Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1985 and Pf6N2E2_1986 overlap by 4 nucleotides
Pf6N2E2_1985 - Type IV secretory pathway, VirD4 components, at 2,351,567 to 2,353,117
_1985
Pf6N2E2_1986 - putative membrane protein, at 2,353,114 to 2,353,863
_1986
Position (kb)
2351
2352
2353
2354 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2350.787 kb on + strand at 2350.957 kb on + strand at 2351.019 kb on + strand at 2351.039 kb on + strand at 2351.039 kb on + strand at 2351.039 kb on + strand at 2351.039 kb on + strand at 2351.039 kb on + strand at 2351.039 kb on + strand at 2351.040 kb on - strand at 2351.041 kb on + strand at 2351.073 kb on + strand at 2351.074 kb on - strand at 2351.074 kb on - strand at 2351.074 kb on - strand at 2351.077 kb on + strand at 2351.077 kb on + strand at 2351.078 kb on - strand at 2351.148 kb on + strand at 2351.149 kb on - strand at 2351.221 kb on + strand at 2351.222 kb on - strand at 2351.222 kb on - strand at 2351.222 kb on - strand at 2351.299 kb on + strand at 2351.321 kb on + strand at 2351.347 kb on - strand at 2351.364 kb on - strand at 2351.364 kb on - strand at 2351.364 kb on - strand at 2351.509 kb on - strand at 2351.610 kb on + strand at 2351.694 kb on - strand at 2351.733 kb on - strand, within Pf6N2E2_1985 at 2351.745 kb on + strand, within Pf6N2E2_1985 at 2351.772 kb on - strand, within Pf6N2E2_1985 at 2351.798 kb on - strand, within Pf6N2E2_1985 at 2351.846 kb on - strand, within Pf6N2E2_1985 at 2352.116 kb on + strand, within Pf6N2E2_1985 at 2352.138 kb on - strand, within Pf6N2E2_1985 at 2352.176 kb on + strand, within Pf6N2E2_1985 at 2352.188 kb on + strand, within Pf6N2E2_1985 at 2352.189 kb on - strand, within Pf6N2E2_1985 at 2352.258 kb on + strand, within Pf6N2E2_1985 at 2352.259 kb on - strand, within Pf6N2E2_1985 at 2352.273 kb on - strand, within Pf6N2E2_1985 at 2352.341 kb on + strand, within Pf6N2E2_1985 at 2352.391 kb on + strand, within Pf6N2E2_1985 at 2352.435 kb on - strand, within Pf6N2E2_1985 at 2352.708 kb on + strand, within Pf6N2E2_1985 at 2352.740 kb on + strand, within Pf6N2E2_1985 at 2352.741 kb on - strand, within Pf6N2E2_1985 at 2352.741 kb on - strand, within Pf6N2E2_1985 at 2352.741 kb on - strand, within Pf6N2E2_1985 at 2352.743 kb on - strand, within Pf6N2E2_1985 at 2352.992 kb on + strand at 2352.992 kb on + strand at 2353.001 kb on - strand at 2353.008 kb on - strand at 2353.049 kb on + strand at 2353.050 kb on - strand at 2353.069 kb on + strand at 2353.182 kb on + strand at 2353.254 kb on + strand, within Pf6N2E2_1986 at 2353.254 kb on + strand, within Pf6N2E2_1986 at 2353.255 kb on - strand, within Pf6N2E2_1986 at 2353.258 kb on + strand, within Pf6N2E2_1986 at 2353.258 kb on + strand, within Pf6N2E2_1986 at 2353.258 kb on + strand, within Pf6N2E2_1986 at 2353.258 kb on + strand, within Pf6N2E2_1986 at 2353.259 kb on - strand, within Pf6N2E2_1986 at 2353.259 kb on - strand at 2353.400 kb on - strand, within Pf6N2E2_1986 at 2353.498 kb on + strand, within Pf6N2E2_1986 at 2353.501 kb on + strand, within Pf6N2E2_1986 at 2353.502 kb on - strand, within Pf6N2E2_1986 at 2353.517 kb on - strand, within Pf6N2E2_1986 at 2353.517 kb on - strand, within Pf6N2E2_1986 at 2353.517 kb on - strand, within Pf6N2E2_1986 at 2353.518 kb on + strand, within Pf6N2E2_1986 at 2353.518 kb on + strand, within Pf6N2E2_1986 at 2353.518 kb on + strand, within Pf6N2E2_1986 at 2353.519 kb on - strand, within Pf6N2E2_1986 at 2353.615 kb on - strand, within Pf6N2E2_1986 at 2353.649 kb on + strand, within Pf6N2E2_1986 at 2353.693 kb on + strand, within Pf6N2E2_1986 at 2353.797 kb on - strand at 2353.856 kb on - strand at 2353.879 kb on + strand at 2353.959 kb on + strand at 2354.041 kb on + strand at 2354.084 kb on + strand at 2354.085 kb on - strand at 2354.092 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 2,350,787 + -0.2 2,350,957 + +0.1 2,351,019 + +0.8 2,351,039 + -0.7 2,351,039 + +1.0 2,351,039 + +3.2 2,351,039 + -0.7 2,351,039 + +0.2 2,351,039 + +0.2 2,351,040 - +0.0 2,351,041 + +0.4 2,351,073 + -0.3 2,351,074 - +0.5 2,351,074 - +0.4 2,351,074 - +0.7 2,351,077 + +1.8 2,351,077 + +0.0 2,351,078 - -0.6 2,351,148 + +0.7 2,351,149 - +0.4 2,351,221 + +0.2 2,351,222 - -0.8 2,351,222 - +0.4 2,351,222 - -1.3 2,351,299 + +0.7 2,351,321 + +0.9 2,351,347 - -0.0 2,351,364 - -0.7 2,351,364 - -0.6 2,351,364 - +0.2 2,351,509 - -0.0 2,351,610 + +1.0 2,351,694 - +0.8 2,351,733 - Pf6N2E2_1985 0.11 -1.0 2,351,745 + Pf6N2E2_1985 0.11 -1.0 2,351,772 - Pf6N2E2_1985 0.13 +2.0 2,351,798 - Pf6N2E2_1985 0.15 -1.5 2,351,846 - Pf6N2E2_1985 0.18 -1.4 2,352,116 + Pf6N2E2_1985 0.35 -0.1 2,352,138 - Pf6N2E2_1985 0.37 -1.0 2,352,176 + Pf6N2E2_1985 0.39 +0.2 2,352,188 + Pf6N2E2_1985 0.40 -0.3 2,352,189 - Pf6N2E2_1985 0.40 -0.7 2,352,258 + Pf6N2E2_1985 0.45 -0.8 2,352,259 - Pf6N2E2_1985 0.45 +0.2 2,352,273 - Pf6N2E2_1985 0.46 +0.2 2,352,341 + Pf6N2E2_1985 0.50 -0.3 2,352,391 + Pf6N2E2_1985 0.53 +0.1 2,352,435 - Pf6N2E2_1985 0.56 -1.2 2,352,708 + Pf6N2E2_1985 0.74 -0.5 2,352,740 + Pf6N2E2_1985 0.76 -1.4 2,352,741 - Pf6N2E2_1985 0.76 +0.3 2,352,741 - Pf6N2E2_1985 0.76 -0.0 2,352,741 - Pf6N2E2_1985 0.76 -0.7 2,352,743 - Pf6N2E2_1985 0.76 +0.3 2,352,992 + -1.2 2,352,992 + +0.7 2,353,001 - +0.5 2,353,008 - +0.7 2,353,049 + +0.4 2,353,050 - +0.6 2,353,069 + +0.1 2,353,182 + -0.3 2,353,254 + Pf6N2E2_1986 0.19 +0.5 2,353,254 + Pf6N2E2_1986 0.19 +0.8 2,353,255 - Pf6N2E2_1986 0.19 +0.3 2,353,258 + Pf6N2E2_1986 0.19 +1.0 2,353,258 + Pf6N2E2_1986 0.19 -1.4 2,353,258 + Pf6N2E2_1986 0.19 -1.3 2,353,258 + Pf6N2E2_1986 0.19 +0.5 2,353,259 - Pf6N2E2_1986 0.19 -1.0 2,353,259 - +1.6 2,353,400 - Pf6N2E2_1986 0.38 -1.1 2,353,498 + Pf6N2E2_1986 0.51 -0.8 2,353,501 + Pf6N2E2_1986 0.52 -0.7 2,353,502 - Pf6N2E2_1986 0.52 -0.0 2,353,517 - Pf6N2E2_1986 0.54 +0.7 2,353,517 - Pf6N2E2_1986 0.54 -0.0 2,353,517 - Pf6N2E2_1986 0.54 +1.1 2,353,518 + Pf6N2E2_1986 0.54 +0.5 2,353,518 + Pf6N2E2_1986 0.54 +0.2 2,353,518 + Pf6N2E2_1986 0.54 +1.0 2,353,519 - Pf6N2E2_1986 0.54 +0.8 2,353,615 - Pf6N2E2_1986 0.67 +0.2 2,353,649 + Pf6N2E2_1986 0.71 -0.5 2,353,693 + Pf6N2E2_1986 0.77 +0.4 2,353,797 - +1.3 2,353,856 - +0.7 2,353,879 + -0.5 2,353,959 + -1.0 2,354,041 + +0.1 2,354,084 + +0.3 2,354,085 - +0.6 2,354,092 + +1.0
Or see this region's nucleotide sequence