Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_102 and Pf6N2E2_103 are separated by 355 nucleotides Pf6N2E2_103 and Pf6N2E2_104 overlap by 1 nucleotides
Pf6N2E2_102 - sigma-54 dependent transcriptional regulator, at 146,344 to 148,188
_102
Pf6N2E2_103 - Various polyols ABC transporter, ATP-binding component, at 148,544 to 149,638
_103
Pf6N2E2_104 - Glycerol-3-phosphate ABC transporter, ATP-binding protein UgpC (TC 3.A.1.1.3), at 149,638 to 150,747
_104
Position (kb) 148
149
150 Strain fitness (log2 ratio) -1
0
1 at 147.686 kb on + strand, within Pf6N2E2_102 at 147.686 kb on + strand, within Pf6N2E2_102 at 147.687 kb on - strand, within Pf6N2E2_102 at 147.687 kb on - strand, within Pf6N2E2_102 at 147.687 kb on - strand, within Pf6N2E2_102 at 148.214 kb on + strand at 148.214 kb on + strand at 148.265 kb on + strand at 148.275 kb on + strand at 148.275 kb on + strand at 148.275 kb on + strand at 148.275 kb on + strand at 148.529 kb on + strand at 148.530 kb on - strand at 148.530 kb on - strand at 148.652 kb on - strand at 149.037 kb on - strand, within Pf6N2E2_103 at 149.037 kb on - strand, within Pf6N2E2_103 at 149.204 kb on + strand, within Pf6N2E2_103 at 149.383 kb on + strand, within Pf6N2E2_103 at 149.383 kb on + strand, within Pf6N2E2_103 at 149.384 kb on - strand, within Pf6N2E2_103 at 149.384 kb on - strand, within Pf6N2E2_103 at 149.525 kb on + strand, within Pf6N2E2_103 at 149.525 kb on + strand, within Pf6N2E2_103 at 149.552 kb on + strand at 149.552 kb on + strand at 149.553 kb on - strand at 149.675 kb on - strand at 149.675 kb on - strand at 149.914 kb on + strand, within Pf6N2E2_104 at 149.914 kb on + strand, within Pf6N2E2_104 at 149.914 kb on + strand, within Pf6N2E2_104 at 149.915 kb on - strand, within Pf6N2E2_104 at 149.915 kb on - strand, within Pf6N2E2_104 at 149.915 kb on - strand, within Pf6N2E2_104 at 149.942 kb on + strand, within Pf6N2E2_104 at 149.943 kb on - strand, within Pf6N2E2_104 at 149.943 kb on - strand, within Pf6N2E2_104 at 150.223 kb on + strand, within Pf6N2E2_104 at 150.224 kb on - strand, within Pf6N2E2_104 at 150.280 kb on + strand, within Pf6N2E2_104 at 150.281 kb on - strand, within Pf6N2E2_104 at 150.281 kb on - strand, within Pf6N2E2_104 at 150.532 kb on + strand, within Pf6N2E2_104 at 150.533 kb on - strand, within Pf6N2E2_104 at 150.583 kb on + strand, within Pf6N2E2_104 at 150.583 kb on + strand, within Pf6N2E2_104 at 150.584 kb on - strand, within Pf6N2E2_104 at 150.584 kb on - strand, within Pf6N2E2_104 at 150.627 kb on - strand, within Pf6N2E2_104
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 147,686 + Pf6N2E2_102 0.73 +0.1 147,686 + Pf6N2E2_102 0.73 -0.2 147,687 - Pf6N2E2_102 0.73 +0.3 147,687 - Pf6N2E2_102 0.73 -0.0 147,687 - Pf6N2E2_102 0.73 -0.1 148,214 + -0.7 148,214 + -0.3 148,265 + -0.6 148,275 + +0.6 148,275 + +0.9 148,275 + -0.9 148,275 + +0.9 148,529 + +0.0 148,530 - -1.0 148,530 - -0.3 148,652 - +0.4 149,037 - Pf6N2E2_103 0.45 +0.1 149,037 - Pf6N2E2_103 0.45 +0.2 149,204 + Pf6N2E2_103 0.60 -0.0 149,383 + Pf6N2E2_103 0.77 -0.8 149,383 + Pf6N2E2_103 0.77 -0.1 149,384 - Pf6N2E2_103 0.77 +0.6 149,384 - Pf6N2E2_103 0.77 +0.5 149,525 + Pf6N2E2_103 0.90 +1.0 149,525 + Pf6N2E2_103 0.90 +1.1 149,552 + +0.1 149,552 + -1.3 149,553 - -0.4 149,675 - -1.6 149,675 - +0.0 149,914 + Pf6N2E2_104 0.25 -0.4 149,914 + Pf6N2E2_104 0.25 -0.1 149,914 + Pf6N2E2_104 0.25 +0.2 149,915 - Pf6N2E2_104 0.25 +0.3 149,915 - Pf6N2E2_104 0.25 -0.5 149,915 - Pf6N2E2_104 0.25 -0.1 149,942 + Pf6N2E2_104 0.27 -0.7 149,943 - Pf6N2E2_104 0.27 +0.6 149,943 - Pf6N2E2_104 0.27 +0.2 150,223 + Pf6N2E2_104 0.53 -0.4 150,224 - Pf6N2E2_104 0.53 +0.1 150,280 + Pf6N2E2_104 0.58 +0.6 150,281 - Pf6N2E2_104 0.58 -1.0 150,281 - Pf6N2E2_104 0.58 +0.2 150,532 + Pf6N2E2_104 0.81 -0.4 150,533 - Pf6N2E2_104 0.81 -0.0 150,583 + Pf6N2E2_104 0.85 -1.3 150,583 + Pf6N2E2_104 0.85 -0.3 150,584 - Pf6N2E2_104 0.85 +0.6 150,584 - Pf6N2E2_104 0.85 -0.7 150,627 - Pf6N2E2_104 0.89 +0.4
Or see this region's nucleotide sequence