Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_278

Experiment: DEANONOate 0.000625 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_277 and Pf6N2E2_278 are separated by 871 nucleotidesPf6N2E2_278 and Pf6N2E2_279 are separated by 107 nucleotides Pf6N2E2_277 - NADH ubiquinone oxidoreductase chain A (EC 1.6.5.3), at 342,559 to 342,972 _277 Pf6N2E2_278 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 343,844 to 345,766 _278 Pf6N2E2_279 - Isocitrate lyase (EC 4.1.3.1), at 345,874 to 347,199 _279 Position (kb) 343 344 345 346Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 343.016 kb on - strandat 343.036 kb on - strandat 343.106 kb on - strandat 343.118 kb on - strandat 343.125 kb on + strandat 343.126 kb on - strandat 343.189 kb on - strandat 343.189 kb on - strandat 343.269 kb on + strandat 343.269 kb on + strandat 343.269 kb on + strandat 343.269 kb on + strandat 343.270 kb on - strandat 343.270 kb on - strandat 343.294 kb on + strandat 343.294 kb on + strandat 343.294 kb on + strandat 343.294 kb on + strandat 343.332 kb on + strandat 343.354 kb on + strandat 343.549 kb on + strandat 343.550 kb on - strandat 343.556 kb on + strandat 343.556 kb on + strandat 343.556 kb on + strandat 343.556 kb on + strandat 343.556 kb on + strandat 343.557 kb on - strandat 343.557 kb on - strandat 343.634 kb on + strandat 343.634 kb on + strandat 343.635 kb on - strandat 343.701 kb on - strandat 343.701 kb on - strandat 343.752 kb on - strandat 343.752 kb on - strandat 343.759 kb on + strandat 343.759 kb on + strandat 343.775 kb on - strandat 343.792 kb on + strandat 343.792 kb on + strandat 343.792 kb on + strandat 343.792 kb on + strandat 343.792 kb on + strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.796 kb on + strandat 343.796 kb on + strandat 343.797 kb on - strandat 343.797 kb on - strandat 343.797 kb on - strandat 343.797 kb on - strandat 343.797 kb on - strandat 343.829 kb on + strandat 343.830 kb on - strandat 343.991 kb on - strandat 344.039 kb on + strand, within Pf6N2E2_278at 344.206 kb on + strand, within Pf6N2E2_278at 344.206 kb on + strand, within Pf6N2E2_278at 344.307 kb on + strand, within Pf6N2E2_278at 344.308 kb on - strand, within Pf6N2E2_278at 344.308 kb on - strand, within Pf6N2E2_278at 344.349 kb on - strand, within Pf6N2E2_278at 344.393 kb on + strand, within Pf6N2E2_278at 344.510 kb on + strand, within Pf6N2E2_278at 344.511 kb on - strand, within Pf6N2E2_278at 344.585 kb on - strand, within Pf6N2E2_278at 344.585 kb on - strand, within Pf6N2E2_278at 344.660 kb on + strand, within Pf6N2E2_278at 344.848 kb on + strand, within Pf6N2E2_278at 344.848 kb on + strand, within Pf6N2E2_278at 344.893 kb on + strand, within Pf6N2E2_278at 344.894 kb on - strand, within Pf6N2E2_278at 345.469 kb on + strand, within Pf6N2E2_278at 345.661 kb on - strandat 345.661 kb on - strandat 346.001 kb on + strandat 346.002 kb on - strandat 346.071 kb on - strand, within Pf6N2E2_279at 346.142 kb on + strand, within Pf6N2E2_279at 346.143 kb on - strand, within Pf6N2E2_279at 346.229 kb on + strand, within Pf6N2E2_279at 346.229 kb on + strand, within Pf6N2E2_279at 346.229 kb on + strand, within Pf6N2E2_279at 346.230 kb on - strand, within Pf6N2E2_279at 346.388 kb on + strand, within Pf6N2E2_279at 346.388 kb on + strand, within Pf6N2E2_279at 346.388 kb on + strand, within Pf6N2E2_279at 346.388 kb on + strand, within Pf6N2E2_279at 346.388 kb on + strand, within Pf6N2E2_279at 346.388 kb on + strand, within Pf6N2E2_279at 346.389 kb on - strand, within Pf6N2E2_279at 346.389 kb on - strand, within Pf6N2E2_279at 346.389 kb on - strand, within Pf6N2E2_279at 346.480 kb on - strand, within Pf6N2E2_279at 346.670 kb on + strand, within Pf6N2E2_279at 346.728 kb on - strand, within Pf6N2E2_279at 346.736 kb on + strand, within Pf6N2E2_279at 346.737 kb on - strand, within Pf6N2E2_279at 346.737 kb on - strand, within Pf6N2E2_279at 346.737 kb on - strand, within Pf6N2E2_279

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Per-strain Table

Position Strand Gene LocusTag Fraction DEANONOate 0.000625 M
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343,016 - -2.7
343,036 - -2.4
343,106 - -0.4
343,118 - -0.3
343,125 + -1.5
343,126 - -0.6
343,189 - -1.0
343,189 - +0.6
343,269 + -3.4
343,269 + -3.8
343,269 + -2.3
343,269 + -2.0
343,270 - +1.2
343,270 - -0.9
343,294 + -2.6
343,294 + -3.6
343,294 + -2.0
343,294 + -1.1
343,332 + -2.9
343,354 + -3.1
343,549 + -0.8
343,550 - -0.5
343,556 + -1.4
343,556 + -1.0
343,556 + -2.5
343,556 + -2.1
343,556 + -2.5
343,557 - +1.0
343,557 - +0.7
343,634 + -1.0
343,634 + -2.4
343,635 - +1.2
343,701 - +2.3
343,701 - +0.6
343,752 - -0.9
343,752 - +1.4
343,759 + -2.7
343,759 + -2.3
343,775 - +1.1
343,792 + -3.9
343,792 + -0.5
343,792 + +1.0
343,792 + -0.1
343,792 + -1.8
343,793 - +0.8
343,793 - +1.1
343,793 - -0.6
343,793 - +2.3
343,793 - -0.0
343,793 - +0.5
343,793 - -0.0
343,793 - +1.5
343,793 - -2.1
343,793 - -2.4
343,793 - -1.5
343,796 + -3.2
343,796 + -0.3
343,797 - -0.0
343,797 - -0.9
343,797 - -1.0
343,797 - +1.4
343,797 - +2.0
343,829 + -0.7
343,830 - -1.6
343,991 - -1.2
344,039 + Pf6N2E2_278 0.10 -1.5
344,206 + Pf6N2E2_278 0.19 -1.5
344,206 + Pf6N2E2_278 0.19 -0.6
344,307 + Pf6N2E2_278 0.24 -2.5
344,308 - Pf6N2E2_278 0.24 -1.0
344,308 - Pf6N2E2_278 0.24 +0.5
344,349 - Pf6N2E2_278 0.26 -0.5
344,393 + Pf6N2E2_278 0.29 +0.0
344,510 + Pf6N2E2_278 0.35 -1.2
344,511 - Pf6N2E2_278 0.35 +1.0
344,585 - Pf6N2E2_278 0.39 +1.1
344,585 - Pf6N2E2_278 0.39 -0.8
344,660 + Pf6N2E2_278 0.42 +0.3
344,848 + Pf6N2E2_278 0.52 -2.9
344,848 + Pf6N2E2_278 0.52 -0.4
344,893 + Pf6N2E2_278 0.55 -3.0
344,894 - Pf6N2E2_278 0.55 +1.5
345,469 + Pf6N2E2_278 0.85 -1.9
345,661 - -1.5
345,661 - +0.7
346,001 + +0.7
346,002 - -0.9
346,071 - Pf6N2E2_279 0.15 +0.6
346,142 + Pf6N2E2_279 0.20 -0.0
346,143 - Pf6N2E2_279 0.20 +1.1
346,229 + Pf6N2E2_279 0.27 -0.9
346,229 + Pf6N2E2_279 0.27 -0.4
346,229 + Pf6N2E2_279 0.27 -3.0
346,230 - Pf6N2E2_279 0.27 -0.9
346,388 + Pf6N2E2_279 0.39 -1.1
346,388 + Pf6N2E2_279 0.39 +0.1
346,388 + Pf6N2E2_279 0.39 +0.5
346,388 + Pf6N2E2_279 0.39 -0.4
346,388 + Pf6N2E2_279 0.39 -1.0
346,388 + Pf6N2E2_279 0.39 -2.4
346,389 - Pf6N2E2_279 0.39 -0.3
346,389 - Pf6N2E2_279 0.39 -0.9
346,389 - Pf6N2E2_279 0.39 +0.8
346,480 - Pf6N2E2_279 0.46 -1.0
346,670 + Pf6N2E2_279 0.60 -0.4
346,728 - Pf6N2E2_279 0.64 -0.9
346,736 + Pf6N2E2_279 0.65 +0.3
346,737 - Pf6N2E2_279 0.65 +0.8
346,737 - Pf6N2E2_279 0.65 -0.6
346,737 - Pf6N2E2_279 0.65 -1.0

Or see this region's nucleotide sequence