Experiment: DEANONOate 0.000625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3739 and Pf6N2E2_3740 overlap by 4 nucleotides Pf6N2E2_3740 and Pf6N2E2_3741 overlap by 8 nucleotides Pf6N2E2_3741 and Pf6N2E2_3742 overlap by 8 nucleotides Pf6N2E2_3742 and Pf6N2E2_3743 are separated by 2 nucleotides Pf6N2E2_3743 and Pf6N2E2_3744 are separated by 3 nucleotides
Pf6N2E2_3739 - General secretion pathway protein K, at 4,355,477 to 4,356,325
_3739
Pf6N2E2_3740 - General secretion pathway protein J, at 4,356,322 to 4,356,855
_3740
Pf6N2E2_3741 - General secretion pathway protein I, at 4,356,848 to 4,357,234
_3741
Pf6N2E2_3742 - General secretion pathway protein H, at 4,357,227 to 4,357,694
_3742
Pf6N2E2_3743 - General secretion pathway protein G, at 4,357,697 to 4,358,152
_3743
Pf6N2E2_3744 - General secretion pathway protein F, at 4,358,156 to 4,359,364
_3744
Position (kb)
4357
4358 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4356.455 kb on - strand, within Pf6N2E2_3740 at 4356.455 kb on - strand, within Pf6N2E2_3740 at 4356.455 kb on - strand, within Pf6N2E2_3740 at 4356.475 kb on + strand, within Pf6N2E2_3740 at 4356.475 kb on + strand, within Pf6N2E2_3740 at 4356.475 kb on + strand, within Pf6N2E2_3740 at 4356.476 kb on - strand, within Pf6N2E2_3740 at 4356.476 kb on - strand, within Pf6N2E2_3740 at 4356.615 kb on + strand, within Pf6N2E2_3740 at 4356.616 kb on - strand, within Pf6N2E2_3740 at 4356.849 kb on + strand at 4356.849 kb on + strand at 4356.849 kb on + strand at 4356.850 kb on - strand at 4357.491 kb on + strand, within Pf6N2E2_3742 at 4357.491 kb on + strand, within Pf6N2E2_3742 at 4357.491 kb on + strand, within Pf6N2E2_3742 at 4357.491 kb on + strand, within Pf6N2E2_3742 at 4357.497 kb on + strand, within Pf6N2E2_3742 at 4357.498 kb on - strand, within Pf6N2E2_3742 at 4357.498 kb on - strand, within Pf6N2E2_3742 at 4357.504 kb on + strand, within Pf6N2E2_3742 at 4357.767 kb on + strand, within Pf6N2E2_3743 at 4357.768 kb on - strand, within Pf6N2E2_3743 at 4357.768 kb on - strand, within Pf6N2E2_3743 at 4357.818 kb on + strand, within Pf6N2E2_3743 at 4357.857 kb on + strand, within Pf6N2E2_3743 at 4357.858 kb on - strand, within Pf6N2E2_3743 at 4357.942 kb on - strand, within Pf6N2E2_3743 at 4357.963 kb on - strand, within Pf6N2E2_3743 at 4358.038 kb on + strand, within Pf6N2E2_3743 at 4358.039 kb on - strand, within Pf6N2E2_3743 at 4358.039 kb on - strand, within Pf6N2E2_3743 at 4358.075 kb on - strand, within Pf6N2E2_3743 at 4358.185 kb on + strand at 4358.185 kb on + strand at 4358.186 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction DEANONOate 0.000625 M remove 4,356,455 - Pf6N2E2_3740 0.25 +1.5 4,356,455 - Pf6N2E2_3740 0.25 +0.3 4,356,455 - Pf6N2E2_3740 0.25 +0.4 4,356,475 + Pf6N2E2_3740 0.29 -0.9 4,356,475 + Pf6N2E2_3740 0.29 +1.4 4,356,475 + Pf6N2E2_3740 0.29 -3.2 4,356,476 - Pf6N2E2_3740 0.29 +0.3 4,356,476 - Pf6N2E2_3740 0.29 +1.4 4,356,615 + Pf6N2E2_3740 0.55 +0.6 4,356,616 - Pf6N2E2_3740 0.55 +1.4 4,356,849 + -1.6 4,356,849 + -0.9 4,356,849 + -1.9 4,356,850 - +0.8 4,357,491 + Pf6N2E2_3742 0.56 -2.7 4,357,491 + Pf6N2E2_3742 0.56 -1.9 4,357,491 + Pf6N2E2_3742 0.56 -2.6 4,357,491 + Pf6N2E2_3742 0.56 -0.4 4,357,497 + Pf6N2E2_3742 0.58 -0.5 4,357,498 - Pf6N2E2_3742 0.58 +0.2 4,357,498 - Pf6N2E2_3742 0.58 -2.1 4,357,504 + Pf6N2E2_3742 0.59 -0.7 4,357,767 + Pf6N2E2_3743 0.15 +0.4 4,357,768 - Pf6N2E2_3743 0.16 -0.2 4,357,768 - Pf6N2E2_3743 0.16 +0.3 4,357,818 + Pf6N2E2_3743 0.27 -0.4 4,357,857 + Pf6N2E2_3743 0.35 -0.6 4,357,858 - Pf6N2E2_3743 0.35 -0.6 4,357,942 - Pf6N2E2_3743 0.54 -0.4 4,357,963 - Pf6N2E2_3743 0.58 -2.4 4,358,038 + Pf6N2E2_3743 0.75 -1.3 4,358,039 - Pf6N2E2_3743 0.75 +0.5 4,358,039 - Pf6N2E2_3743 0.75 +0.0 4,358,075 - Pf6N2E2_3743 0.83 +1.8 4,358,185 + -0.1 4,358,185 + +0.8 4,358,186 - -3.2
Or see this region's nucleotide sequence