Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_328

Experiment: LB no stress 24 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_326 and Pf6N2E2_327 are separated by 18 nucleotidesPf6N2E2_327 and Pf6N2E2_328 are separated by 246 nucleotidesPf6N2E2_328 and Pf6N2E2_329 are separated by 161 nucleotides Pf6N2E2_326 - Putative nucleoside transporter YegT, at 394,042 to 395,274 _326 Pf6N2E2_327 - Alginate lyase precursor (EC 4.2.2.3), at 395,293 to 395,949 _327 Pf6N2E2_328 - hypothetical protein, at 396,196 to 396,531 _328 Pf6N2E2_329 - L-lactate permease, at 396,693 to 398,456 _329 Position (kb) 396 397Strain fitness (log2 ratio) -1 0 1at 395.229 kb on + strandat 395.229 kb on + strandat 395.230 kb on - strandat 395.321 kb on + strandat 395.322 kb on - strandat 395.387 kb on + strand, within Pf6N2E2_327at 395.387 kb on + strand, within Pf6N2E2_327at 395.388 kb on - strand, within Pf6N2E2_327at 395.388 kb on - strand, within Pf6N2E2_327at 395.394 kb on + strand, within Pf6N2E2_327at 395.394 kb on + strand, within Pf6N2E2_327at 395.394 kb on + strand, within Pf6N2E2_327at 395.395 kb on - strand, within Pf6N2E2_327at 395.395 kb on - strand, within Pf6N2E2_327at 395.395 kb on - strand, within Pf6N2E2_327at 395.396 kb on + strand, within Pf6N2E2_327at 395.397 kb on - strand, within Pf6N2E2_327at 395.397 kb on - strand, within Pf6N2E2_327at 395.397 kb on - strand, within Pf6N2E2_327at 395.486 kb on + strand, within Pf6N2E2_327at 395.487 kb on - strand, within Pf6N2E2_327at 395.487 kb on - strand, within Pf6N2E2_327at 395.525 kb on + strand, within Pf6N2E2_327at 395.526 kb on - strand, within Pf6N2E2_327at 395.526 kb on - strand, within Pf6N2E2_327at 395.526 kb on - strand, within Pf6N2E2_327at 395.526 kb on - strand, within Pf6N2E2_327at 395.526 kb on - strand, within Pf6N2E2_327at 395.564 kb on + strand, within Pf6N2E2_327at 395.625 kb on + strand, within Pf6N2E2_327at 395.855 kb on + strand, within Pf6N2E2_327at 395.855 kb on + strand, within Pf6N2E2_327at 395.856 kb on - strand, within Pf6N2E2_327at 395.856 kb on - strand, within Pf6N2E2_327at 395.952 kb on + strandat 395.952 kb on + strandat 395.952 kb on + strandat 395.952 kb on + strandat 396.112 kb on + strandat 396.146 kb on + strandat 396.146 kb on + strandat 396.146 kb on + strandat 396.283 kb on + strand, within Pf6N2E2_328at 396.283 kb on + strand, within Pf6N2E2_328at 396.283 kb on + strand, within Pf6N2E2_328at 396.284 kb on - strand, within Pf6N2E2_328at 396.298 kb on + strand, within Pf6N2E2_328at 396.298 kb on + strand, within Pf6N2E2_328at 396.482 kb on - strand, within Pf6N2E2_328at 396.547 kb on + strandat 396.547 kb on + strandat 396.547 kb on + strandat 396.548 kb on - strandat 396.548 kb on - strandat 396.548 kb on - strandat 396.548 kb on - strandat 396.548 kb on - strandat 396.565 kb on - strandat 396.574 kb on + strandat 396.574 kb on + strandat 396.713 kb on + strandat 396.714 kb on - strandat 396.714 kb on - strandat 396.714 kb on - strandat 396.714 kb on - strandat 396.714 kb on - strandat 396.714 kb on - strandat 396.714 kb on - strandat 396.733 kb on + strandat 396.733 kb on + strandat 396.734 kb on - strandat 396.734 kb on - strandat 396.734 kb on - strandat 396.734 kb on - strandat 396.734 kb on - strandat 396.734 kb on - strandat 397.126 kb on - strand, within Pf6N2E2_329at 397.126 kb on - strand, within Pf6N2E2_329at 397.126 kb on - strand, within Pf6N2E2_329at 397.134 kb on - strand, within Pf6N2E2_329at 397.134 kb on - strand, within Pf6N2E2_329at 397.171 kb on + strand, within Pf6N2E2_329at 397.172 kb on - strand, within Pf6N2E2_329at 397.199 kb on - strand, within Pf6N2E2_329at 397.199 kb on - strand, within Pf6N2E2_329at 397.274 kb on - strand, within Pf6N2E2_329at 397.292 kb on + strand, within Pf6N2E2_329

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Per-strain Table

Position Strand Gene LocusTag Fraction LB no stress 24 hours
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395,229 + -0.7
395,229 + +0.2
395,230 - +0.1
395,321 + +1.4
395,322 - -0.6
395,387 + Pf6N2E2_327 0.14 +0.4
395,387 + Pf6N2E2_327 0.14 +1.2
395,388 - Pf6N2E2_327 0.14 +0.0
395,388 - Pf6N2E2_327 0.14 +0.2
395,394 + Pf6N2E2_327 0.15 -1.0
395,394 + Pf6N2E2_327 0.15 -0.1
395,394 + Pf6N2E2_327 0.15 -1.4
395,395 - Pf6N2E2_327 0.16 +0.2
395,395 - Pf6N2E2_327 0.16 -0.5
395,395 - Pf6N2E2_327 0.16 +0.3
395,396 + Pf6N2E2_327 0.16 -0.0
395,397 - Pf6N2E2_327 0.16 -0.3
395,397 - Pf6N2E2_327 0.16 +0.1
395,397 - Pf6N2E2_327 0.16 +0.7
395,486 + Pf6N2E2_327 0.29 +0.2
395,487 - Pf6N2E2_327 0.30 +1.1
395,487 - Pf6N2E2_327 0.30 +0.3
395,525 + Pf6N2E2_327 0.35 +0.4
395,526 - Pf6N2E2_327 0.35 +0.1
395,526 - Pf6N2E2_327 0.35 +0.5
395,526 - Pf6N2E2_327 0.35 -1.4
395,526 - Pf6N2E2_327 0.35 +0.8
395,526 - Pf6N2E2_327 0.35 -0.1
395,564 + Pf6N2E2_327 0.41 -0.9
395,625 + Pf6N2E2_327 0.51 -0.5
395,855 + Pf6N2E2_327 0.86 -1.7
395,855 + Pf6N2E2_327 0.86 +0.8
395,856 - Pf6N2E2_327 0.86 +0.6
395,856 - Pf6N2E2_327 0.86 +0.1
395,952 + -0.5
395,952 + +0.1
395,952 + -0.4
395,952 + -0.5
396,112 + +0.2
396,146 + +0.7
396,146 + -0.2
396,146 + +0.1
396,283 + Pf6N2E2_328 0.26 -0.1
396,283 + Pf6N2E2_328 0.26 +1.4
396,283 + Pf6N2E2_328 0.26 +0.4
396,284 - Pf6N2E2_328 0.26 +1.0
396,298 + Pf6N2E2_328 0.30 +0.4
396,298 + Pf6N2E2_328 0.30 +0.1
396,482 - Pf6N2E2_328 0.85 +0.5
396,547 + -0.1
396,547 + +0.5
396,547 + +0.0
396,548 - -1.5
396,548 - +0.2
396,548 - -0.8
396,548 - -0.6
396,548 - +0.2
396,565 - -0.4
396,574 + -0.5
396,574 + -0.4
396,713 + -0.3
396,714 - -0.5
396,714 - +0.1
396,714 - +0.3
396,714 - +0.2
396,714 - +0.8
396,714 - +0.0
396,714 - -0.2
396,733 + +0.1
396,733 + +0.3
396,734 - +0.2
396,734 - +0.3
396,734 - -0.3
396,734 - +0.4
396,734 - -1.3
396,734 - -1.1
397,126 - Pf6N2E2_329 0.25 -0.5
397,126 - Pf6N2E2_329 0.25 +0.1
397,126 - Pf6N2E2_329 0.25 +1.3
397,134 - Pf6N2E2_329 0.25 -0.2
397,134 - Pf6N2E2_329 0.25 +0.9
397,171 + Pf6N2E2_329 0.27 -0.4
397,172 - Pf6N2E2_329 0.27 -0.7
397,199 - Pf6N2E2_329 0.29 -0.1
397,199 - Pf6N2E2_329 0.29 +0.6
397,274 - Pf6N2E2_329 0.33 +0.3
397,292 + Pf6N2E2_329 0.34 +0.3

Or see this region's nucleotide sequence