Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5448

Experiment: DEANONOate 0.000625 M

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	DEANONOate 0.000625 M
6,254,354	+		Pf6N2E2_5447	0.25	+0.5
6,254,354	+		Pf6N2E2_5447	0.25	-3.0
6,254,354	+		Pf6N2E2_5447	0.25	-0.5
6,254,354	+		Pf6N2E2_5447	0.25	-3.3
6,254,354	+		Pf6N2E2_5447	0.25	-2.6
6,254,354	+		Pf6N2E2_5447	0.25	+0.8
6,254,355	-		Pf6N2E2_5447	0.25	-0.9
6,254,355	-		Pf6N2E2_5447	0.25	+1.1
6,254,355	-		Pf6N2E2_5447	0.25	-0.9
6,254,355	-		Pf6N2E2_5447	0.25	-2.0
6,254,489	+		Pf6N2E2_5447	0.49	-1.1
6,254,558	+		Pf6N2E2_5447	0.61	-1.4
6,254,558	+		Pf6N2E2_5447	0.61	-2.2
6,254,559	-		Pf6N2E2_5447	0.62	+0.4
6,254,559	-		Pf6N2E2_5447	0.62	+1.0
6,254,559	-		Pf6N2E2_5447	0.62	-1.4
6,254,583	-		Pf6N2E2_5447	0.66	-2.4
6,254,583	-		Pf6N2E2_5447	0.66	+0.2
6,254,598	+		Pf6N2E2_5447	0.68	+0.8
6,254,598	+		Pf6N2E2_5447	0.68	-1.8
6,254,599	-		Pf6N2E2_5447	0.69	-1.7
6,254,624	+		Pf6N2E2_5447	0.73	-0.2
6,254,624	+		Pf6N2E2_5447	0.73	-3.1
6,254,624	+		Pf6N2E2_5447	0.73	+1.8
6,254,625	-		Pf6N2E2_5447	0.73	+0.6
6,254,625	-		Pf6N2E2_5447	0.73	+0.4
6,254,762	+				+2.2
6,254,762	+				-0.6
6,254,763	-				+0.6
6,254,763	-				-0.2
6,254,775	+				-1.0
6,254,814	-				-0.8
6,254,815	+				+0.1
6,254,833	+				-3.4
6,254,918	+				+1.0
6,254,957	+				+1.2
6,255,048	+				+0.7
6,255,048	+				-0.4
6,255,048	+				-1.5
6,255,050	+				-0.8
6,255,062	-				-0.2
6,255,064	+				-0.5
6,255,064	+				-0.5
6,255,073	-				-2.2
6,255,090	+				-0.1
6,255,179	+				-0.0
6,255,180	-				-1.3
6,255,300	-		Pf6N2E2_5448	0.19	-1.0
6,255,300	-		Pf6N2E2_5448	0.19	-1.0
6,255,300	-		Pf6N2E2_5448	0.19	-3.4
6,255,318	-		Pf6N2E2_5448	0.23	-2.4
6,255,318	-		Pf6N2E2_5448	0.23	-1.3
6,255,334	+		Pf6N2E2_5448	0.26	-1.5
6,255,335	-		Pf6N2E2_5448	0.26	-0.2
6,255,335	-		Pf6N2E2_5448	0.26	-2.1
6,255,345	-		Pf6N2E2_5448	0.28	-2.7
6,255,378	+		Pf6N2E2_5448	0.35	-0.4
6,255,378	+		Pf6N2E2_5448	0.35	+0.5
6,255,378	+		Pf6N2E2_5448	0.35	-2.7
6,255,379	-		Pf6N2E2_5448	0.35	-2.8
6,255,420	+		Pf6N2E2_5448	0.44	-1.5
6,255,420	+		Pf6N2E2_5448	0.44	+0.2
6,255,420	+		Pf6N2E2_5448	0.44	+0.8
6,255,420	+		Pf6N2E2_5448	0.44	-0.7
6,255,421	-		Pf6N2E2_5448	0.44	+1.1
6,255,421	-		Pf6N2E2_5448	0.44	-2.7
6,255,421	-		Pf6N2E2_5448	0.44	-2.5
6,255,421	-		Pf6N2E2_5448	0.44	-1.9
6,255,456	+		Pf6N2E2_5448	0.51	+1.0
6,255,456	+		Pf6N2E2_5448	0.51	+1.8
6,255,457	-		Pf6N2E2_5448	0.51	-2.6
6,255,457	-		Pf6N2E2_5448	0.51	-2.9
6,255,457	-		Pf6N2E2_5448	0.51	-2.1
6,255,457	-		Pf6N2E2_5448	0.51	-3.0
6,255,457	-		Pf6N2E2_5448	0.51	-3.4
6,255,457	-		Pf6N2E2_5448	0.51	-0.9
6,255,457	-		Pf6N2E2_5448	0.51	-2.7
6,255,457	-		Pf6N2E2_5448	0.51	-3.6
6,255,457	-		Pf6N2E2_5448	0.51	+0.5
6,255,457	-		Pf6N2E2_5448	0.51	-3.7
6,255,623	+		Pf6N2E2_5448	0.85	+0.0
6,255,646	-		Pf6N2E2_5448	0.89	-2.4
6,255,811	+				-0.2
6,255,812	-				-1.0
6,255,863	+				-2.0
6,255,906	+				-0.8
6,255,907	-				-0.7
6,255,996	-				-1.5
6,256,012	+				-1.9
6,256,012	+				-2.7
6,256,012	+				-1.0
6,256,012	+				-0.9
6,256,013	-				+0.9
6,256,013	-				+0.4
6,256,013	-				-0.8
6,256,013	-				-1.9
6,256,056	+				-0.0
6,256,056	+				+0.2
6,256,057	-				-2.5
6,256,057	-				+0.5
6,256,057	-				-0.1
6,256,057	-				-1.0
6,256,195	+		Pf6N2E2_5449	0.15	-1.9
6,256,195	+		Pf6N2E2_5449	0.15	+0.0
6,256,440	+		Pf6N2E2_5449	0.24	-0.4
6,256,441	-		Pf6N2E2_5449	0.24	-3.3
6,256,447	+		Pf6N2E2_5449	0.25	-1.8
6,256,448	-		Pf6N2E2_5449	0.25	+0.4
6,256,511	-		Pf6N2E2_5449	0.27	-2.0
6,256,591	+		Pf6N2E2_5449	0.30	+1.1
6,256,591	+		Pf6N2E2_5449	0.30	+2.2
6,256,591	+		Pf6N2E2_5449	0.30	-1.9
6,256,592	-		Pf6N2E2_5449	0.30	+0.4
6,256,592	-		Pf6N2E2_5449	0.30	+1.6
6,256,592	-		Pf6N2E2_5449	0.30	+0.4
6,256,592	-		Pf6N2E2_5449	0.30	-2.6
6,256,649	-		Pf6N2E2_5449	0.33	+1.8
6,256,649	-		Pf6N2E2_5449	0.33	+2.0
6,256,682	+		Pf6N2E2_5449	0.34	+1.5
6,256,682	+		Pf6N2E2_5449	0.34	+0.6
6,256,682	+		Pf6N2E2_5449	0.34	+1.2
6,256,682	+		Pf6N2E2_5449	0.34	+0.5
6,256,683	-		Pf6N2E2_5449	0.34	+1.0
6,256,683	-		Pf6N2E2_5449	0.34	-0.2
6,256,683	-		Pf6N2E2_5449	0.34	+0.5
6,256,684	+		Pf6N2E2_5449	0.34	-0.7
6,256,684	+		Pf6N2E2_5449	0.34	-0.5
6,256,684	+		Pf6N2E2_5449	0.34	+1.2
6,256,684	+		Pf6N2E2_5449	0.34	+0.7
6,256,684	+		Pf6N2E2_5449	0.34	+1.0
6,256,684	+		Pf6N2E2_5449	0.34	+0.4
6,256,684	+		Pf6N2E2_5449	0.34	+0.2
6,256,685	-		Pf6N2E2_5449	0.34	-0.9