Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_381 and Pf6N2E2_382 are separated by 249 nucleotides
Pf6N2E2_381 - hypothetical protein, at 447,554 to 448,942
_381
Pf6N2E2_382 - RNA:NAD 2'-phosphotransferase, at 449,192 to 449,776
_382
Position (kb)
449
450 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 448.201 kb on + strand, within Pf6N2E2_381 at 448.201 kb on + strand, within Pf6N2E2_381 at 448.202 kb on - strand, within Pf6N2E2_381 at 448.356 kb on + strand, within Pf6N2E2_381 at 448.356 kb on + strand, within Pf6N2E2_381 at 448.357 kb on - strand, within Pf6N2E2_381 at 448.357 kb on - strand, within Pf6N2E2_381 at 448.360 kb on - strand, within Pf6N2E2_381 at 448.404 kb on + strand, within Pf6N2E2_381 at 448.404 kb on + strand, within Pf6N2E2_381 at 448.404 kb on + strand, within Pf6N2E2_381 at 448.405 kb on - strand, within Pf6N2E2_381 at 448.470 kb on + strand, within Pf6N2E2_381 at 448.588 kb on + strand, within Pf6N2E2_381 at 448.589 kb on - strand, within Pf6N2E2_381 at 448.683 kb on + strand, within Pf6N2E2_381 at 448.708 kb on - strand, within Pf6N2E2_381 at 448.708 kb on - strand, within Pf6N2E2_381 at 448.769 kb on + strand, within Pf6N2E2_381 at 448.769 kb on + strand, within Pf6N2E2_381 at 448.769 kb on + strand, within Pf6N2E2_381 at 448.769 kb on + strand, within Pf6N2E2_381 at 448.769 kb on + strand, within Pf6N2E2_381 at 448.770 kb on - strand, within Pf6N2E2_381 at 448.770 kb on - strand, within Pf6N2E2_381 at 448.956 kb on - strand at 449.004 kb on + strand at 449.004 kb on + strand at 449.009 kb on + strand at 449.009 kb on + strand at 449.176 kb on + strand at 449.177 kb on - strand at 449.177 kb on - strand at 449.177 kb on - strand at 449.177 kb on - strand at 449.177 kb on - strand at 449.178 kb on + strand at 449.197 kb on + strand at 449.197 kb on + strand at 449.198 kb on - strand at 449.206 kb on + strand at 449.206 kb on + strand at 449.207 kb on - strand at 449.214 kb on - strand at 449.226 kb on + strand at 449.226 kb on + strand at 449.227 kb on - strand at 449.227 kb on - strand at 449.306 kb on + strand, within Pf6N2E2_382 at 449.307 kb on - strand, within Pf6N2E2_382 at 449.307 kb on - strand, within Pf6N2E2_382 at 449.337 kb on + strand, within Pf6N2E2_382 at 449.457 kb on + strand, within Pf6N2E2_382 at 449.457 kb on + strand, within Pf6N2E2_382 at 449.457 kb on + strand, within Pf6N2E2_382 at 449.457 kb on + strand, within Pf6N2E2_382 at 449.458 kb on - strand, within Pf6N2E2_382 at 449.458 kb on - strand, within Pf6N2E2_382 at 449.458 kb on - strand, within Pf6N2E2_382 at 449.458 kb on - strand, within Pf6N2E2_382 at 449.604 kb on + strand, within Pf6N2E2_382 at 449.614 kb on + strand, within Pf6N2E2_382 at 449.615 kb on - strand, within Pf6N2E2_382 at 449.647 kb on + strand, within Pf6N2E2_382 at 449.648 kb on - strand, within Pf6N2E2_382 at 449.682 kb on - strand, within Pf6N2E2_382 at 449.682 kb on - strand, within Pf6N2E2_382 at 449.708 kb on + strand, within Pf6N2E2_382 at 449.709 kb on - strand, within Pf6N2E2_382 at 449.711 kb on + strand, within Pf6N2E2_382 at 449.711 kb on + strand at 449.711 kb on + strand, within Pf6N2E2_382 at 449.711 kb on + strand, within Pf6N2E2_382 at 449.711 kb on + strand, within Pf6N2E2_382 at 449.712 kb on - strand, within Pf6N2E2_382 at 449.712 kb on - strand, within Pf6N2E2_382 at 449.715 kb on + strand, within Pf6N2E2_382 at 449.715 kb on + strand, within Pf6N2E2_382 at 449.715 kb on + strand, within Pf6N2E2_382 at 449.716 kb on - strand, within Pf6N2E2_382 at 449.791 kb on + strand at 449.791 kb on + strand at 449.792 kb on - strand at 449.792 kb on - strand at 449.792 kb on - strand at 449.794 kb on + strand at 449.794 kb on + strand at 449.795 kb on - strand at 449.795 kb on - strand at 449.795 kb on - strand at 449.809 kb on + strand at 449.809 kb on + strand at 449.810 kb on - strand at 449.810 kb on - strand at 449.810 kb on - strand at 449.879 kb on + strand at 450.008 kb on - strand at 450.008 kb on - strand at 450.008 kb on - strand at 450.008 kb on - strand at 450.010 kb on + strand at 450.011 kb on - strand at 450.011 kb on - strand at 450.052 kb on + strand at 450.053 kb on - strand at 450.053 kb on - strand at 450.222 kb on + strand at 450.270 kb on + strand at 450.270 kb on + strand at 450.270 kb on + strand at 450.270 kb on + strand at 450.270 kb on + strand at 450.271 kb on - strand at 450.271 kb on - strand at 450.271 kb on - strand at 450.271 kb on - strand at 450.307 kb on + strand at 450.307 kb on + strand at 450.308 kb on - strand at 450.370 kb on + strand at 450.402 kb on + strand at 450.402 kb on + strand at 450.402 kb on + strand at 450.402 kb on + strand at 450.403 kb on - strand at 450.403 kb on - strand at 450.403 kb on - strand at 450.403 kb on - strand at 450.403 kb on - strand at 450.403 kb on - strand at 450.403 kb on - strand at 450.403 kb on - strand at 450.405 kb on - strand at 450.405 kb on - strand at 450.405 kb on - strand at 450.405 kb on - strand at 450.438 kb on + strand at 450.438 kb on + strand at 450.438 kb on + strand at 450.439 kb on - strand at 450.757 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 448,201 + Pf6N2E2_381 0.47 +0.3 448,201 + Pf6N2E2_381 0.47 -1.3 448,202 - Pf6N2E2_381 0.47 +0.8 448,356 + Pf6N2E2_381 0.58 -0.4 448,356 + Pf6N2E2_381 0.58 -0.5 448,357 - Pf6N2E2_381 0.58 +2.3 448,357 - Pf6N2E2_381 0.58 +0.8 448,360 - Pf6N2E2_381 0.58 -1.9 448,404 + Pf6N2E2_381 0.61 -1.6 448,404 + Pf6N2E2_381 0.61 +0.3 448,404 + Pf6N2E2_381 0.61 -1.4 448,405 - Pf6N2E2_381 0.61 +0.3 448,470 + Pf6N2E2_381 0.66 +0.7 448,588 + Pf6N2E2_381 0.74 -0.9 448,589 - Pf6N2E2_381 0.75 -0.0 448,683 + Pf6N2E2_381 0.81 +0.2 448,708 - Pf6N2E2_381 0.83 -0.5 448,708 - Pf6N2E2_381 0.83 +0.3 448,769 + Pf6N2E2_381 0.87 +0.3 448,769 + Pf6N2E2_381 0.87 -0.4 448,769 + Pf6N2E2_381 0.87 -0.4 448,769 + Pf6N2E2_381 0.87 +0.3 448,769 + Pf6N2E2_381 0.87 +1.8 448,770 - Pf6N2E2_381 0.88 -0.5 448,770 - Pf6N2E2_381 0.88 +0.3 448,956 - +0.5 449,004 + +0.9 449,004 + +0.4 449,009 + +0.3 449,009 + -0.1 449,176 + -0.3 449,177 - +0.1 449,177 - +0.1 449,177 - -0.1 449,177 - -0.5 449,177 - +0.6 449,178 + +1.0 449,197 + -0.2 449,197 + +0.3 449,198 - +0.9 449,206 + +0.9 449,206 + -0.5 449,207 - -0.3 449,214 - +0.2 449,226 + +0.5 449,226 + -0.0 449,227 - +0.4 449,227 - -0.1 449,306 + Pf6N2E2_382 0.19 -0.1 449,307 - Pf6N2E2_382 0.20 -0.4 449,307 - Pf6N2E2_382 0.20 +0.6 449,337 + Pf6N2E2_382 0.25 +1.0 449,457 + Pf6N2E2_382 0.45 -0.3 449,457 + Pf6N2E2_382 0.45 +2.2 449,457 + Pf6N2E2_382 0.45 -1.1 449,457 + Pf6N2E2_382 0.45 -0.0 449,458 - Pf6N2E2_382 0.45 +0.5 449,458 - Pf6N2E2_382 0.45 +0.5 449,458 - Pf6N2E2_382 0.45 +0.2 449,458 - Pf6N2E2_382 0.45 +0.6 449,604 + Pf6N2E2_382 0.70 +0.8 449,614 + Pf6N2E2_382 0.72 -0.0 449,615 - Pf6N2E2_382 0.72 +1.5 449,647 + Pf6N2E2_382 0.78 +1.3 449,648 - Pf6N2E2_382 0.78 +1.8 449,682 - Pf6N2E2_382 0.84 -0.5 449,682 - Pf6N2E2_382 0.84 +0.5 449,708 + Pf6N2E2_382 0.88 +0.7 449,709 - Pf6N2E2_382 0.88 -2.0 449,711 + Pf6N2E2_382 0.89 -1.8 449,711 + +1.3 449,711 + Pf6N2E2_382 0.89 -0.6 449,711 + Pf6N2E2_382 0.89 +0.1 449,711 + Pf6N2E2_382 0.89 +0.8 449,712 - Pf6N2E2_382 0.89 +0.7 449,712 - Pf6N2E2_382 0.89 +0.1 449,715 + Pf6N2E2_382 0.89 -0.3 449,715 + Pf6N2E2_382 0.89 +0.5 449,715 + Pf6N2E2_382 0.89 +0.0 449,716 - Pf6N2E2_382 0.90 +1.4 449,791 + -0.6 449,791 + +0.5 449,792 - +0.2 449,792 - +0.5 449,792 - +0.6 449,794 + +0.2 449,794 + +3.3 449,795 - -0.3 449,795 - -1.0 449,795 - +0.1 449,809 + -1.3 449,809 + +0.7 449,810 - -0.7 449,810 - -0.2 449,810 - -0.7 449,879 + -0.7 450,008 - +1.3 450,008 - +0.8 450,008 - +0.3 450,008 - +0.4 450,010 + -0.1 450,011 - +0.1 450,011 - -0.4 450,052 + +0.5 450,053 - +0.5 450,053 - -0.1 450,222 + -0.5 450,270 + +0.4 450,270 + +1.6 450,270 + +0.4 450,270 + -0.8 450,270 + +1.0 450,271 - -1.0 450,271 - -0.2 450,271 - +0.7 450,271 - +0.6 450,307 + -0.3 450,307 + -0.1 450,308 - -0.1 450,370 + +0.7 450,402 + -0.9 450,402 + +0.6 450,402 + +0.7 450,402 + -1.3 450,403 - +0.6 450,403 - -0.6 450,403 - -0.5 450,403 - +0.3 450,403 - -0.5 450,403 - +0.1 450,403 - -0.9 450,403 - +0.4 450,405 - -0.5 450,405 - +0.8 450,405 - +0.9 450,405 - +1.4 450,438 + -1.0 450,438 + +0.9 450,438 + +0.1 450,439 - +0.5 450,757 + +0.5
Or see this region's nucleotide sequence