Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4613 and Pf6N2E2_4614 are separated by 48 nucleotides Pf6N2E2_4614 and Pf6N2E2_4615 are separated by 10 nucleotides Pf6N2E2_4615 and Pf6N2E2_4616 are separated by 158 nucleotides
Pf6N2E2_4613 - twitching motility protein PilG, at 5,338,004 to 5,338,504
_4613
Pf6N2E2_4614 - twitching motility protein PilH, at 5,338,553 to 5,338,918
_4614
Pf6N2E2_4615 - type IV pili signal transduction protein PilI, at 5,338,929 to 5,339,468
_4615
Pf6N2E2_4616 - type IV pilus biogenesis protein PilJ, at 5,339,627 to 5,341,690
_4616
Position (kb)
5338
5339
5340 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 5337.984 kb on + strand at 5338.047 kb on + strand at 5338.595 kb on + strand, within Pf6N2E2_4614 at 5338.595 kb on + strand, within Pf6N2E2_4614 at 5338.595 kb on + strand, within Pf6N2E2_4614 at 5338.596 kb on - strand, within Pf6N2E2_4614 at 5338.853 kb on + strand, within Pf6N2E2_4614 at 5338.854 kb on - strand, within Pf6N2E2_4614 at 5338.854 kb on - strand, within Pf6N2E2_4614 at 5338.854 kb on - strand, within Pf6N2E2_4614 at 5338.854 kb on - strand, within Pf6N2E2_4614 at 5338.854 kb on - strand, within Pf6N2E2_4614 at 5338.854 kb on - strand, within Pf6N2E2_4614 at 5339.067 kb on + strand, within Pf6N2E2_4615 at 5339.068 kb on - strand, within Pf6N2E2_4615 at 5339.528 kb on + strand at 5339.528 kb on + strand at 5339.612 kb on + strand at 5339.613 kb on - strand at 5339.742 kb on - strand at 5339.762 kb on + strand at 5339.763 kb on - strand at 5339.774 kb on + strand at 5339.774 kb on + strand at 5339.774 kb on + strand at 5339.775 kb on - strand at 5339.775 kb on - strand at 5339.775 kb on - strand at 5340.214 kb on + strand, within Pf6N2E2_4616 at 5340.214 kb on + strand, within Pf6N2E2_4616 at 5340.215 kb on - strand, within Pf6N2E2_4616
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 5,337,984 + -0.1 5,338,047 + +0.0 5,338,595 + Pf6N2E2_4614 0.11 +0.7 5,338,595 + Pf6N2E2_4614 0.11 +0.8 5,338,595 + Pf6N2E2_4614 0.11 +0.5 5,338,596 - Pf6N2E2_4614 0.12 +0.1 5,338,853 + Pf6N2E2_4614 0.82 -1.1 5,338,854 - Pf6N2E2_4614 0.82 +0.8 5,338,854 - Pf6N2E2_4614 0.82 +3.8 5,338,854 - Pf6N2E2_4614 0.82 +1.3 5,338,854 - Pf6N2E2_4614 0.82 -0.0 5,338,854 - Pf6N2E2_4614 0.82 +1.0 5,338,854 - Pf6N2E2_4614 0.82 -0.1 5,339,067 + Pf6N2E2_4615 0.26 -0.1 5,339,068 - Pf6N2E2_4615 0.26 -1.3 5,339,528 + +1.5 5,339,528 + -0.4 5,339,612 + -0.5 5,339,613 - +0.7 5,339,742 - +0.3 5,339,762 + +1.5 5,339,763 - +0.4 5,339,774 + +0.2 5,339,774 + -0.1 5,339,774 + +0.1 5,339,775 - +0.2 5,339,775 - +0.2 5,339,775 - +0.4 5,340,214 + Pf6N2E2_4616 0.28 -0.3 5,340,214 + Pf6N2E2_4616 0.28 -0.1 5,340,215 - Pf6N2E2_4616 0.28 -0.1
Or see this region's nucleotide sequence