Experiment: DEANONOate 0.000625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1226 and Pf6N2E2_1227 are separated by 220 nucleotides Pf6N2E2_1227 and Pf6N2E2_1228 are separated by 128 nucleotides Pf6N2E2_1228 and Pf6N2E2_1229 are separated by 169 nucleotides
Pf6N2E2_1226 - DNA damage response nuclease (from data) , at 1,434,468 to 1,436,138
_1226
Pf6N2E2_1227 - FIG00955097: hypothetical protein, at 1,436,359 to 1,436,583
_1227
Pf6N2E2_1228 - Lipase (EC 3.1.1.3), at 1,436,712 to 1,438,565
_1228
Pf6N2E2_1229 - Serine protease homologue, at 1,438,735 to 1,441,827
_1229
Position (kb)
1436
1437
1438
1439 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1436.104 kb on + strand at 1436.104 kb on + strand at 1436.107 kb on + strand at 1436.107 kb on + strand at 1436.108 kb on - strand at 1436.108 kb on - strand at 1436.191 kb on + strand at 1436.229 kb on + strand at 1436.231 kb on + strand at 1436.231 kb on + strand at 1436.231 kb on + strand at 1436.232 kb on - strand at 1436.232 kb on - strand at 1436.232 kb on - strand at 1436.235 kb on + strand at 1436.235 kb on + strand at 1436.236 kb on - strand at 1436.236 kb on - strand at 1436.236 kb on - strand at 1436.352 kb on + strand at 1436.461 kb on + strand, within Pf6N2E2_1227 at 1436.652 kb on + strand at 1436.652 kb on + strand at 1436.653 kb on - strand at 1436.658 kb on + strand at 1436.659 kb on - strand at 1436.665 kb on + strand at 1436.665 kb on + strand at 1436.666 kb on - strand at 1436.821 kb on + strand at 1436.821 kb on + strand at 1436.822 kb on - strand at 1436.830 kb on + strand at 1437.106 kb on + strand, within Pf6N2E2_1228 at 1437.106 kb on + strand, within Pf6N2E2_1228 at 1437.106 kb on + strand, within Pf6N2E2_1228 at 1437.107 kb on - strand, within Pf6N2E2_1228 at 1437.107 kb on - strand, within Pf6N2E2_1228 at 1437.107 kb on - strand, within Pf6N2E2_1228 at 1437.259 kb on + strand, within Pf6N2E2_1228 at 1437.259 kb on + strand, within Pf6N2E2_1228 at 1437.260 kb on - strand, within Pf6N2E2_1228 at 1437.260 kb on - strand, within Pf6N2E2_1228 at 1437.323 kb on + strand, within Pf6N2E2_1228 at 1437.323 kb on + strand, within Pf6N2E2_1228 at 1437.323 kb on + strand, within Pf6N2E2_1228 at 1437.324 kb on - strand, within Pf6N2E2_1228 at 1437.324 kb on - strand, within Pf6N2E2_1228 at 1437.324 kb on - strand, within Pf6N2E2_1228 at 1437.324 kb on - strand, within Pf6N2E2_1228 at 1437.324 kb on - strand, within Pf6N2E2_1228 at 1437.326 kb on + strand, within Pf6N2E2_1228 at 1437.326 kb on + strand, within Pf6N2E2_1228 at 1437.327 kb on - strand, within Pf6N2E2_1228 at 1437.353 kb on + strand, within Pf6N2E2_1228 at 1437.354 kb on - strand, within Pf6N2E2_1228 at 1437.354 kb on - strand, within Pf6N2E2_1228 at 1437.355 kb on + strand, within Pf6N2E2_1228 at 1437.355 kb on + strand, within Pf6N2E2_1228 at 1437.509 kb on + strand, within Pf6N2E2_1228 at 1437.509 kb on + strand, within Pf6N2E2_1228 at 1437.510 kb on - strand, within Pf6N2E2_1228 at 1437.575 kb on - strand, within Pf6N2E2_1228 at 1437.691 kb on + strand, within Pf6N2E2_1228 at 1437.691 kb on + strand, within Pf6N2E2_1228 at 1437.868 kb on + strand, within Pf6N2E2_1228 at 1437.883 kb on + strand, within Pf6N2E2_1228 at 1438.055 kb on - strand, within Pf6N2E2_1228 at 1438.055 kb on - strand, within Pf6N2E2_1228 at 1438.186 kb on + strand, within Pf6N2E2_1228 at 1438.204 kb on - strand, within Pf6N2E2_1228 at 1438.220 kb on - strand, within Pf6N2E2_1228 at 1438.220 kb on - strand, within Pf6N2E2_1228 at 1438.480 kb on + strand at 1438.481 kb on - strand at 1438.481 kb on - strand at 1438.481 kb on - strand at 1438.486 kb on + strand at 1438.486 kb on + strand at 1438.486 kb on + strand at 1438.487 kb on - strand at 1438.549 kb on + strand at 1438.549 kb on + strand at 1438.549 kb on + strand at 1438.550 kb on - strand at 1438.550 kb on - strand at 1438.555 kb on + strand at 1438.555 kb on + strand at 1438.555 kb on + strand at 1438.555 kb on + strand at 1438.559 kb on + strand at 1438.559 kb on + strand at 1438.559 kb on + strand at 1438.560 kb on - strand at 1438.596 kb on + strand at 1438.597 kb on - strand at 1438.597 kb on - strand at 1438.609 kb on + strand at 1438.610 kb on - strand at 1438.652 kb on + strand at 1438.652 kb on + strand at 1438.652 kb on + strand at 1438.802 kb on + strand at 1438.803 kb on - strand at 1438.803 kb on - strand at 1438.987 kb on + strand at 1438.987 kb on + strand at 1438.987 kb on + strand at 1438.988 kb on - strand at 1438.988 kb on - strand at 1438.988 kb on - strand at 1438.988 kb on - strand at 1438.988 kb on - strand at 1438.988 kb on - strand at 1439.016 kb on + strand at 1439.017 kb on - strand at 1439.129 kb on + strand, within Pf6N2E2_1229 at 1439.129 kb on + strand, within Pf6N2E2_1229 at 1439.129 kb on + strand, within Pf6N2E2_1229 at 1439.130 kb on - strand, within Pf6N2E2_1229 at 1439.130 kb on - strand, within Pf6N2E2_1229 at 1439.130 kb on - strand, within Pf6N2E2_1229 at 1439.130 kb on - strand, within Pf6N2E2_1229 at 1439.130 kb on - strand, within Pf6N2E2_1229 at 1439.354 kb on + strand, within Pf6N2E2_1229 at 1439.355 kb on - strand, within Pf6N2E2_1229 at 1439.414 kb on + strand, within Pf6N2E2_1229 at 1439.497 kb on - strand, within Pf6N2E2_1229
Per-strain Table
Position Strand Gene LocusTag Fraction DEANONOate 0.000625 M remove 1,436,104 + +0.4 1,436,104 + +0.0 1,436,107 + +2.6 1,436,107 + +0.9 1,436,108 - -0.8 1,436,108 - +0.1 1,436,191 + -0.8 1,436,229 + +1.0 1,436,231 + +0.3 1,436,231 + -1.5 1,436,231 + +0.3 1,436,232 - -0.5 1,436,232 - -1.2 1,436,232 - -1.1 1,436,235 + -1.2 1,436,235 + -0.6 1,436,236 - -0.8 1,436,236 - +0.4 1,436,236 - +0.0 1,436,352 + -0.5 1,436,461 + Pf6N2E2_1227 0.45 +1.2 1,436,652 + -0.1 1,436,652 + +2.0 1,436,653 - -0.4 1,436,658 + +0.2 1,436,659 - -1.7 1,436,665 + +1.6 1,436,665 + -0.2 1,436,666 - +1.9 1,436,821 + +1.7 1,436,821 + +1.9 1,436,822 - +1.1 1,436,830 + +1.1 1,437,106 + Pf6N2E2_1228 0.21 -0.3 1,437,106 + Pf6N2E2_1228 0.21 +0.5 1,437,106 + Pf6N2E2_1228 0.21 +0.6 1,437,107 - Pf6N2E2_1228 0.21 -3.5 1,437,107 - Pf6N2E2_1228 0.21 +1.3 1,437,107 - Pf6N2E2_1228 0.21 -1.4 1,437,259 + Pf6N2E2_1228 0.30 +0.1 1,437,259 + Pf6N2E2_1228 0.30 -0.8 1,437,260 - Pf6N2E2_1228 0.30 -2.2 1,437,260 - Pf6N2E2_1228 0.30 +1.4 1,437,323 + Pf6N2E2_1228 0.33 +0.4 1,437,323 + Pf6N2E2_1228 0.33 -0.2 1,437,323 + Pf6N2E2_1228 0.33 +0.5 1,437,324 - Pf6N2E2_1228 0.33 -2.4 1,437,324 - Pf6N2E2_1228 0.33 -1.9 1,437,324 - Pf6N2E2_1228 0.33 +0.6 1,437,324 - Pf6N2E2_1228 0.33 -0.8 1,437,324 - Pf6N2E2_1228 0.33 -0.1 1,437,326 + Pf6N2E2_1228 0.33 +0.7 1,437,326 + Pf6N2E2_1228 0.33 -1.8 1,437,327 - Pf6N2E2_1228 0.33 +0.0 1,437,353 + Pf6N2E2_1228 0.35 +1.1 1,437,354 - Pf6N2E2_1228 0.35 -1.0 1,437,354 - Pf6N2E2_1228 0.35 -0.5 1,437,355 + Pf6N2E2_1228 0.35 +0.5 1,437,355 + Pf6N2E2_1228 0.35 -2.4 1,437,509 + Pf6N2E2_1228 0.43 -1.0 1,437,509 + Pf6N2E2_1228 0.43 +0.9 1,437,510 - Pf6N2E2_1228 0.43 +0.5 1,437,575 - Pf6N2E2_1228 0.47 -0.2 1,437,691 + Pf6N2E2_1228 0.53 +0.2 1,437,691 + Pf6N2E2_1228 0.53 -2.9 1,437,868 + Pf6N2E2_1228 0.62 +0.6 1,437,883 + Pf6N2E2_1228 0.63 +0.5 1,438,055 - Pf6N2E2_1228 0.72 -0.4 1,438,055 - Pf6N2E2_1228 0.72 -1.9 1,438,186 + Pf6N2E2_1228 0.80 -1.8 1,438,204 - Pf6N2E2_1228 0.80 +0.2 1,438,220 - Pf6N2E2_1228 0.81 -1.4 1,438,220 - Pf6N2E2_1228 0.81 +0.4 1,438,480 + -2.8 1,438,481 - +3.4 1,438,481 - +0.1 1,438,481 - -0.2 1,438,486 + -0.9 1,438,486 + +2.3 1,438,486 + +0.7 1,438,487 - +0.9 1,438,549 + -1.9 1,438,549 + +0.3 1,438,549 + -1.4 1,438,550 - -2.6 1,438,550 - +1.3 1,438,555 + +0.6 1,438,555 + +1.3 1,438,555 + +1.1 1,438,555 + +3.4 1,438,559 + -1.9 1,438,559 + +0.8 1,438,559 + -2.4 1,438,560 - -2.3 1,438,596 + -2.3 1,438,597 - -0.2 1,438,597 - -0.4 1,438,609 + -1.0 1,438,610 - -1.4 1,438,652 + -0.9 1,438,652 + -0.5 1,438,652 + +0.1 1,438,802 + +0.8 1,438,803 - -1.1 1,438,803 - +0.4 1,438,987 + +0.4 1,438,987 + -0.2 1,438,987 + +0.7 1,438,988 - -2.4 1,438,988 - -0.4 1,438,988 - -0.5 1,438,988 - -0.9 1,438,988 - -3.6 1,438,988 - +1.0 1,439,016 + -0.9 1,439,017 - -1.1 1,439,129 + Pf6N2E2_1229 0.13 +3.1 1,439,129 + Pf6N2E2_1229 0.13 +0.3 1,439,129 + Pf6N2E2_1229 0.13 -2.6 1,439,130 - Pf6N2E2_1229 0.13 +1.8 1,439,130 - Pf6N2E2_1229 0.13 -1.1 1,439,130 - Pf6N2E2_1229 0.13 -0.8 1,439,130 - Pf6N2E2_1229 0.13 -1.5 1,439,130 - Pf6N2E2_1229 0.13 +0.1 1,439,354 + Pf6N2E2_1229 0.20 -1.4 1,439,355 - Pf6N2E2_1229 0.20 +0.8 1,439,414 + Pf6N2E2_1229 0.22 -2.7 1,439,497 - Pf6N2E2_1229 0.25 +0.2
Or see this region's nucleotide sequence