Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_663

Experiment: perchlorate 0.00625 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_661 and Pf6N2E2_662 are separated by 242 nucleotidesPf6N2E2_662 and Pf6N2E2_663 are separated by 4 nucleotidesPf6N2E2_663 and Pf6N2E2_664 are separated by 342 nucleotides Pf6N2E2_661 - DipZ protein, at 764,968 to 766,179 _661 Pf6N2E2_662 - Valyl-tRNA synthetase, at 766,422 to 766,739 _662 Pf6N2E2_663 - Transcriptional activator of acetoin dehydrogenase operon AcoR, at 766,744 to 768,609 _663 Pf6N2E2_664 - Acetoin catabolism protein X, at 768,952 to 770,025 _664 Position (kb) 766 767 768 769Strain fitness (log2 ratio) -1 0 1 2at 765.835 kb on + strand, within Pf6N2E2_661at 765.835 kb on + strand, within Pf6N2E2_661at 765.836 kb on - strand, within Pf6N2E2_661at 765.868 kb on + strand, within Pf6N2E2_661at 765.869 kb on - strand, within Pf6N2E2_661at 765.937 kb on + strand, within Pf6N2E2_661at 765.977 kb on + strand, within Pf6N2E2_661at 765.998 kb on - strand, within Pf6N2E2_661at 766.051 kb on + strand, within Pf6N2E2_661at 766.066 kb on + strandat 766.067 kb on - strandat 766.321 kb on + strandat 766.570 kb on - strand, within Pf6N2E2_662at 766.653 kb on + strand, within Pf6N2E2_662at 766.653 kb on + strand, within Pf6N2E2_662at 766.654 kb on - strand, within Pf6N2E2_662at 766.654 kb on - strand, within Pf6N2E2_662at 766.654 kb on - strand, within Pf6N2E2_662at 766.698 kb on + strand, within Pf6N2E2_662at 766.698 kb on + strand, within Pf6N2E2_662at 766.698 kb on + strand, within Pf6N2E2_662at 766.699 kb on - strand, within Pf6N2E2_662at 766.701 kb on + strand, within Pf6N2E2_662at 766.701 kb on + strand, within Pf6N2E2_662at 766.702 kb on - strand, within Pf6N2E2_662at 766.702 kb on - strand, within Pf6N2E2_662at 766.738 kb on - strandat 767.192 kb on + strand, within Pf6N2E2_663at 767.193 kb on - strand, within Pf6N2E2_663at 767.841 kb on - strand, within Pf6N2E2_663at 768.248 kb on + strand, within Pf6N2E2_663at 768.609 kb on + strandat 768.610 kb on - strandat 768.649 kb on + strandat 768.887 kb on - strandat 768.887 kb on - strandat 768.890 kb on - strandat 768.904 kb on + strandat 768.905 kb on - strandat 768.905 kb on - strandat 769.432 kb on + strand, within Pf6N2E2_664at 769.433 kb on - strand, within Pf6N2E2_664at 769.433 kb on - strand, within Pf6N2E2_664

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Per-strain Table

Position Strand Gene LocusTag Fraction perchlorate 0.00625 M
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765,835 + Pf6N2E2_661 0.72 +0.4
765,835 + Pf6N2E2_661 0.72 +0.0
765,836 - Pf6N2E2_661 0.72 -0.0
765,868 + Pf6N2E2_661 0.74 -0.2
765,869 - Pf6N2E2_661 0.74 +0.4
765,937 + Pf6N2E2_661 0.80 -0.3
765,977 + Pf6N2E2_661 0.83 -0.1
765,998 - Pf6N2E2_661 0.85 +0.7
766,051 + Pf6N2E2_661 0.89 -0.3
766,066 + +0.4
766,067 - +0.2
766,321 + -0.0
766,570 - Pf6N2E2_662 0.47 +0.1
766,653 + Pf6N2E2_662 0.73 -1.2
766,653 + Pf6N2E2_662 0.73 +0.8
766,654 - Pf6N2E2_662 0.73 +0.7
766,654 - Pf6N2E2_662 0.73 -0.5
766,654 - Pf6N2E2_662 0.73 -0.4
766,698 + Pf6N2E2_662 0.87 -0.6
766,698 + Pf6N2E2_662 0.87 +0.1
766,698 + Pf6N2E2_662 0.87 +0.4
766,699 - Pf6N2E2_662 0.87 +0.4
766,701 + Pf6N2E2_662 0.88 -0.1
766,701 + Pf6N2E2_662 0.88 -1.3
766,702 - Pf6N2E2_662 0.88 +1.9
766,702 - Pf6N2E2_662 0.88 +0.1
766,738 - -0.1
767,192 + Pf6N2E2_663 0.24 -0.2
767,193 - Pf6N2E2_663 0.24 -0.3
767,841 - Pf6N2E2_663 0.59 -0.2
768,248 + Pf6N2E2_663 0.81 -1.0
768,609 + +0.5
768,610 - +1.1
768,649 + -0.8
768,887 - -1.4
768,887 - +0.1
768,890 - -0.4
768,904 + +0.7
768,905 - -0.4
768,905 - -0.5
769,432 + Pf6N2E2_664 0.45 -0.3
769,433 - Pf6N2E2_664 0.45 +0.4
769,433 - Pf6N2E2_664 0.45 -0.7

Or see this region's nucleotide sequence