Experiment: DEANONOate 0.000625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1050 and Pf6N2E2_1051 are separated by 221 nucleotides Pf6N2E2_1051 and Pf6N2E2_1052 are separated by 741 nucleotides
Pf6N2E2_1050 - Enoyl-[acyl-carrier-protein] reductase [NADPH] (EC 1.3.1.10), at 1,223,916 to 1,224,467
_1050
Pf6N2E2_1051 - Probable NADH-dependent flavin oxidoreductase yqiG (EC 1.-.-.-), at 1,224,689 to 1,226,044
_1051
Pf6N2E2_1052 - hypothetical protein, at 1,226,786 to 1,226,926
_1052
Position (kb)
1224
1225
1226
1227 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1223.803 kb on + strand at 1223.804 kb on - strand at 1223.836 kb on + strand at 1223.836 kb on + strand at 1223.836 kb on + strand at 1223.837 kb on - strand at 1223.951 kb on + strand at 1223.951 kb on + strand at 1223.951 kb on + strand at 1223.952 kb on - strand at 1223.952 kb on - strand at 1223.952 kb on - strand at 1223.952 kb on - strand at 1223.952 kb on - strand at 1223.961 kb on + strand at 1223.962 kb on - strand at 1223.969 kb on - strand at 1224.067 kb on + strand, within Pf6N2E2_1050 at 1224.068 kb on - strand, within Pf6N2E2_1050 at 1224.068 kb on - strand, within Pf6N2E2_1050 at 1224.116 kb on + strand, within Pf6N2E2_1050 at 1224.156 kb on + strand, within Pf6N2E2_1050 at 1224.156 kb on + strand, within Pf6N2E2_1050 at 1224.156 kb on + strand, within Pf6N2E2_1050 at 1224.157 kb on - strand, within Pf6N2E2_1050 at 1224.188 kb on + strand, within Pf6N2E2_1050 at 1224.188 kb on + strand, within Pf6N2E2_1050 at 1224.189 kb on - strand, within Pf6N2E2_1050 at 1224.196 kb on + strand, within Pf6N2E2_1050 at 1224.196 kb on + strand, within Pf6N2E2_1050 at 1224.338 kb on + strand, within Pf6N2E2_1050 at 1224.338 kb on + strand, within Pf6N2E2_1050 at 1224.338 kb on + strand, within Pf6N2E2_1050 at 1224.339 kb on - strand, within Pf6N2E2_1050 at 1224.339 kb on - strand, within Pf6N2E2_1050 at 1224.339 kb on - strand, within Pf6N2E2_1050 at 1224.339 kb on - strand, within Pf6N2E2_1050 at 1224.339 kb on - strand, within Pf6N2E2_1050 at 1224.339 kb on - strand, within Pf6N2E2_1050 at 1224.339 kb on - strand, within Pf6N2E2_1050 at 1224.350 kb on + strand, within Pf6N2E2_1050 at 1224.351 kb on - strand, within Pf6N2E2_1050 at 1224.351 kb on - strand, within Pf6N2E2_1050 at 1224.453 kb on + strand at 1224.455 kb on + strand at 1224.456 kb on - strand at 1224.456 kb on - strand at 1224.456 kb on - strand at 1224.468 kb on + strand at 1224.487 kb on + strand at 1224.531 kb on - strand at 1224.531 kb on - strand at 1224.531 kb on - strand at 1224.532 kb on + strand at 1224.542 kb on + strand at 1224.543 kb on - strand at 1224.543 kb on - strand at 1224.543 kb on - strand at 1224.551 kb on + strand at 1224.596 kb on - strand at 1224.686 kb on - strand at 1224.701 kb on - strand at 1224.701 kb on - strand at 1224.701 kb on - strand at 1224.777 kb on + strand at 1224.777 kb on + strand at 1224.778 kb on - strand at 1224.778 kb on - strand at 1224.817 kb on + strand at 1224.817 kb on + strand at 1224.832 kb on + strand, within Pf6N2E2_1051 at 1224.832 kb on + strand, within Pf6N2E2_1051 at 1224.832 kb on + strand, within Pf6N2E2_1051 at 1224.832 kb on + strand, within Pf6N2E2_1051 at 1224.833 kb on - strand, within Pf6N2E2_1051 at 1224.833 kb on - strand, within Pf6N2E2_1051 at 1224.833 kb on - strand, within Pf6N2E2_1051 at 1224.833 kb on - strand, within Pf6N2E2_1051 at 1224.841 kb on + strand, within Pf6N2E2_1051 at 1224.841 kb on + strand, within Pf6N2E2_1051 at 1224.841 kb on + strand, within Pf6N2E2_1051 at 1224.841 kb on + strand, within Pf6N2E2_1051 at 1224.842 kb on - strand, within Pf6N2E2_1051 at 1224.843 kb on + strand, within Pf6N2E2_1051 at 1224.844 kb on - strand, within Pf6N2E2_1051 at 1224.844 kb on - strand, within Pf6N2E2_1051 at 1224.844 kb on - strand at 1224.844 kb on - strand, within Pf6N2E2_1051 at 1224.844 kb on - strand, within Pf6N2E2_1051 at 1224.849 kb on + strand, within Pf6N2E2_1051 at 1224.849 kb on + strand, within Pf6N2E2_1051 at 1224.850 kb on - strand, within Pf6N2E2_1051 at 1224.850 kb on - strand, within Pf6N2E2_1051 at 1224.850 kb on - strand, within Pf6N2E2_1051 at 1224.850 kb on - strand, within Pf6N2E2_1051 at 1224.919 kb on - strand, within Pf6N2E2_1051 at 1224.919 kb on - strand, within Pf6N2E2_1051 at 1224.919 kb on - strand, within Pf6N2E2_1051 at 1224.919 kb on - strand, within Pf6N2E2_1051 at 1224.953 kb on + strand, within Pf6N2E2_1051 at 1224.954 kb on - strand, within Pf6N2E2_1051 at 1225.133 kb on + strand, within Pf6N2E2_1051 at 1225.133 kb on + strand, within Pf6N2E2_1051 at 1225.134 kb on - strand, within Pf6N2E2_1051 at 1225.137 kb on + strand, within Pf6N2E2_1051 at 1225.137 kb on + strand at 1225.137 kb on + strand, within Pf6N2E2_1051 at 1225.137 kb on + strand, within Pf6N2E2_1051 at 1225.137 kb on + strand, within Pf6N2E2_1051 at 1225.137 kb on + strand, within Pf6N2E2_1051 at 1225.137 kb on + strand, within Pf6N2E2_1051 at 1225.138 kb on - strand, within Pf6N2E2_1051 at 1225.381 kb on - strand, within Pf6N2E2_1051 at 1225.381 kb on - strand, within Pf6N2E2_1051 at 1225.381 kb on - strand, within Pf6N2E2_1051 at 1225.381 kb on - strand, within Pf6N2E2_1051 at 1225.432 kb on - strand, within Pf6N2E2_1051 at 1225.448 kb on + strand, within Pf6N2E2_1051 at 1225.469 kb on - strand, within Pf6N2E2_1051 at 1225.469 kb on - strand, within Pf6N2E2_1051 at 1225.543 kb on + strand, within Pf6N2E2_1051 at 1225.566 kb on + strand, within Pf6N2E2_1051 at 1225.567 kb on - strand, within Pf6N2E2_1051 at 1225.567 kb on - strand, within Pf6N2E2_1051 at 1225.571 kb on - strand, within Pf6N2E2_1051 at 1225.571 kb on - strand, within Pf6N2E2_1051 at 1225.685 kb on + strand, within Pf6N2E2_1051 at 1225.686 kb on - strand, within Pf6N2E2_1051 at 1225.731 kb on - strand, within Pf6N2E2_1051 at 1225.964 kb on + strand at 1226.112 kb on + strand at 1226.112 kb on + strand at 1226.112 kb on + strand at 1226.161 kb on + strand at 1226.162 kb on - strand at 1226.167 kb on + strand at 1226.292 kb on + strand at 1226.302 kb on + strand at 1226.363 kb on - strand at 1226.368 kb on + strand at 1226.368 kb on + strand at 1226.368 kb on + strand at 1226.444 kb on + strand at 1226.449 kb on + strand at 1226.449 kb on + strand at 1226.449 kb on + strand at 1226.506 kb on - strand at 1226.510 kb on - strand at 1226.510 kb on - strand at 1226.543 kb on + strand at 1226.645 kb on + strand at 1226.668 kb on + strand at 1226.668 kb on + strand at 1226.694 kb on + strand at 1226.792 kb on + strand at 1226.792 kb on + strand at 1226.957 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction DEANONOate 0.000625 M remove 1,223,803 + +2.0 1,223,804 - -1.4 1,223,836 + -0.4 1,223,836 + +0.4 1,223,836 + -0.4 1,223,837 - -0.4 1,223,951 + -0.9 1,223,951 + +0.3 1,223,951 + -0.9 1,223,952 - +1.2 1,223,952 - +0.2 1,223,952 - +0.6 1,223,952 - +1.6 1,223,952 - +1.7 1,223,961 + +0.7 1,223,962 - +0.2 1,223,969 - -2.9 1,224,067 + Pf6N2E2_1050 0.27 -0.6 1,224,068 - Pf6N2E2_1050 0.28 -3.0 1,224,068 - Pf6N2E2_1050 0.28 -1.8 1,224,116 + Pf6N2E2_1050 0.36 +1.3 1,224,156 + Pf6N2E2_1050 0.43 -2.7 1,224,156 + Pf6N2E2_1050 0.43 +1.3 1,224,156 + Pf6N2E2_1050 0.43 +1.0 1,224,157 - Pf6N2E2_1050 0.44 -1.4 1,224,188 + Pf6N2E2_1050 0.49 -0.5 1,224,188 + Pf6N2E2_1050 0.49 -0.8 1,224,189 - Pf6N2E2_1050 0.49 +0.9 1,224,196 + Pf6N2E2_1050 0.51 -0.5 1,224,196 + Pf6N2E2_1050 0.51 -2.6 1,224,338 + Pf6N2E2_1050 0.76 -0.0 1,224,338 + Pf6N2E2_1050 0.76 +2.2 1,224,338 + Pf6N2E2_1050 0.76 -0.3 1,224,339 - Pf6N2E2_1050 0.77 +2.6 1,224,339 - Pf6N2E2_1050 0.77 -1.5 1,224,339 - Pf6N2E2_1050 0.77 -0.7 1,224,339 - Pf6N2E2_1050 0.77 +1.2 1,224,339 - Pf6N2E2_1050 0.77 -1.3 1,224,339 - Pf6N2E2_1050 0.77 +0.4 1,224,339 - Pf6N2E2_1050 0.77 -0.4 1,224,350 + Pf6N2E2_1050 0.79 +1.4 1,224,351 - Pf6N2E2_1050 0.79 +0.1 1,224,351 - Pf6N2E2_1050 0.79 +0.8 1,224,453 + +0.9 1,224,455 + -0.6 1,224,456 - -0.5 1,224,456 - +1.3 1,224,456 - +1.0 1,224,468 + +0.4 1,224,487 + +1.4 1,224,531 - -1.4 1,224,531 - -2.7 1,224,531 - +0.6 1,224,532 + +0.5 1,224,542 + -0.4 1,224,543 - -0.2 1,224,543 - +1.0 1,224,543 - +2.4 1,224,551 + +0.8 1,224,596 - +0.1 1,224,686 - +1.5 1,224,701 - +1.0 1,224,701 - +0.2 1,224,701 - -1.2 1,224,777 + -2.0 1,224,777 + +1.1 1,224,778 - -1.4 1,224,778 - +0.6 1,224,817 + +0.6 1,224,817 + -1.9 1,224,832 + Pf6N2E2_1051 0.11 +0.0 1,224,832 + Pf6N2E2_1051 0.11 +0.9 1,224,832 + Pf6N2E2_1051 0.11 -0.1 1,224,832 + Pf6N2E2_1051 0.11 -1.4 1,224,833 - Pf6N2E2_1051 0.11 -0.7 1,224,833 - Pf6N2E2_1051 0.11 -0.2 1,224,833 - Pf6N2E2_1051 0.11 -1.2 1,224,833 - Pf6N2E2_1051 0.11 -0.6 1,224,841 + Pf6N2E2_1051 0.11 -2.1 1,224,841 + Pf6N2E2_1051 0.11 +1.1 1,224,841 + Pf6N2E2_1051 0.11 -0.6 1,224,841 + Pf6N2E2_1051 0.11 +1.9 1,224,842 - Pf6N2E2_1051 0.11 +0.3 1,224,843 + Pf6N2E2_1051 0.11 +1.0 1,224,844 - Pf6N2E2_1051 0.11 -0.6 1,224,844 - Pf6N2E2_1051 0.11 +3.1 1,224,844 - -0.2 1,224,844 - Pf6N2E2_1051 0.11 -2.1 1,224,844 - Pf6N2E2_1051 0.11 +1.0 1,224,849 + Pf6N2E2_1051 0.12 +0.4 1,224,849 + Pf6N2E2_1051 0.12 +2.5 1,224,850 - Pf6N2E2_1051 0.12 -1.8 1,224,850 - Pf6N2E2_1051 0.12 +0.0 1,224,850 - Pf6N2E2_1051 0.12 -2.6 1,224,850 - Pf6N2E2_1051 0.12 -1.2 1,224,919 - Pf6N2E2_1051 0.17 +2.3 1,224,919 - Pf6N2E2_1051 0.17 +0.3 1,224,919 - Pf6N2E2_1051 0.17 -1.0 1,224,919 - Pf6N2E2_1051 0.17 -0.5 1,224,953 + Pf6N2E2_1051 0.19 +0.7 1,224,954 - Pf6N2E2_1051 0.20 +0.4 1,225,133 + Pf6N2E2_1051 0.33 -0.8 1,225,133 + Pf6N2E2_1051 0.33 -1.6 1,225,134 - Pf6N2E2_1051 0.33 -2.4 1,225,137 + Pf6N2E2_1051 0.33 +0.6 1,225,137 + +2.4 1,225,137 + Pf6N2E2_1051 0.33 -2.3 1,225,137 + Pf6N2E2_1051 0.33 -1.4 1,225,137 + Pf6N2E2_1051 0.33 -0.2 1,225,137 + Pf6N2E2_1051 0.33 +0.8 1,225,137 + Pf6N2E2_1051 0.33 +0.2 1,225,138 - Pf6N2E2_1051 0.33 -0.5 1,225,381 - Pf6N2E2_1051 0.51 +0.0 1,225,381 - Pf6N2E2_1051 0.51 -0.3 1,225,381 - Pf6N2E2_1051 0.51 -0.6 1,225,381 - Pf6N2E2_1051 0.51 -1.1 1,225,432 - Pf6N2E2_1051 0.55 +0.7 1,225,448 + Pf6N2E2_1051 0.56 +0.4 1,225,469 - Pf6N2E2_1051 0.58 -0.3 1,225,469 - Pf6N2E2_1051 0.58 -2.7 1,225,543 + Pf6N2E2_1051 0.63 +0.6 1,225,566 + Pf6N2E2_1051 0.65 +0.1 1,225,567 - Pf6N2E2_1051 0.65 -0.8 1,225,567 - Pf6N2E2_1051 0.65 -1.7 1,225,571 - Pf6N2E2_1051 0.65 -1.8 1,225,571 - Pf6N2E2_1051 0.65 +1.2 1,225,685 + Pf6N2E2_1051 0.73 -0.6 1,225,686 - Pf6N2E2_1051 0.74 -0.5 1,225,731 - Pf6N2E2_1051 0.77 +2.2 1,225,964 + +0.9 1,226,112 + +0.1 1,226,112 + +0.4 1,226,112 + -0.9 1,226,161 + -0.6 1,226,162 - +0.6 1,226,167 + -0.4 1,226,292 + -3.2 1,226,302 + +1.1 1,226,363 - +2.5 1,226,368 + +0.8 1,226,368 + -0.3 1,226,368 + +0.1 1,226,444 + -0.2 1,226,449 + -3.1 1,226,449 + +0.4 1,226,449 + -0.9 1,226,506 - -1.7 1,226,510 - -3.4 1,226,510 - -1.2 1,226,543 + -0.7 1,226,645 + +1.6 1,226,668 + -0.4 1,226,668 + +0.3 1,226,694 + -0.2 1,226,792 + +1.6 1,226,792 + -0.0 1,226,957 + +1.7
Or see this region's nucleotide sequence