Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1705 and Pf6N2E2_1706 overlap by 4 nucleotides Pf6N2E2_1706 and Pf6N2E2_1707 are separated by 4 nucleotides Pf6N2E2_1707 and Pf6N2E2_1708 overlap by 42 nucleotides Pf6N2E2_1708 and Pf6N2E2_1709 are separated by 291 nucleotides
Pf6N2E2_1705 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 2,013,787 to 2,014,557
_1705
Pf6N2E2_1706 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 2,014,554 to 2,015,525
_1706
Pf6N2E2_1707 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases, at 2,015,530 to 2,016,915
_1707
Pf6N2E2_1708 - hypothetical protein, at 2,016,874 to 2,017,026
_1708
Pf6N2E2_1709 - multidrug resistance transporter, Bcr/CflA family, at 2,017,318 to 2,018,538
_1709
Position (kb)
2015
2016
2017 Strain fitness (log2 ratio)
-2
-1
0
1 at 2014.573 kb on + strand at 2014.574 kb on - strand at 2014.574 kb on - strand at 2014.611 kb on + strand at 2015.187 kb on + strand, within Pf6N2E2_1706 at 2015.199 kb on + strand, within Pf6N2E2_1706 at 2015.199 kb on + strand, within Pf6N2E2_1706 at 2015.199 kb on + strand, within Pf6N2E2_1706 at 2015.200 kb on - strand, within Pf6N2E2_1706 at 2015.515 kb on + strand at 2015.515 kb on + strand at 2015.516 kb on - strand at 2015.757 kb on - strand, within Pf6N2E2_1707 at 2016.036 kb on - strand, within Pf6N2E2_1707 at 2016.044 kb on + strand, within Pf6N2E2_1707 at 2016.044 kb on + strand, within Pf6N2E2_1707 at 2016.443 kb on + strand, within Pf6N2E2_1707 at 2016.444 kb on - strand, within Pf6N2E2_1707 at 2016.459 kb on - strand, within Pf6N2E2_1707 at 2016.536 kb on + strand, within Pf6N2E2_1707 at 2016.536 kb on + strand, within Pf6N2E2_1707 at 2016.536 kb on + strand, within Pf6N2E2_1707 at 2016.762 kb on - strand, within Pf6N2E2_1707 at 2016.762 kb on - strand, within Pf6N2E2_1707 at 2016.762 kb on - strand, within Pf6N2E2_1707 at 2016.762 kb on - strand, within Pf6N2E2_1707 at 2016.762 kb on - strand, within Pf6N2E2_1707 at 2016.762 kb on - strand, within Pf6N2E2_1707 at 2016.762 kb on - strand, within Pf6N2E2_1707 at 2016.766 kb on - strand, within Pf6N2E2_1707 at 2016.852 kb on - strand at 2017.127 kb on + strand at 2017.128 kb on - strand at 2017.128 kb on - strand at 2017.128 kb on - strand at 2017.128 kb on - strand at 2017.138 kb on + strand at 2017.139 kb on - strand at 2017.139 kb on - strand at 2017.139 kb on - strand at 2017.195 kb on + strand at 2017.300 kb on + strand at 2017.301 kb on - strand at 2017.317 kb on + strand at 2017.317 kb on + strand at 2017.317 kb on + strand at 2017.317 kb on + strand at 2017.317 kb on + strand at 2017.317 kb on + strand at 2017.317 kb on + strand at 2017.317 kb on + strand at 2017.318 kb on - strand at 2017.318 kb on - strand at 2017.318 kb on - strand at 2017.318 kb on - strand at 2017.904 kb on + strand, within Pf6N2E2_1709 at 2017.904 kb on + strand, within Pf6N2E2_1709
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 2,014,573 + +0.1 2,014,574 - -0.0 2,014,574 - -1.3 2,014,611 + -0.5 2,015,187 + Pf6N2E2_1706 0.65 +0.4 2,015,199 + Pf6N2E2_1706 0.66 -0.2 2,015,199 + Pf6N2E2_1706 0.66 +1.0 2,015,199 + Pf6N2E2_1706 0.66 -0.3 2,015,200 - Pf6N2E2_1706 0.66 -0.2 2,015,515 + -0.7 2,015,515 + -0.8 2,015,516 - -0.1 2,015,757 - Pf6N2E2_1707 0.16 -1.1 2,016,036 - Pf6N2E2_1707 0.37 -0.0 2,016,044 + Pf6N2E2_1707 0.37 -0.0 2,016,044 + Pf6N2E2_1707 0.37 +0.3 2,016,443 + Pf6N2E2_1707 0.66 -0.5 2,016,444 - Pf6N2E2_1707 0.66 -0.8 2,016,459 - Pf6N2E2_1707 0.67 -1.2 2,016,536 + Pf6N2E2_1707 0.73 -1.2 2,016,536 + Pf6N2E2_1707 0.73 +0.1 2,016,536 + Pf6N2E2_1707 0.73 -0.9 2,016,762 - Pf6N2E2_1707 0.89 -0.1 2,016,762 - Pf6N2E2_1707 0.89 -0.3 2,016,762 - Pf6N2E2_1707 0.89 -0.3 2,016,762 - Pf6N2E2_1707 0.89 -0.8 2,016,762 - Pf6N2E2_1707 0.89 -0.0 2,016,762 - Pf6N2E2_1707 0.89 -0.2 2,016,762 - Pf6N2E2_1707 0.89 -0.6 2,016,766 - Pf6N2E2_1707 0.89 -0.0 2,016,852 - -1.0 2,017,127 + -0.3 2,017,128 - +0.1 2,017,128 - -0.1 2,017,128 - +0.6 2,017,128 - -0.3 2,017,138 + -0.5 2,017,139 - +0.5 2,017,139 - +0.4 2,017,139 - -0.2 2,017,195 + -0.3 2,017,300 + +1.6 2,017,301 - -0.1 2,017,317 + +0.4 2,017,317 + -2.3 2,017,317 + -0.5 2,017,317 + -0.2 2,017,317 + +0.5 2,017,317 + -0.1 2,017,317 + +0.3 2,017,317 + +1.5 2,017,318 - -0.5 2,017,318 - +0.2 2,017,318 - -0.3 2,017,318 - +1.1 2,017,904 + Pf6N2E2_1709 0.48 +1.6 2,017,904 + Pf6N2E2_1709 0.48 +0.2
Or see this region's nucleotide sequence