Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1477 and Pf6N2E2_1478 are separated by 37 nucleotides Pf6N2E2_1478 and Pf6N2E2_1479 are separated by 160 nucleotides Pf6N2E2_1479 and Pf6N2E2_1480 are separated by 20 nucleotides
Pf6N2E2_1477 - hypothetical protein, at 1,726,379 to 1,726,672
_1477
Pf6N2E2_1478 - hypothetical protein, at 1,726,710 to 1,727,276
_1478
Pf6N2E2_1479 - Subtilisin-like serine proteases, at 1,727,437 to 1,729,140
_1479
Pf6N2E2_1480 - SAM-dependent methyltransferases, at 1,729,161 to 1,729,868
_1480
Position (kb)
1727
1728
1729
1730 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1726.450 kb on + strand, within Pf6N2E2_1477 at 1726.450 kb on + strand, within Pf6N2E2_1477 at 1726.450 kb on + strand, within Pf6N2E2_1477 at 1726.450 kb on + strand, within Pf6N2E2_1477 at 1726.450 kb on + strand, within Pf6N2E2_1477 at 1726.450 kb on + strand, within Pf6N2E2_1477 at 1726.451 kb on - strand, within Pf6N2E2_1477 at 1726.451 kb on - strand, within Pf6N2E2_1477 at 1726.451 kb on - strand, within Pf6N2E2_1477 at 1726.607 kb on + strand, within Pf6N2E2_1477 at 1726.607 kb on + strand, within Pf6N2E2_1477 at 1726.607 kb on + strand, within Pf6N2E2_1477 at 1726.746 kb on - strand at 1726.843 kb on + strand, within Pf6N2E2_1478 at 1726.843 kb on + strand, within Pf6N2E2_1478 at 1726.843 kb on + strand, within Pf6N2E2_1478 at 1726.903 kb on - strand, within Pf6N2E2_1478 at 1726.903 kb on - strand, within Pf6N2E2_1478 at 1726.903 kb on - strand, within Pf6N2E2_1478 at 1727.026 kb on - strand, within Pf6N2E2_1478 at 1727.026 kb on - strand, within Pf6N2E2_1478 at 1727.038 kb on - strand, within Pf6N2E2_1478 at 1727.070 kb on + strand, within Pf6N2E2_1478 at 1727.070 kb on + strand, within Pf6N2E2_1478 at 1727.070 kb on + strand, within Pf6N2E2_1478 at 1727.070 kb on + strand, within Pf6N2E2_1478 at 1727.070 kb on + strand, within Pf6N2E2_1478 at 1727.070 kb on + strand, within Pf6N2E2_1478 at 1727.071 kb on - strand, within Pf6N2E2_1478 at 1727.071 kb on - strand, within Pf6N2E2_1478 at 1727.184 kb on + strand, within Pf6N2E2_1478 at 1727.184 kb on + strand, within Pf6N2E2_1478 at 1727.184 kb on + strand, within Pf6N2E2_1478 at 1727.184 kb on + strand, within Pf6N2E2_1478 at 1727.185 kb on - strand, within Pf6N2E2_1478 at 1727.185 kb on - strand, within Pf6N2E2_1478 at 1727.185 kb on - strand, within Pf6N2E2_1478 at 1727.185 kb on - strand, within Pf6N2E2_1478 at 1727.258 kb on - strand at 1727.268 kb on - strand at 1727.331 kb on + strand at 1727.331 kb on + strand at 1727.331 kb on + strand at 1727.332 kb on - strand at 1727.332 kb on - strand at 1727.382 kb on - strand at 1727.400 kb on + strand at 1727.711 kb on + strand, within Pf6N2E2_1479 at 1727.711 kb on + strand, within Pf6N2E2_1479 at 1727.712 kb on - strand, within Pf6N2E2_1479 at 1727.712 kb on - strand, within Pf6N2E2_1479 at 1727.735 kb on + strand, within Pf6N2E2_1479 at 1727.764 kb on + strand at 1727.764 kb on + strand, within Pf6N2E2_1479 at 1727.765 kb on - strand, within Pf6N2E2_1479 at 1727.840 kb on - strand, within Pf6N2E2_1479 at 1727.840 kb on - strand, within Pf6N2E2_1479 at 1727.881 kb on + strand, within Pf6N2E2_1479 at 1728.718 kb on - strand, within Pf6N2E2_1479 at 1728.745 kb on + strand, within Pf6N2E2_1479 at 1728.746 kb on - strand, within Pf6N2E2_1479 at 1728.746 kb on - strand, within Pf6N2E2_1479 at 1728.951 kb on - strand, within Pf6N2E2_1479 at 1728.951 kb on - strand, within Pf6N2E2_1479 at 1729.146 kb on + strand at 1729.146 kb on + strand at 1729.147 kb on - strand at 1729.147 kb on - strand at 1729.147 kb on - strand at 1729.157 kb on + strand at 1729.157 kb on + strand at 1729.157 kb on + strand at 1729.385 kb on - strand, within Pf6N2E2_1480 at 1729.428 kb on + strand, within Pf6N2E2_1480 at 1729.549 kb on - strand, within Pf6N2E2_1480 at 1729.602 kb on + strand, within Pf6N2E2_1480 at 1729.602 kb on + strand, within Pf6N2E2_1480 at 1729.602 kb on + strand, within Pf6N2E2_1480 at 1729.756 kb on - strand, within Pf6N2E2_1480 at 1729.775 kb on - strand, within Pf6N2E2_1480 at 1729.775 kb on - strand, within Pf6N2E2_1480 at 1729.775 kb on - strand, within Pf6N2E2_1480 at 1729.790 kb on + strand, within Pf6N2E2_1480 at 1729.791 kb on - strand, within Pf6N2E2_1480 at 1729.903 kb on - strand at 1729.903 kb on - strand at 1729.955 kb on - strand at 1729.955 kb on - strand at 1729.955 kb on - strand at 1729.955 kb on - strand at 1729.981 kb on + strand at 1730.135 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 1,726,450 + Pf6N2E2_1477 0.24 +0.2 1,726,450 + Pf6N2E2_1477 0.24 +0.5 1,726,450 + Pf6N2E2_1477 0.24 -1.1 1,726,450 + Pf6N2E2_1477 0.24 -0.8 1,726,450 + Pf6N2E2_1477 0.24 -0.2 1,726,450 + Pf6N2E2_1477 0.24 +1.0 1,726,451 - Pf6N2E2_1477 0.24 +0.6 1,726,451 - Pf6N2E2_1477 0.24 -2.7 1,726,451 - Pf6N2E2_1477 0.24 +0.4 1,726,607 + Pf6N2E2_1477 0.78 -1.3 1,726,607 + Pf6N2E2_1477 0.78 -0.1 1,726,607 + Pf6N2E2_1477 0.78 -0.2 1,726,746 - +1.0 1,726,843 + Pf6N2E2_1478 0.23 -0.9 1,726,843 + Pf6N2E2_1478 0.23 -0.1 1,726,843 + Pf6N2E2_1478 0.23 -1.3 1,726,903 - Pf6N2E2_1478 0.34 +0.4 1,726,903 - Pf6N2E2_1478 0.34 +0.4 1,726,903 - Pf6N2E2_1478 0.34 +2.4 1,727,026 - Pf6N2E2_1478 0.56 +0.3 1,727,026 - Pf6N2E2_1478 0.56 +0.2 1,727,038 - Pf6N2E2_1478 0.58 +0.2 1,727,070 + Pf6N2E2_1478 0.63 -3.4 1,727,070 + Pf6N2E2_1478 0.63 -0.3 1,727,070 + Pf6N2E2_1478 0.63 +0.5 1,727,070 + Pf6N2E2_1478 0.63 -0.1 1,727,070 + Pf6N2E2_1478 0.63 +0.2 1,727,070 + Pf6N2E2_1478 0.63 -1.0 1,727,071 - Pf6N2E2_1478 0.64 -0.5 1,727,071 - Pf6N2E2_1478 0.64 -0.3 1,727,184 + Pf6N2E2_1478 0.84 +0.8 1,727,184 + Pf6N2E2_1478 0.84 +0.7 1,727,184 + Pf6N2E2_1478 0.84 +0.9 1,727,184 + Pf6N2E2_1478 0.84 -0.0 1,727,185 - Pf6N2E2_1478 0.84 -0.7 1,727,185 - Pf6N2E2_1478 0.84 +0.5 1,727,185 - Pf6N2E2_1478 0.84 -2.1 1,727,185 - Pf6N2E2_1478 0.84 +1.9 1,727,258 - -0.9 1,727,268 - +0.3 1,727,331 + +0.3 1,727,331 + +0.1 1,727,331 + +0.6 1,727,332 - +0.4 1,727,332 - -0.5 1,727,382 - -0.5 1,727,400 + -2.0 1,727,711 + Pf6N2E2_1479 0.16 +0.2 1,727,711 + Pf6N2E2_1479 0.16 +0.9 1,727,712 - Pf6N2E2_1479 0.16 +0.4 1,727,712 - Pf6N2E2_1479 0.16 -0.1 1,727,735 + Pf6N2E2_1479 0.17 +2.0 1,727,764 + +2.6 1,727,764 + Pf6N2E2_1479 0.19 -0.2 1,727,765 - Pf6N2E2_1479 0.19 +1.0 1,727,840 - Pf6N2E2_1479 0.24 -0.0 1,727,840 - Pf6N2E2_1479 0.24 +0.4 1,727,881 + Pf6N2E2_1479 0.26 +0.1 1,728,718 - Pf6N2E2_1479 0.75 +2.0 1,728,745 + Pf6N2E2_1479 0.77 +0.7 1,728,746 - Pf6N2E2_1479 0.77 -2.1 1,728,746 - Pf6N2E2_1479 0.77 -0.2 1,728,951 - Pf6N2E2_1479 0.89 +0.8 1,728,951 - Pf6N2E2_1479 0.89 +0.3 1,729,146 + +0.5 1,729,146 + +1.2 1,729,147 - +1.1 1,729,147 - +0.3 1,729,147 - -0.2 1,729,157 + +0.6 1,729,157 + +0.6 1,729,157 + +0.3 1,729,385 - Pf6N2E2_1480 0.32 -0.3 1,729,428 + Pf6N2E2_1480 0.38 +0.9 1,729,549 - Pf6N2E2_1480 0.55 -0.2 1,729,602 + Pf6N2E2_1480 0.62 +0.9 1,729,602 + Pf6N2E2_1480 0.62 +1.5 1,729,602 + Pf6N2E2_1480 0.62 +0.2 1,729,756 - Pf6N2E2_1480 0.84 +0.0 1,729,775 - Pf6N2E2_1480 0.87 -0.0 1,729,775 - Pf6N2E2_1480 0.87 -0.1 1,729,775 - Pf6N2E2_1480 0.87 +0.1 1,729,790 + Pf6N2E2_1480 0.89 +0.3 1,729,791 - Pf6N2E2_1480 0.89 -0.5 1,729,903 - +0.6 1,729,903 - -0.6 1,729,955 - -0.5 1,729,955 - -0.1 1,729,955 - +1.0 1,729,955 - -0.3 1,729,981 + -0.5 1,730,135 - -0.7
Or see this region's nucleotide sequence