 kb on + strand at 4439.545 kb on + strand at 4439.546 kb on - strand at 4439.546 kb on - strand at 4439.546 kb on - strand at 4439.566 kb on + strand at 4439.567 kb on - strand at 4439.567 kb on - strand at 4439.611 kb on + strand, within Pf6N2E2_3810 at 4439.743 kb on + strand, within Pf6N2E2_3810 at 4439.743 kb on + strand, within Pf6N2E2_3810 at 4439.744 kb on - strand, within Pf6N2E2_3810
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (N) remove 4,436,519 - +0.4 4,436,555 - -1.9 4,436,555 - +0.4 4,436,555 - -0.1 4,436,555 - +0.3 4,436,635 + Pf6N2E2_3807 0.11 +1.4 4,436,652 - Pf6N2E2_3807 0.14 +0.5 4,436,760 + Pf6N2E2_3807 0.28 -0.2 4,436,761 - Pf6N2E2_3807 0.28 -1.1 4,436,767 - Pf6N2E2_3807 0.29 +1.3 4,436,799 + Pf6N2E2_3807 0.33 -0.1 4,436,799 + Pf6N2E2_3807 0.33 +1.0 4,437,007 + Pf6N2E2_3807 0.61 -1.8 4,437,007 + Pf6N2E2_3807 0.61 +1.6 4,437,008 - Pf6N2E2_3807 0.61 +1.2 4,437,008 - Pf6N2E2_3807 0.61 -0.7 4,437,235 - -2.0 4,437,329 + -0.3 4,437,330 - -0.1 4,437,330 - -2.9 4,437,330 - -0.9 4,437,330 - +1.1 4,437,330 - -2.0 4,437,365 + +0.3 4,437,365 + +1.0 4,437,367 + -0.2 4,437,367 + +0.4 4,437,368 - -0.9 4,437,371 + +0.1 4,437,371 + +0.1 4,437,371 + +0.1 4,437,371 + -0.6 4,437,371 + +0.6 4,437,372 - -2.9 4,437,372 - -3.6 4,437,372 - -2.7 4,437,374 - -2.7 4,437,374 - -3.4 4,437,458 + -3.3 4,437,458 + -4.2 4,437,458 + -2.1 4,437,458 + -1.7 4,437,458 + -2.5 4,437,459 - -2.9 4,437,459 - -3.8 4,437,658 + Pf6N2E2_3808 0.19 -0.9 4,437,659 - Pf6N2E2_3808 0.19 -3.6 4,437,715 + Pf6N2E2_3808 0.23 -2.6 4,437,716 - Pf6N2E2_3808 0.23 -2.9 4,437,716 - Pf6N2E2_3808 0.23 -2.7 4,437,739 + Pf6N2E2_3808 0.25 -2.9 4,437,763 + Pf6N2E2_3808 0.26 -2.9 4,437,763 + Pf6N2E2_3808 0.26 -2.9 4,437,763 + Pf6N2E2_3808 0.26 -2.7 4,437,763 + Pf6N2E2_3808 0.26 -4.5 4,437,764 - Pf6N2E2_3808 0.26 -4.4 4,437,764 - Pf6N2E2_3808 0.26 -2.3 4,437,764 - Pf6N2E2_3808 0.26 -0.9 4,437,794 - Pf6N2E2_3808 0.29 -2.2 4,437,877 + Pf6N2E2_3808 0.35 -1.1 4,437,877 + Pf6N2E2_3808 0.35 -2.3 4,437,878 - Pf6N2E2_3808 0.35 -2.3 4,437,878 - Pf6N2E2_3808 0.35 -2.9 4,437,949 + Pf6N2E2_3808 0.40 -3.0 4,437,950 - Pf6N2E2_3808 0.40 -2.4 4,438,169 + Pf6N2E2_3808 0.56 -2.9 4,438,169 + Pf6N2E2_3808 0.56 -2.1 4,438,450 + Pf6N2E2_3808 0.77 -0.1 4,438,467 + Pf6N2E2_3808 0.78 -3.5 4,438,591 + Pf6N2E2_3808 0.87 -3.3 4,438,591 + Pf6N2E2_3808 0.87 -3.6 4,438,592 - Pf6N2E2_3808 0.87 -3.2 4,438,592 - Pf6N2E2_3808 0.87 -2.2 4,438,592 - Pf6N2E2_3808 0.87 -2.0 4,438,659 - -3.8 4,438,760 + -2.9 4,438,760 + -1.6 4,438,761 - -2.6 4,438,761 - -2.3 4,438,775 - -0.6 4,438,826 + -0.1 4,438,826 + +0.7 4,438,827 - +0.6 4,438,827 - -0.5 4,438,838 + -0.1 4,438,838 + +0.4 4,438,839 - +0.6 4,438,876 + -1.6 4,438,876 + -0.5 4,438,876 + +0.1 4,438,876 + -1.6 4,438,876 + +0.8 4,438,877 - -0.7 4,438,877 - +0.2 4,438,950 + Pf6N2E2_3809 0.11 -0.0 4,438,951 - Pf6N2E2_3809 0.11 +0.8 4,438,951 - Pf6N2E2_3809 0.11 +0.2 4,439,032 - Pf6N2E2_3809 0.25 -0.5 4,439,032 - Pf6N2E2_3809 0.25 +0.3 4,439,032 - Pf6N2E2_3809 0.25 +0.3 4,439,067 + Pf6N2E2_3809 0.31 -0.1 4,439,067 + Pf6N2E2_3809 0.31 +0.6 4,439,067 + Pf6N2E2_3809 0.31 -0.2 4,439,068 - Pf6N2E2_3809 0.32 +1.3 4,439,068 - Pf6N2E2_3809 0.32 -0.2 4,439,068 - Pf6N2E2_3809 0.32 -0.0 4,439,082 + Pf6N2E2_3809 0.34 +0.7 4,439,082 + Pf6N2E2_3809 0.34 +0.0 4,439,082 + Pf6N2E2_3809 0.34 +0.9 4,439,082 + Pf6N2E2_3809 0.34 +0.4 4,439,082 + Pf6N2E2_3809 0.34 -0.0 4,439,082 + Pf6N2E2_3809 0.34 +1.0 4,439,083 - Pf6N2E2_3809 0.34 -0.7 4,439,083 - Pf6N2E2_3809 0.34 -0.2 4,439,083 - Pf6N2E2_3809 0.34 -0.7 4,439,083 - Pf6N2E2_3809 0.34 +0.0 4,439,095 - Pf6N2E2_3809 0.36 -0.4 4,439,271 + Pf6N2E2_3809 0.67 +0.4 4,439,271 + Pf6N2E2_3809 0.67 -0.4 4,439,271 + Pf6N2E2_3809 0.67 +0.1 4,439,272 - Pf6N2E2_3809 0.68 +0.2 4,439,272 - Pf6N2E2_3809 0.68 -0.0 4,439,272 - Pf6N2E2_3809 0.68 +0.1 4,439,307 + Pf6N2E2_3809 0.74 -0.5 4,439,307 + Pf6N2E2_3809 0.74 -0.2 4,439,308 - Pf6N2E2_3809 0.74 +0.4 4,439,308 - Pf6N2E2_3809 0.74 +0.3 4,439,308 - Pf6N2E2_3809 0.74 -0.7 4,439,308 - Pf6N2E2_3809 0.74 -0.4 4,439,308 - Pf6N2E2_3809 0.74 +1.0 4,439,332 - Pf6N2E2_3809 0.78 -0.6 4,439,447 + +0.2 4,439,459 + +1.7 4,439,494 + -0.4 4,439,494 + +0.7 4,439,495 - -0.4 4,439,495 - +0.5 4,439,509 - +1.0 4,439,525 + +0.6 4,439,526 - +0.5 4,439,526 - +0.8 4,439,526 - -1.0 4,439,539 + +0.6 4,439,539 + +1.1 4,439,539 + +1.2 4,439,540 - -0.6 4,439,540 - +1.2 4,439,540 - -1.2 4,439,545 + +0.7 4,439,545 + -0.0 4,439,546 - +1.1 4,439,546 - -0.5 4,439,546 - +0.7 4,439,566 + +0.3 4,439,567 - +0.9 4,439,567 - -0.9 4,439,611 + Pf6N2E2_3810 0.18 +0.3 4,439,743 + Pf6N2E2_3810 0.70 +0.1 4,439,743 + Pf6N2E2_3810 0.70 -0.4 4,439,744 - Pf6N2E2_3810 0.70 -0.6
Or see this region's nucleotide sequence