Experiment: LB no stress 24 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1208 and Pf6N2E2_1209 are separated by 37 nucleotides Pf6N2E2_1209 and Pf6N2E2_1210 are separated by 345 nucleotides Pf6N2E2_1210 and Pf6N2E2_1211 are separated by 125 nucleotides Pf6N2E2_1211 and Pf6N2E2_1212 are separated by 187 nucleotides
Pf6N2E2_1208 - Epoxide hydrolase (EC 3.3.2.9), at 1,414,858 to 1,416,228
_1208
Pf6N2E2_1209 - hypothetical protein, at 1,416,266 to 1,416,523
_1209
Pf6N2E2_1210 - putative aminotransferase, at 1,416,869 to 1,418,017
_1210
Pf6N2E2_1211 - Lactoylglutathione lyase, at 1,418,143 to 1,418,547
_1211
Pf6N2E2_1212 - Oxidoreductase, short chain dehydrogenase/reductase family, at 1,418,735 to 1,419,592
_1212
Position (kb)
1416
1417
1418
1419 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1415.906 kb on + strand, within Pf6N2E2_1208 at 1415.921 kb on + strand, within Pf6N2E2_1208 at 1415.921 kb on + strand, within Pf6N2E2_1208 at 1415.921 kb on + strand at 1415.922 kb on - strand, within Pf6N2E2_1208 at 1415.938 kb on + strand, within Pf6N2E2_1208 at 1416.303 kb on + strand, within Pf6N2E2_1209 at 1416.304 kb on - strand, within Pf6N2E2_1209 at 1416.304 kb on - strand, within Pf6N2E2_1209 at 1416.339 kb on + strand, within Pf6N2E2_1209 at 1416.421 kb on - strand, within Pf6N2E2_1209 at 1416.426 kb on + strand, within Pf6N2E2_1209 at 1416.426 kb on + strand, within Pf6N2E2_1209 at 1416.426 kb on + strand, within Pf6N2E2_1209 at 1416.426 kb on + strand, within Pf6N2E2_1209 at 1416.426 kb on + strand, within Pf6N2E2_1209 at 1416.427 kb on - strand, within Pf6N2E2_1209 at 1416.427 kb on - strand, within Pf6N2E2_1209 at 1416.427 kb on - strand, within Pf6N2E2_1209 at 1416.529 kb on + strand at 1416.530 kb on - strand at 1416.530 kb on - strand at 1416.530 kb on - strand at 1416.662 kb on - strand at 1416.662 kb on - strand at 1416.672 kb on - strand at 1416.672 kb on - strand at 1416.672 kb on - strand at 1416.672 kb on - strand at 1416.779 kb on - strand at 1417.094 kb on + strand, within Pf6N2E2_1210 at 1417.485 kb on + strand, within Pf6N2E2_1210 at 1417.485 kb on + strand, within Pf6N2E2_1210 at 1417.485 kb on + strand, within Pf6N2E2_1210 at 1417.485 kb on + strand, within Pf6N2E2_1210 at 1417.485 kb on + strand, within Pf6N2E2_1210 at 1417.486 kb on - strand, within Pf6N2E2_1210 at 1417.692 kb on - strand, within Pf6N2E2_1210 at 1417.693 kb on + strand, within Pf6N2E2_1210 at 1417.730 kb on + strand, within Pf6N2E2_1210 at 1417.730 kb on + strand, within Pf6N2E2_1210 at 1417.752 kb on - strand, within Pf6N2E2_1210 at 1417.814 kb on + strand, within Pf6N2E2_1210 at 1417.815 kb on - strand, within Pf6N2E2_1210 at 1418.144 kb on + strand at 1418.145 kb on - strand at 1418.145 kb on - strand at 1418.217 kb on - strand, within Pf6N2E2_1211 at 1418.493 kb on - strand, within Pf6N2E2_1211 at 1418.533 kb on + strand at 1418.534 kb on - strand at 1418.534 kb on - strand at 1418.534 kb on - strand at 1418.534 kb on - strand at 1418.573 kb on + strand at 1418.574 kb on - strand at 1418.574 kb on - strand at 1418.574 kb on - strand at 1418.580 kb on - strand at 1418.644 kb on - strand at 1418.646 kb on + strand at 1418.646 kb on + strand at 1418.647 kb on - strand at 1418.647 kb on - strand at 1418.834 kb on + strand, within Pf6N2E2_1212 at 1418.834 kb on + strand, within Pf6N2E2_1212 at 1418.834 kb on + strand, within Pf6N2E2_1212
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 24 hours remove 1,415,906 + Pf6N2E2_1208 0.76 -0.0 1,415,921 + Pf6N2E2_1208 0.78 -1.2 1,415,921 + Pf6N2E2_1208 0.78 +0.5 1,415,921 + +0.8 1,415,922 - Pf6N2E2_1208 0.78 +0.4 1,415,938 + Pf6N2E2_1208 0.79 -0.8 1,416,303 + Pf6N2E2_1209 0.14 -0.1 1,416,304 - Pf6N2E2_1209 0.15 +1.9 1,416,304 - Pf6N2E2_1209 0.15 -0.5 1,416,339 + Pf6N2E2_1209 0.28 +1.0 1,416,421 - Pf6N2E2_1209 0.60 -0.1 1,416,426 + Pf6N2E2_1209 0.62 -0.0 1,416,426 + Pf6N2E2_1209 0.62 +0.6 1,416,426 + Pf6N2E2_1209 0.62 +0.6 1,416,426 + Pf6N2E2_1209 0.62 +1.4 1,416,426 + Pf6N2E2_1209 0.62 -2.0 1,416,427 - Pf6N2E2_1209 0.62 +0.1 1,416,427 - Pf6N2E2_1209 0.62 +0.2 1,416,427 - Pf6N2E2_1209 0.62 +0.0 1,416,529 + +0.1 1,416,530 - -0.4 1,416,530 - +0.1 1,416,530 - -0.6 1,416,662 - -0.5 1,416,662 - +0.4 1,416,672 - -1.1 1,416,672 - -0.2 1,416,672 - +0.1 1,416,672 - +0.6 1,416,779 - +0.7 1,417,094 + Pf6N2E2_1210 0.20 +0.6 1,417,485 + Pf6N2E2_1210 0.54 -0.4 1,417,485 + Pf6N2E2_1210 0.54 +0.9 1,417,485 + Pf6N2E2_1210 0.54 +0.2 1,417,485 + Pf6N2E2_1210 0.54 +0.2 1,417,485 + Pf6N2E2_1210 0.54 +1.3 1,417,486 - Pf6N2E2_1210 0.54 +0.3 1,417,692 - Pf6N2E2_1210 0.72 +0.8 1,417,693 + Pf6N2E2_1210 0.72 +0.6 1,417,730 + Pf6N2E2_1210 0.75 +0.7 1,417,730 + Pf6N2E2_1210 0.75 +0.7 1,417,752 - Pf6N2E2_1210 0.77 +1.2 1,417,814 + Pf6N2E2_1210 0.82 +0.3 1,417,815 - Pf6N2E2_1210 0.82 -1.1 1,418,144 + +1.7 1,418,145 - +0.4 1,418,145 - -1.3 1,418,217 - Pf6N2E2_1211 0.18 -0.2 1,418,493 - Pf6N2E2_1211 0.86 -0.4 1,418,533 + -0.5 1,418,534 - -0.4 1,418,534 - +0.4 1,418,534 - +0.4 1,418,534 - -0.5 1,418,573 + +0.9 1,418,574 - +0.4 1,418,574 - +0.0 1,418,574 - -0.0 1,418,580 - +0.5 1,418,644 - +0.3 1,418,646 + -0.0 1,418,646 + -0.1 1,418,647 - -0.0 1,418,647 - +0.3 1,418,834 + Pf6N2E2_1212 0.12 +0.6 1,418,834 + Pf6N2E2_1212 0.12 -1.2 1,418,834 + Pf6N2E2_1212 0.12 +0.1
Or see this region's nucleotide sequence