Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1954 and Pf6N2E2_1955 are separated by 477 nucleotides Pf6N2E2_1955 and Pf6N2E2_1956 are separated by 49 nucleotides
Pf6N2E2_1954 - Transcriptional regulator, LysR family, at 2,318,595 to 2,319,482
_1954
Pf6N2E2_1955 - daunorubicin C-13 ketoreductase XF1741, at 2,319,960 to 2,320,559
_1955
Pf6N2E2_1956 - Outer membrane protein romA, at 2,320,609 to 2,321,745
_1956
Position (kb)
2319
2320
2321 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2319.091 kb on + strand, within Pf6N2E2_1954 at 2319.189 kb on - strand, within Pf6N2E2_1954 at 2319.191 kb on + strand, within Pf6N2E2_1954 at 2319.192 kb on - strand, within Pf6N2E2_1954 at 2319.279 kb on + strand, within Pf6N2E2_1954 at 2319.297 kb on + strand, within Pf6N2E2_1954 at 2319.302 kb on + strand, within Pf6N2E2_1954 at 2319.302 kb on + strand, within Pf6N2E2_1954 at 2319.303 kb on - strand, within Pf6N2E2_1954 at 2319.303 kb on - strand, within Pf6N2E2_1954 at 2319.303 kb on - strand, within Pf6N2E2_1954 at 2319.309 kb on + strand, within Pf6N2E2_1954 at 2319.310 kb on - strand, within Pf6N2E2_1954 at 2319.330 kb on - strand, within Pf6N2E2_1954 at 2319.399 kb on + strand at 2319.399 kb on + strand at 2319.403 kb on - strand at 2319.453 kb on + strand at 2319.453 kb on + strand at 2319.511 kb on - strand at 2319.521 kb on - strand at 2319.569 kb on + strand at 2319.570 kb on - strand at 2319.570 kb on - strand at 2319.570 kb on - strand at 2319.574 kb on + strand at 2319.575 kb on - strand at 2319.601 kb on + strand at 2319.601 kb on + strand at 2319.603 kb on + strand at 2319.603 kb on + strand at 2319.603 kb on + strand at 2319.678 kb on - strand at 2319.678 kb on - strand at 2319.818 kb on + strand at 2319.818 kb on + strand at 2319.818 kb on + strand at 2319.819 kb on - strand at 2320.099 kb on + strand, within Pf6N2E2_1955 at 2320.099 kb on + strand, within Pf6N2E2_1955 at 2320.100 kb on - strand, within Pf6N2E2_1955 at 2320.122 kb on + strand, within Pf6N2E2_1955 at 2320.122 kb on + strand, within Pf6N2E2_1955 at 2320.123 kb on - strand, within Pf6N2E2_1955 at 2320.123 kb on - strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.125 kb on - strand, within Pf6N2E2_1955 at 2320.125 kb on - strand, within Pf6N2E2_1955 at 2320.125 kb on - strand, within Pf6N2E2_1955 at 2320.125 kb on - strand, within Pf6N2E2_1955 at 2320.125 kb on - strand, within Pf6N2E2_1955 at 2320.164 kb on + strand, within Pf6N2E2_1955 at 2320.164 kb on + strand, within Pf6N2E2_1955 at 2320.165 kb on - strand, within Pf6N2E2_1955 at 2320.165 kb on - strand, within Pf6N2E2_1955 at 2320.165 kb on - strand, within Pf6N2E2_1955 at 2320.185 kb on - strand, within Pf6N2E2_1955 at 2320.246 kb on - strand, within Pf6N2E2_1955 at 2320.316 kb on + strand, within Pf6N2E2_1955 at 2320.317 kb on - strand, within Pf6N2E2_1955 at 2320.573 kb on - strand at 2320.576 kb on + strand at 2320.577 kb on - strand at 2320.577 kb on - strand at 2320.577 kb on - strand at 2320.577 kb on - strand at 2320.577 kb on - strand at 2320.590 kb on - strand at 2320.635 kb on - strand at 2320.657 kb on + strand at 2320.658 kb on - strand at 2320.661 kb on + strand at 2320.661 kb on + strand at 2320.662 kb on - strand at 2320.662 kb on - strand at 2320.662 kb on - strand at 2320.662 kb on - strand at 2320.662 kb on - strand at 2320.891 kb on - strand, within Pf6N2E2_1956 at 2320.899 kb on + strand, within Pf6N2E2_1956 at 2320.899 kb on + strand, within Pf6N2E2_1956 at 2320.900 kb on - strand, within Pf6N2E2_1956 at 2320.900 kb on - strand, within Pf6N2E2_1956 at 2320.901 kb on + strand, within Pf6N2E2_1956 at 2320.902 kb on - strand, within Pf6N2E2_1956 at 2320.928 kb on - strand, within Pf6N2E2_1956 at 2320.981 kb on + strand, within Pf6N2E2_1956 at 2320.981 kb on + strand, within Pf6N2E2_1956 at 2320.982 kb on - strand, within Pf6N2E2_1956 at 2321.049 kb on - strand, within Pf6N2E2_1956 at 2321.087 kb on - strand, within Pf6N2E2_1956 at 2321.102 kb on - strand, within Pf6N2E2_1956 at 2321.159 kb on + strand, within Pf6N2E2_1956 at 2321.160 kb on - strand, within Pf6N2E2_1956 at 2321.160 kb on - strand, within Pf6N2E2_1956 at 2321.276 kb on - strand, within Pf6N2E2_1956 at 2321.276 kb on - strand, within Pf6N2E2_1956 at 2321.419 kb on + strand, within Pf6N2E2_1956 at 2321.443 kb on + strand, within Pf6N2E2_1956 at 2321.443 kb on + strand, within Pf6N2E2_1956 at 2321.443 kb on + strand, within Pf6N2E2_1956
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 2,319,091 + Pf6N2E2_1954 0.56 -0.5 2,319,189 - Pf6N2E2_1954 0.67 -2.6 2,319,191 + Pf6N2E2_1954 0.67 -3.1 2,319,192 - Pf6N2E2_1954 0.67 +1.7 2,319,279 + Pf6N2E2_1954 0.77 -0.1 2,319,297 + Pf6N2E2_1954 0.79 -1.1 2,319,302 + Pf6N2E2_1954 0.80 -0.3 2,319,302 + Pf6N2E2_1954 0.80 -0.1 2,319,303 - Pf6N2E2_1954 0.80 +0.6 2,319,303 - Pf6N2E2_1954 0.80 +0.1 2,319,303 - Pf6N2E2_1954 0.80 -0.5 2,319,309 + Pf6N2E2_1954 0.80 -0.6 2,319,310 - Pf6N2E2_1954 0.81 -0.0 2,319,330 - Pf6N2E2_1954 0.83 -2.7 2,319,399 + +0.2 2,319,399 + -1.0 2,319,403 - +0.4 2,319,453 + -0.2 2,319,453 + -0.1 2,319,511 - +0.1 2,319,521 - -0.3 2,319,569 + +0.1 2,319,570 - +0.2 2,319,570 - -0.0 2,319,570 - -0.9 2,319,574 + -1.3 2,319,575 - +0.3 2,319,601 + -0.5 2,319,601 + +0.2 2,319,603 + -0.7 2,319,603 + +0.5 2,319,603 + -0.3 2,319,678 - -1.2 2,319,678 - -1.3 2,319,818 + -1.3 2,319,818 + -0.8 2,319,818 + +0.8 2,319,819 - -0.9 2,320,099 + Pf6N2E2_1955 0.23 +0.1 2,320,099 + Pf6N2E2_1955 0.23 +0.9 2,320,100 - Pf6N2E2_1955 0.23 -0.4 2,320,122 + Pf6N2E2_1955 0.27 +1.2 2,320,122 + Pf6N2E2_1955 0.27 -0.2 2,320,123 - Pf6N2E2_1955 0.27 +0.8 2,320,123 - Pf6N2E2_1955 0.27 +0.2 2,320,124 + Pf6N2E2_1955 0.27 +0.5 2,320,124 + Pf6N2E2_1955 0.27 -0.7 2,320,124 + Pf6N2E2_1955 0.27 -0.5 2,320,124 + Pf6N2E2_1955 0.27 +0.6 2,320,124 + Pf6N2E2_1955 0.27 +0.6 2,320,124 + Pf6N2E2_1955 0.27 -0.4 2,320,124 + Pf6N2E2_1955 0.27 +1.2 2,320,125 - Pf6N2E2_1955 0.28 -0.1 2,320,125 - Pf6N2E2_1955 0.28 +0.3 2,320,125 - Pf6N2E2_1955 0.28 -0.2 2,320,125 - Pf6N2E2_1955 0.28 -0.2 2,320,125 - Pf6N2E2_1955 0.28 +0.9 2,320,164 + Pf6N2E2_1955 0.34 -0.0 2,320,164 + Pf6N2E2_1955 0.34 -0.9 2,320,165 - Pf6N2E2_1955 0.34 +0.2 2,320,165 - Pf6N2E2_1955 0.34 +1.4 2,320,165 - Pf6N2E2_1955 0.34 +0.1 2,320,185 - Pf6N2E2_1955 0.38 +1.1 2,320,246 - Pf6N2E2_1955 0.48 +0.3 2,320,316 + Pf6N2E2_1955 0.59 +1.6 2,320,317 - Pf6N2E2_1955 0.59 +0.4 2,320,573 - +0.4 2,320,576 + +0.5 2,320,577 - -0.0 2,320,577 - -0.5 2,320,577 - +0.4 2,320,577 - -0.3 2,320,577 - -0.2 2,320,590 - +0.7 2,320,635 - -0.3 2,320,657 + -0.0 2,320,658 - +2.2 2,320,661 + -0.5 2,320,661 + -0.0 2,320,662 - +0.4 2,320,662 - +0.1 2,320,662 - -0.2 2,320,662 - -0.2 2,320,662 - +0.5 2,320,891 - Pf6N2E2_1956 0.25 -0.2 2,320,899 + Pf6N2E2_1956 0.26 +0.3 2,320,899 + Pf6N2E2_1956 0.26 +0.3 2,320,900 - Pf6N2E2_1956 0.26 -0.1 2,320,900 - Pf6N2E2_1956 0.26 -0.1 2,320,901 + Pf6N2E2_1956 0.26 +0.1 2,320,902 - Pf6N2E2_1956 0.26 -1.1 2,320,928 - Pf6N2E2_1956 0.28 +0.6 2,320,981 + Pf6N2E2_1956 0.33 +0.2 2,320,981 + Pf6N2E2_1956 0.33 +0.3 2,320,982 - Pf6N2E2_1956 0.33 -0.2 2,321,049 - Pf6N2E2_1956 0.39 -0.9 2,321,087 - Pf6N2E2_1956 0.42 +0.3 2,321,102 - Pf6N2E2_1956 0.43 +1.2 2,321,159 + Pf6N2E2_1956 0.48 +0.1 2,321,160 - Pf6N2E2_1956 0.48 -0.5 2,321,160 - Pf6N2E2_1956 0.48 +0.2 2,321,276 - Pf6N2E2_1956 0.59 +0.4 2,321,276 - Pf6N2E2_1956 0.59 -1.1 2,321,419 + Pf6N2E2_1956 0.71 +1.1 2,321,443 + Pf6N2E2_1956 0.73 -0.1 2,321,443 + Pf6N2E2_1956 0.73 -1.3 2,321,443 + Pf6N2E2_1956 0.73 +0.1
Or see this region's nucleotide sequence