Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_694 and Pf6N2E2_695 are separated by 99 nucleotides Pf6N2E2_695 and Pf6N2E2_696 overlap by 4 nucleotides Pf6N2E2_696 and Pf6N2E2_697 are separated by 40 nucleotides
Pf6N2E2_694 - Outer membrane protein (porin), at 800,120 to 801,415
_694
Pf6N2E2_695 - Nitrite reductase associated c-type cytochorome NirN, at 801,515 to 803,011
_695
Pf6N2E2_696 - Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 803,008 to 803,850
_696
Pf6N2E2_697 - Heme d1 biosynthesis protein NirJ, at 803,891 to 805,069
_697
Position (kb) 801
802
803
804 Strain fitness (log2 ratio) -1
0
1
2 at 800.814 kb on - strand, within Pf6N2E2_694 at 800.843 kb on + strand, within Pf6N2E2_694 at 800.843 kb on + strand, within Pf6N2E2_694 at 800.844 kb on - strand, within Pf6N2E2_694 at 800.844 kb on - strand, within Pf6N2E2_694 at 800.844 kb on - strand, within Pf6N2E2_694 at 800.864 kb on + strand, within Pf6N2E2_694 at 800.925 kb on - strand, within Pf6N2E2_694 at 801.051 kb on - strand, within Pf6N2E2_694 at 801.068 kb on + strand, within Pf6N2E2_694 at 801.068 kb on + strand, within Pf6N2E2_694 at 801.068 kb on + strand, within Pf6N2E2_694 at 801.485 kb on + strand at 801.561 kb on + strand at 801.561 kb on + strand at 801.562 kb on - strand at 801.562 kb on - strand at 801.562 kb on - strand at 801.807 kb on + strand, within Pf6N2E2_695 at 801.957 kb on + strand, within Pf6N2E2_695 at 801.958 kb on - strand, within Pf6N2E2_695 at 802.124 kb on - strand, within Pf6N2E2_695 at 802.224 kb on + strand, within Pf6N2E2_695 at 802.432 kb on - strand, within Pf6N2E2_695 at 802.726 kb on - strand, within Pf6N2E2_695 at 802.917 kb on + strand at 802.917 kb on + strand at 802.917 kb on + strand at 802.917 kb on + strand at 802.918 kb on - strand at 802.918 kb on - strand at 802.982 kb on + strand at 802.982 kb on + strand at 802.983 kb on - strand at 802.985 kb on + strand at 802.985 kb on + strand at 802.985 kb on + strand at 802.986 kb on - strand at 802.986 kb on - strand at 802.986 kb on - strand at 803.232 kb on + strand, within Pf6N2E2_696 at 803.232 kb on + strand, within Pf6N2E2_696 at 803.297 kb on + strand, within Pf6N2E2_696 at 803.297 kb on + strand, within Pf6N2E2_696 at 803.297 kb on + strand, within Pf6N2E2_696 at 803.297 kb on + strand, within Pf6N2E2_696 at 803.429 kb on + strand, within Pf6N2E2_696 at 803.429 kb on + strand, within Pf6N2E2_696 at 803.430 kb on - strand, within Pf6N2E2_696 at 803.430 kb on - strand, within Pf6N2E2_696 at 803.499 kb on - strand, within Pf6N2E2_696 at 803.646 kb on - strand, within Pf6N2E2_696 at 803.705 kb on + strand, within Pf6N2E2_696 at 803.706 kb on - strand, within Pf6N2E2_696 at 803.706 kb on - strand, within Pf6N2E2_696 at 803.706 kb on - strand, within Pf6N2E2_696
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 800,814 - Pf6N2E2_694 0.54 -0.2 800,843 + Pf6N2E2_694 0.56 +1.0 800,843 + Pf6N2E2_694 0.56 +0.5 800,844 - Pf6N2E2_694 0.56 -1.7 800,844 - Pf6N2E2_694 0.56 -1.1 800,844 - Pf6N2E2_694 0.56 -1.0 800,864 + Pf6N2E2_694 0.57 -0.1 800,925 - Pf6N2E2_694 0.62 -0.6 801,051 - Pf6N2E2_694 0.72 +1.0 801,068 + Pf6N2E2_694 0.73 +0.4 801,068 + Pf6N2E2_694 0.73 -0.2 801,068 + Pf6N2E2_694 0.73 +0.2 801,485 + +0.5 801,561 + +0.6 801,561 + -0.1 801,562 - -0.9 801,562 - +0.1 801,562 - -1.5 801,807 + Pf6N2E2_695 0.20 +0.2 801,957 + Pf6N2E2_695 0.30 +1.1 801,958 - Pf6N2E2_695 0.30 -0.4 802,124 - Pf6N2E2_695 0.41 +0.2 802,224 + Pf6N2E2_695 0.47 -0.1 802,432 - Pf6N2E2_695 0.61 +0.2 802,726 - Pf6N2E2_695 0.81 +1.0 802,917 + +0.6 802,917 + -0.3 802,917 + +0.5 802,917 + +0.1 802,918 - +0.2 802,918 - -0.4 802,982 + -0.7 802,982 + +0.9 802,983 - +0.1 802,985 + +0.2 802,985 + -1.4 802,985 + +0.1 802,986 - +0.3 802,986 - +0.5 802,986 - +0.3 803,232 + Pf6N2E2_696 0.27 +1.2 803,232 + Pf6N2E2_696 0.27 -1.0 803,297 + Pf6N2E2_696 0.34 +0.4 803,297 + Pf6N2E2_696 0.34 +0.6 803,297 + Pf6N2E2_696 0.34 -0.4 803,297 + Pf6N2E2_696 0.34 +0.1 803,429 + Pf6N2E2_696 0.50 +1.0 803,429 + Pf6N2E2_696 0.50 -1.4 803,430 - Pf6N2E2_696 0.50 +2.3 803,430 - Pf6N2E2_696 0.50 +1.0 803,499 - Pf6N2E2_696 0.58 +0.7 803,646 - Pf6N2E2_696 0.76 -0.7 803,705 + Pf6N2E2_696 0.83 +0.3 803,706 - Pf6N2E2_696 0.83 -0.4 803,706 - Pf6N2E2_696 0.83 -0.1 803,706 - Pf6N2E2_696 0.83 +0.1
Or see this region's nucleotide sequence