Experiment: perchlorate 0.00625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_431 and Pf6N2E2_432 are separated by 1 nucleotides Pf6N2E2_432 and Pf6N2E2_433 are separated by 128 nucleotides Pf6N2E2_433 and Pf6N2E2_434 overlap by 4 nucleotides Pf6N2E2_434 and Pf6N2E2_435 are separated by 15 nucleotides Pf6N2E2_435 and Pf6N2E2_436 overlap by 37 nucleotides
Pf6N2E2_431 - Molybdenum transport system permease protein ModB (TC 3.A.1.8.1), at 498,774 to 499,454
_431
Pf6N2E2_432 - Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1), at 499,456 to 500,208
_432
Pf6N2E2_433 - Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9), at 500,337 to 501,410
_433
Pf6N2E2_434 - hypothetical protein, at 501,407 to 501,544
_434
Pf6N2E2_435 - Probable transcription regulator, at 501,560 to 502,048
_435
Pf6N2E2_436 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 502,012 to 503,085
_436
Position (kb)
500
501
502 Strain fitness (log2 ratio)
-2
-1
0
1 at 499.353 kb on + strand, within Pf6N2E2_431 at 499.354 kb on - strand, within Pf6N2E2_431 at 499.432 kb on + strand at 499.433 kb on - strand at 499.457 kb on + strand at 499.511 kb on + strand at 499.511 kb on + strand at 499.512 kb on - strand at 499.640 kb on + strand, within Pf6N2E2_432 at 499.640 kb on + strand, within Pf6N2E2_432 at 499.640 kb on + strand, within Pf6N2E2_432 at 499.868 kb on + strand, within Pf6N2E2_432 at 499.868 kb on + strand, within Pf6N2E2_432 at 499.868 kb on + strand, within Pf6N2E2_432 at 499.868 kb on + strand, within Pf6N2E2_432 at 499.868 kb on + strand, within Pf6N2E2_432 at 499.869 kb on - strand, within Pf6N2E2_432 at 499.869 kb on - strand, within Pf6N2E2_432 at 499.911 kb on - strand, within Pf6N2E2_432 at 499.911 kb on - strand, within Pf6N2E2_432 at 500.021 kb on + strand, within Pf6N2E2_432 at 500.021 kb on + strand, within Pf6N2E2_432 at 500.021 kb on + strand, within Pf6N2E2_432 at 500.058 kb on + strand, within Pf6N2E2_432 at 500.058 kb on + strand, within Pf6N2E2_432 at 500.160 kb on + strand at 500.160 kb on + strand at 500.160 kb on + strand at 500.161 kb on - strand at 500.208 kb on + strand at 500.208 kb on + strand at 500.208 kb on + strand at 500.208 kb on + strand at 500.208 kb on + strand at 500.208 kb on + strand at 500.209 kb on - strand at 500.209 kb on - strand at 500.209 kb on - strand at 500.209 kb on - strand at 500.235 kb on + strand at 500.235 kb on + strand at 500.237 kb on + strand at 500.238 kb on - strand at 500.238 kb on - strand at 500.240 kb on - strand at 500.247 kb on + strand at 500.248 kb on - strand at 500.249 kb on + strand at 500.249 kb on + strand at 500.250 kb on - strand at 500.250 kb on - strand at 500.250 kb on - strand at 500.251 kb on + strand at 500.251 kb on + strand at 500.252 kb on - strand at 500.677 kb on + strand, within Pf6N2E2_433 at 500.870 kb on - strand, within Pf6N2E2_433 at 501.122 kb on - strand, within Pf6N2E2_433 at 501.122 kb on - strand, within Pf6N2E2_433 at 501.122 kb on - strand, within Pf6N2E2_433 at 501.122 kb on - strand, within Pf6N2E2_433 at 501.448 kb on + strand, within Pf6N2E2_434 at 501.448 kb on + strand, within Pf6N2E2_434 at 501.525 kb on - strand, within Pf6N2E2_434 at 501.540 kb on + strand at 501.541 kb on - strand at 501.541 kb on - strand at 501.743 kb on + strand, within Pf6N2E2_435 at 501.744 kb on - strand, within Pf6N2E2_435 at 501.744 kb on - strand, within Pf6N2E2_435 at 501.744 kb on - strand, within Pf6N2E2_435 at 501.753 kb on - strand, within Pf6N2E2_435 at 501.851 kb on + strand, within Pf6N2E2_435 at 501.851 kb on + strand, within Pf6N2E2_435 at 501.920 kb on + strand, within Pf6N2E2_435 at 501.921 kb on - strand, within Pf6N2E2_435 at 501.975 kb on - strand, within Pf6N2E2_435 at 502.001 kb on + strand at 502.001 kb on + strand at 502.002 kb on - strand at 502.002 kb on - strand at 502.002 kb on - strand at 502.099 kb on + strand at 502.100 kb on - strand at 502.206 kb on - strand, within Pf6N2E2_436 at 502.300 kb on + strand, within Pf6N2E2_436 at 502.301 kb on - strand, within Pf6N2E2_436 at 502.301 kb on - strand, within Pf6N2E2_436
Per-strain Table
Position Strand Gene LocusTag Fraction perchlorate 0.00625 M remove 499,353 + Pf6N2E2_431 0.85 +0.5 499,354 - Pf6N2E2_431 0.85 +0.6 499,432 + +0.0 499,433 - +0.4 499,457 + -0.5 499,511 + +0.2 499,511 + -0.2 499,512 - +0.1 499,640 + Pf6N2E2_432 0.24 -0.7 499,640 + Pf6N2E2_432 0.24 +0.1 499,640 + Pf6N2E2_432 0.24 -0.1 499,868 + Pf6N2E2_432 0.55 -0.8 499,868 + Pf6N2E2_432 0.55 -1.8 499,868 + Pf6N2E2_432 0.55 +0.3 499,868 + Pf6N2E2_432 0.55 -0.9 499,868 + Pf6N2E2_432 0.55 +1.5 499,869 - Pf6N2E2_432 0.55 -0.3 499,869 - Pf6N2E2_432 0.55 +0.9 499,911 - Pf6N2E2_432 0.60 -0.2 499,911 - Pf6N2E2_432 0.60 -1.2 500,021 + Pf6N2E2_432 0.75 +0.5 500,021 + Pf6N2E2_432 0.75 -0.2 500,021 + Pf6N2E2_432 0.75 +0.3 500,058 + Pf6N2E2_432 0.80 -0.3 500,058 + Pf6N2E2_432 0.80 +0.1 500,160 + -0.0 500,160 + -0.1 500,160 + -0.5 500,161 - +0.2 500,208 + -0.2 500,208 + -0.7 500,208 + +0.2 500,208 + +0.9 500,208 + -1.0 500,208 + -0.1 500,209 - +0.1 500,209 - -0.3 500,209 - -0.5 500,209 - -0.5 500,235 + -0.5 500,235 + -0.7 500,237 + -1.0 500,238 - -0.5 500,238 - +0.3 500,240 - -1.6 500,247 + +0.1 500,248 - +0.8 500,249 + -0.0 500,249 + -0.7 500,250 - -0.4 500,250 - +1.3 500,250 - +0.6 500,251 + -0.4 500,251 + +0.7 500,252 - +0.3 500,677 + Pf6N2E2_433 0.32 -1.5 500,870 - Pf6N2E2_433 0.50 -0.5 501,122 - Pf6N2E2_433 0.73 -1.3 501,122 - Pf6N2E2_433 0.73 +0.2 501,122 - Pf6N2E2_433 0.73 -0.2 501,122 - Pf6N2E2_433 0.73 -0.9 501,448 + Pf6N2E2_434 0.30 +0.3 501,448 + Pf6N2E2_434 0.30 +0.1 501,525 - Pf6N2E2_434 0.86 +1.5 501,540 + -1.1 501,541 - +0.4 501,541 - +0.7 501,743 + Pf6N2E2_435 0.37 -0.4 501,744 - Pf6N2E2_435 0.38 +0.0 501,744 - Pf6N2E2_435 0.38 -0.2 501,744 - Pf6N2E2_435 0.38 -0.1 501,753 - Pf6N2E2_435 0.39 +0.2 501,851 + Pf6N2E2_435 0.60 +0.7 501,851 + Pf6N2E2_435 0.60 -0.8 501,920 + Pf6N2E2_435 0.74 +0.2 501,921 - Pf6N2E2_435 0.74 -0.3 501,975 - Pf6N2E2_435 0.85 -0.2 502,001 + +1.3 502,001 + -0.4 502,002 - +0.1 502,002 - -1.0 502,002 - -0.6 502,099 + -0.3 502,100 - +0.8 502,206 - Pf6N2E2_436 0.18 +0.7 502,300 + Pf6N2E2_436 0.27 +0.6 502,301 - Pf6N2E2_436 0.27 +0.0 502,301 - Pf6N2E2_436 0.27 -0.3
Or see this region's nucleotide sequence