Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4532 and Pf6N2E2_4533 overlap by 14 nucleotides Pf6N2E2_4533 and Pf6N2E2_4534 are separated by 115 nucleotides Pf6N2E2_4534 and Pf6N2E2_4535 overlap by 4 nucleotides Pf6N2E2_4535 and Pf6N2E2_4536 overlap by 4 nucleotides Pf6N2E2_4536 and Pf6N2E2_4537 are separated by 9 nucleotides Pf6N2E2_4537 and Pf6N2E2_4538 overlap by 1 nucleotides
Pf6N2E2_4532 - Flagellum-specific ATP synthase FliI, at 5,266,996 to 5,268,351
_4532
Pf6N2E2_4533 - RspO, at 5,268,338 to 5,268,757
_4533
Pf6N2E2_4534 - hypothetical protein, at 5,268,873 to 5,269,319
_4534
Pf6N2E2_4535 - Type III secretion inner membrane protein (YscQ,homologous to flagellar export components), at 5,269,316 to 5,270,419
_4535
Pf6N2E2_4536 - Type III secretion inner membrane protein (YscR,SpaR,HrcR,EscR,homologous to flagellar export components), at 5,270,416 to 5,271,069
_4536
Pf6N2E2_4537 - Type III secretion inner membrane protein (YscS,homologous to flagellar export components), at 5,271,079 to 5,271,333
_4537
Pf6N2E2_4538 - Type III secretion inner membrane protein (YscT,HrcT,SpaR,EscT,EpaR1,homologous to flagellar export components), at 5,271,333 to 5,272,130
_4538
Position (kb) 5269
5270
5271 Strain fitness (log2 ratio) -1
0
1 at 5268.337 kb on + strand at 5268.337 kb on + strand at 5268.337 kb on + strand at 5268.338 kb on - strand at 5268.728 kb on + strand at 5268.832 kb on - strand at 5268.939 kb on + strand, within Pf6N2E2_4534 at 5269.208 kb on - strand, within Pf6N2E2_4534 at 5269.208 kb on - strand, within Pf6N2E2_4534 at 5269.208 kb on - strand, within Pf6N2E2_4534 at 5269.501 kb on + strand, within Pf6N2E2_4535 at 5269.502 kb on - strand, within Pf6N2E2_4535 at 5269.502 kb on - strand, within Pf6N2E2_4535 at 5269.502 kb on - strand, within Pf6N2E2_4535 at 5269.502 kb on - strand, within Pf6N2E2_4535 at 5269.645 kb on + strand, within Pf6N2E2_4535 at 5269.646 kb on - strand, within Pf6N2E2_4535 at 5270.136 kb on - strand, within Pf6N2E2_4535 at 5270.330 kb on + strand at 5270.330 kb on + strand at 5270.331 kb on - strand at 5270.494 kb on - strand, within Pf6N2E2_4536 at 5270.517 kb on - strand, within Pf6N2E2_4536 at 5270.584 kb on + strand, within Pf6N2E2_4536 at 5270.585 kb on - strand, within Pf6N2E2_4536 at 5270.902 kb on + strand, within Pf6N2E2_4536 at 5270.902 kb on + strand, within Pf6N2E2_4536 at 5270.902 kb on + strand, within Pf6N2E2_4536 at 5270.902 kb on + strand, within Pf6N2E2_4536 at 5270.902 kb on + strand, within Pf6N2E2_4536 at 5270.902 kb on + strand, within Pf6N2E2_4536 at 5270.902 kb on + strand, within Pf6N2E2_4536 at 5270.902 kb on + strand, within Pf6N2E2_4536 at 5270.903 kb on - strand, within Pf6N2E2_4536 at 5270.903 kb on - strand, within Pf6N2E2_4536 at 5270.903 kb on - strand, within Pf6N2E2_4536 at 5270.903 kb on - strand, within Pf6N2E2_4536 at 5270.903 kb on - strand, within Pf6N2E2_4536 at 5270.903 kb on - strand, within Pf6N2E2_4536 at 5270.903 kb on - strand, within Pf6N2E2_4536 at 5271.055 kb on + strand at 5271.055 kb on + strand at 5271.055 kb on + strand at 5271.055 kb on + strand at 5271.056 kb on - strand at 5271.056 kb on - strand at 5271.056 kb on - strand at 5271.056 kb on - strand at 5271.056 kb on - strand at 5271.061 kb on + strand at 5271.061 kb on + strand at 5271.061 kb on + strand at 5271.100 kb on - strand at 5271.167 kb on + strand, within Pf6N2E2_4537 at 5271.167 kb on + strand, within Pf6N2E2_4537 at 5271.328 kb on - strand at 5271.357 kb on + strand at 5271.403 kb on - strand at 5271.414 kb on + strand, within Pf6N2E2_4538
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 5,268,337 + +1.3 5,268,337 + -0.5 5,268,337 + +0.7 5,268,338 - +0.2 5,268,728 + +1.4 5,268,832 - -0.3 5,268,939 + Pf6N2E2_4534 0.15 +0.7 5,269,208 - Pf6N2E2_4534 0.75 +0.7 5,269,208 - Pf6N2E2_4534 0.75 -0.4 5,269,208 - Pf6N2E2_4534 0.75 +0.3 5,269,501 + Pf6N2E2_4535 0.17 -0.2 5,269,502 - Pf6N2E2_4535 0.17 -0.6 5,269,502 - Pf6N2E2_4535 0.17 -0.4 5,269,502 - Pf6N2E2_4535 0.17 -0.3 5,269,502 - Pf6N2E2_4535 0.17 -1.1 5,269,645 + Pf6N2E2_4535 0.30 -0.3 5,269,646 - Pf6N2E2_4535 0.30 -0.4 5,270,136 - Pf6N2E2_4535 0.74 -0.4 5,270,330 + +0.7 5,270,330 + -0.1 5,270,331 - -0.2 5,270,494 - Pf6N2E2_4536 0.12 -0.2 5,270,517 - Pf6N2E2_4536 0.15 +0.7 5,270,584 + Pf6N2E2_4536 0.26 +0.1 5,270,585 - Pf6N2E2_4536 0.26 +0.2 5,270,902 + Pf6N2E2_4536 0.74 +0.8 5,270,902 + Pf6N2E2_4536 0.74 +0.9 5,270,902 + Pf6N2E2_4536 0.74 -0.3 5,270,902 + Pf6N2E2_4536 0.74 -1.4 5,270,902 + Pf6N2E2_4536 0.74 +0.7 5,270,902 + Pf6N2E2_4536 0.74 +0.7 5,270,902 + Pf6N2E2_4536 0.74 -0.4 5,270,902 + Pf6N2E2_4536 0.74 +1.1 5,270,903 - Pf6N2E2_4536 0.74 +0.3 5,270,903 - Pf6N2E2_4536 0.74 -0.8 5,270,903 - Pf6N2E2_4536 0.74 -0.8 5,270,903 - Pf6N2E2_4536 0.74 +0.3 5,270,903 - Pf6N2E2_4536 0.74 -0.2 5,270,903 - Pf6N2E2_4536 0.74 -0.1 5,270,903 - Pf6N2E2_4536 0.74 +0.4 5,271,055 + +0.0 5,271,055 + +0.0 5,271,055 + -1.1 5,271,055 + -0.3 5,271,056 - +0.5 5,271,056 - +0.3 5,271,056 - -0.9 5,271,056 - -0.9 5,271,056 - +0.2 5,271,061 + +0.9 5,271,061 + -0.2 5,271,061 + +0.2 5,271,100 - -0.3 5,271,167 + Pf6N2E2_4537 0.35 -0.0 5,271,167 + Pf6N2E2_4537 0.35 +0.3 5,271,328 - -0.5 5,271,357 + -0.5 5,271,403 - -1.0 5,271,414 + Pf6N2E2_4538 0.10 +0.7
Or see this region's nucleotide sequence