Experiment: perchlorate 0.00625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1973 and Pf6N2E2_1974 are separated by 151 nucleotides Pf6N2E2_1974 and Pf6N2E2_1975 overlap by 1 nucleotides Pf6N2E2_1975 and Pf6N2E2_1976 are separated by 11 nucleotides
Pf6N2E2_1973 - FIG141751: hypothetical protein in PFGI-1-like cluster, at 2,339,249 to 2,340,397
_1973
Pf6N2E2_1974 - FIG141694: hypothetical protein in PFGI-1-like cluster, at 2,340,549 to 2,341,340
_1974
Pf6N2E2_1975 - Integrase regulator R, at 2,341,340 to 2,341,840
_1975
Pf6N2E2_1976 - Single-stranded DNA-binding protein, at 2,341,852 to 2,342,256
_1976
Position (kb)
2340
2341
2342 Strain fitness (log2 ratio)
-2
-1
0
1 at 2339.642 kb on + strand, within Pf6N2E2_1973 at 2339.642 kb on + strand, within Pf6N2E2_1973 at 2339.643 kb on - strand, within Pf6N2E2_1973 at 2339.643 kb on - strand, within Pf6N2E2_1973 at 2339.681 kb on - strand, within Pf6N2E2_1973 at 2339.700 kb on - strand, within Pf6N2E2_1973 at 2339.707 kb on - strand, within Pf6N2E2_1973 at 2339.707 kb on - strand, within Pf6N2E2_1973 at 2339.707 kb on - strand, within Pf6N2E2_1973 at 2339.707 kb on - strand, within Pf6N2E2_1973 at 2339.778 kb on + strand, within Pf6N2E2_1973 at 2339.779 kb on - strand, within Pf6N2E2_1973 at 2339.823 kb on + strand, within Pf6N2E2_1973 at 2339.823 kb on + strand, within Pf6N2E2_1973 at 2339.824 kb on - strand, within Pf6N2E2_1973 at 2339.824 kb on - strand, within Pf6N2E2_1973 at 2339.833 kb on - strand, within Pf6N2E2_1973 at 2339.878 kb on + strand, within Pf6N2E2_1973 at 2339.879 kb on - strand, within Pf6N2E2_1973 at 2339.879 kb on - strand, within Pf6N2E2_1973 at 2339.897 kb on - strand, within Pf6N2E2_1973 at 2339.998 kb on + strand, within Pf6N2E2_1973 at 2340.003 kb on + strand, within Pf6N2E2_1973 at 2340.003 kb on + strand, within Pf6N2E2_1973 at 2340.003 kb on + strand, within Pf6N2E2_1973 at 2340.004 kb on - strand, within Pf6N2E2_1973 at 2340.004 kb on - strand, within Pf6N2E2_1973 at 2340.004 kb on - strand, within Pf6N2E2_1973 at 2340.004 kb on - strand, within Pf6N2E2_1973 at 2340.007 kb on + strand, within Pf6N2E2_1973 at 2340.028 kb on - strand, within Pf6N2E2_1973 at 2340.031 kb on + strand, within Pf6N2E2_1973 at 2340.032 kb on - strand, within Pf6N2E2_1973 at 2340.032 kb on - strand, within Pf6N2E2_1973 at 2340.199 kb on - strand, within Pf6N2E2_1973 at 2340.199 kb on - strand, within Pf6N2E2_1973 at 2340.359 kb on + strand at 2340.359 kb on + strand at 2340.360 kb on - strand at 2340.738 kb on + strand, within Pf6N2E2_1974 at 2340.758 kb on - strand, within Pf6N2E2_1974 at 2340.890 kb on - strand, within Pf6N2E2_1974 at 2340.930 kb on + strand, within Pf6N2E2_1974 at 2340.931 kb on - strand, within Pf6N2E2_1974 at 2340.931 kb on - strand, within Pf6N2E2_1974 at 2340.945 kb on + strand, within Pf6N2E2_1974 at 2341.009 kb on + strand, within Pf6N2E2_1974 at 2341.072 kb on - strand, within Pf6N2E2_1974 at 2341.112 kb on + strand, within Pf6N2E2_1974 at 2341.112 kb on + strand, within Pf6N2E2_1974 at 2341.347 kb on + strand at 2341.347 kb on + strand at 2341.348 kb on - strand at 2341.407 kb on + strand, within Pf6N2E2_1975 at 2341.408 kb on - strand, within Pf6N2E2_1975 at 2341.727 kb on - strand, within Pf6N2E2_1975 at 2341.727 kb on - strand, within Pf6N2E2_1975 at 2341.805 kb on + strand at 2341.806 kb on - strand at 2341.836 kb on + strand at 2341.836 kb on + strand at 2341.837 kb on - strand at 2341.837 kb on - strand at 2341.879 kb on - strand at 2341.879 kb on - strand at 2341.882 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction perchlorate 0.00625 M remove 2,339,642 + Pf6N2E2_1973 0.34 +0.9 2,339,642 + Pf6N2E2_1973 0.34 -0.5 2,339,643 - Pf6N2E2_1973 0.34 +0.0 2,339,643 - Pf6N2E2_1973 0.34 -0.2 2,339,681 - Pf6N2E2_1973 0.38 +0.6 2,339,700 - Pf6N2E2_1973 0.39 +0.5 2,339,707 - Pf6N2E2_1973 0.40 -0.1 2,339,707 - Pf6N2E2_1973 0.40 +1.2 2,339,707 - Pf6N2E2_1973 0.40 +0.5 2,339,707 - Pf6N2E2_1973 0.40 -1.2 2,339,778 + Pf6N2E2_1973 0.46 -0.7 2,339,779 - Pf6N2E2_1973 0.46 -1.1 2,339,823 + Pf6N2E2_1973 0.50 -0.6 2,339,823 + Pf6N2E2_1973 0.50 -0.1 2,339,824 - Pf6N2E2_1973 0.50 -0.1 2,339,824 - Pf6N2E2_1973 0.50 -0.4 2,339,833 - Pf6N2E2_1973 0.51 -1.3 2,339,878 + Pf6N2E2_1973 0.55 -0.5 2,339,879 - Pf6N2E2_1973 0.55 +0.1 2,339,879 - Pf6N2E2_1973 0.55 -0.1 2,339,897 - Pf6N2E2_1973 0.56 -0.2 2,339,998 + Pf6N2E2_1973 0.65 +0.2 2,340,003 + Pf6N2E2_1973 0.66 -0.3 2,340,003 + Pf6N2E2_1973 0.66 +0.4 2,340,003 + Pf6N2E2_1973 0.66 -0.7 2,340,004 - Pf6N2E2_1973 0.66 +0.1 2,340,004 - Pf6N2E2_1973 0.66 +0.9 2,340,004 - Pf6N2E2_1973 0.66 +0.8 2,340,004 - Pf6N2E2_1973 0.66 +0.8 2,340,007 + Pf6N2E2_1973 0.66 +0.1 2,340,028 - Pf6N2E2_1973 0.68 -0.7 2,340,031 + Pf6N2E2_1973 0.68 +1.0 2,340,032 - Pf6N2E2_1973 0.68 -0.1 2,340,032 - Pf6N2E2_1973 0.68 +0.5 2,340,199 - Pf6N2E2_1973 0.83 +0.1 2,340,199 - Pf6N2E2_1973 0.83 -0.3 2,340,359 + -0.1 2,340,359 + -0.6 2,340,360 - +0.1 2,340,738 + Pf6N2E2_1974 0.24 -0.3 2,340,758 - Pf6N2E2_1974 0.26 -0.1 2,340,890 - Pf6N2E2_1974 0.43 -1.9 2,340,930 + Pf6N2E2_1974 0.48 -0.8 2,340,931 - Pf6N2E2_1974 0.48 -0.2 2,340,931 - Pf6N2E2_1974 0.48 -0.0 2,340,945 + Pf6N2E2_1974 0.50 -0.2 2,341,009 + Pf6N2E2_1974 0.58 -0.1 2,341,072 - Pf6N2E2_1974 0.66 +0.1 2,341,112 + Pf6N2E2_1974 0.71 -1.1 2,341,112 + Pf6N2E2_1974 0.71 -0.2 2,341,347 + +0.0 2,341,347 + +0.6 2,341,348 - +0.6 2,341,407 + Pf6N2E2_1975 0.13 -0.9 2,341,408 - Pf6N2E2_1975 0.14 +0.1 2,341,727 - Pf6N2E2_1975 0.77 +0.1 2,341,727 - Pf6N2E2_1975 0.77 +0.1 2,341,805 + +0.2 2,341,806 - +0.7 2,341,836 + +0.1 2,341,836 + -0.1 2,341,837 - -0.7 2,341,837 - -0.1 2,341,879 - +0.3 2,341,879 - +0.4 2,341,882 - -0.7
Or see this region's nucleotide sequence