Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_215 and Pf6N2E2_216 are separated by 225 nucleotides Pf6N2E2_216 and Pf6N2E2_217 overlap by 4 nucleotides
Pf6N2E2_215 - Outer membrane protein assembly factor YaeT precursor, at 271,909 to 274,275
_215
Pf6N2E2_216 - Copper-sensing two-component system response regulator CpxR, at 274,501 to 275,184
_216
Pf6N2E2_217 - Signal transduction histidine kinase, at 275,181 to 276,443
_217
Position (kb) 274
275
276 Strain fitness (log2 ratio) -2
-1
0
1 at 274.310 kb on + strand at 274.310 kb on + strand at 274.310 kb on + strand at 274.310 kb on + strand at 274.310 kb on + strand at 274.311 kb on - strand at 274.311 kb on - strand at 274.311 kb on - strand at 274.311 kb on - strand at 274.380 kb on + strand at 274.422 kb on + strand at 274.423 kb on - strand at 274.426 kb on + strand at 274.485 kb on + strand at 274.485 kb on + strand at 274.511 kb on + strand at 274.511 kb on + strand at 274.512 kb on - strand at 274.512 kb on - strand at 274.638 kb on + strand, within Pf6N2E2_216 at 274.638 kb on + strand, within Pf6N2E2_216 at 274.638 kb on + strand, within Pf6N2E2_216 at 274.639 kb on - strand, within Pf6N2E2_216 at 274.640 kb on - strand, within Pf6N2E2_216 at 274.792 kb on + strand, within Pf6N2E2_216 at 274.792 kb on + strand, within Pf6N2E2_216 at 274.792 kb on + strand, within Pf6N2E2_216 at 274.793 kb on - strand, within Pf6N2E2_216 at 275.066 kb on - strand, within Pf6N2E2_216 at 275.164 kb on + strand at 275.322 kb on - strand, within Pf6N2E2_217 at 275.335 kb on + strand, within Pf6N2E2_217 at 275.335 kb on + strand, within Pf6N2E2_217 at 275.336 kb on - strand, within Pf6N2E2_217 at 275.336 kb on - strand, within Pf6N2E2_217 at 275.415 kb on + strand, within Pf6N2E2_217 at 275.467 kb on - strand, within Pf6N2E2_217 at 275.607 kb on + strand, within Pf6N2E2_217 at 275.607 kb on + strand, within Pf6N2E2_217 at 275.620 kb on - strand, within Pf6N2E2_217 at 275.779 kb on + strand, within Pf6N2E2_217
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 274,310 + +0.4 274,310 + -0.3 274,310 + -0.3 274,310 + +0.9 274,310 + -0.2 274,311 - -2.1 274,311 - +0.9 274,311 - -0.6 274,311 - +0.4 274,380 + -1.4 274,422 + -0.7 274,423 - -0.1 274,426 + -1.1 274,485 + -0.1 274,485 + -0.2 274,511 + -0.3 274,511 + -0.7 274,512 - +1.1 274,512 - +0.8 274,638 + Pf6N2E2_216 0.20 -0.7 274,638 + Pf6N2E2_216 0.20 +1.3 274,638 + Pf6N2E2_216 0.20 -0.2 274,639 - Pf6N2E2_216 0.20 -0.8 274,640 - Pf6N2E2_216 0.20 -0.9 274,792 + Pf6N2E2_216 0.43 -0.5 274,792 + Pf6N2E2_216 0.43 -0.9 274,792 + Pf6N2E2_216 0.43 -0.9 274,793 - Pf6N2E2_216 0.43 +0.2 275,066 - Pf6N2E2_216 0.83 -0.3 275,164 + +0.5 275,322 - Pf6N2E2_217 0.11 +0.1 275,335 + Pf6N2E2_217 0.12 -1.4 275,335 + Pf6N2E2_217 0.12 -0.6 275,336 - Pf6N2E2_217 0.12 -1.5 275,336 - Pf6N2E2_217 0.12 +1.0 275,415 + Pf6N2E2_217 0.19 +0.3 275,467 - Pf6N2E2_217 0.23 +0.5 275,607 + Pf6N2E2_217 0.34 -1.5 275,607 + Pf6N2E2_217 0.34 -0.2 275,620 - Pf6N2E2_217 0.35 +0.3 275,779 + Pf6N2E2_217 0.47 +0.5
Or see this region's nucleotide sequence