Experiment: DEANONOate 0.000625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5823 and Pf6N2E2_5824 are separated by 34 nucleotides Pf6N2E2_5824 and Pf6N2E2_5825 are separated by 204 nucleotides Pf6N2E2_5825 and Pf6N2E2_5826 are separated by 95 nucleotides Pf6N2E2_5826 and Pf6N2E2_5827 are separated by 108 nucleotides
Pf6N2E2_5823 - Nucleoside:H+ symporter:Major facilitator superfamily, at 6,641,030 to 6,642,175
_5823
Pf6N2E2_5824 - 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (EC 1.13.11.54), at 6,642,210 to 6,642,743
_5824
Pf6N2E2_5825 - FIG00958237: hypothetical protein, at 6,642,948 to 6,643,226
_5825
Pf6N2E2_5826 - hypothetical protein, at 6,643,322 to 6,643,471
_5826
Pf6N2E2_5827 - Acetoacetyl-CoA synthetase (EC 6.2.1.16) / Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 6,643,580 to 6,645,418
_5827
Position (kb)
6642
6643
6644 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 6642.018 kb on - strand, within Pf6N2E2_5823 at 6642.059 kb on + strand, within Pf6N2E2_5823 at 6642.060 kb on - strand, within Pf6N2E2_5823 at 6642.060 kb on - strand, within Pf6N2E2_5823 at 6642.070 kb on + strand at 6642.070 kb on + strand at 6642.070 kb on + strand at 6642.070 kb on + strand at 6642.070 kb on + strand at 6642.071 kb on - strand at 6642.071 kb on - strand at 6642.154 kb on + strand at 6642.154 kb on + strand at 6642.154 kb on + strand at 6642.154 kb on + strand at 6642.154 kb on + strand at 6642.154 kb on + strand at 6642.154 kb on + strand at 6642.154 kb on + strand at 6642.154 kb on + strand at 6642.154 kb on + strand at 6642.154 kb on + strand at 6642.154 kb on + strand at 6642.154 kb on + strand at 6642.155 kb on - strand at 6642.155 kb on - strand at 6642.155 kb on - strand at 6642.155 kb on - strand at 6642.155 kb on - strand at 6642.155 kb on - strand at 6642.155 kb on - strand at 6642.155 kb on - strand at 6642.155 kb on - strand at 6642.189 kb on + strand at 6642.228 kb on + strand at 6642.229 kb on - strand at 6642.308 kb on + strand, within Pf6N2E2_5824 at 6642.308 kb on + strand, within Pf6N2E2_5824 at 6642.382 kb on - strand, within Pf6N2E2_5824 at 6642.420 kb on + strand, within Pf6N2E2_5824 at 6642.420 kb on + strand, within Pf6N2E2_5824 at 6642.494 kb on + strand, within Pf6N2E2_5824 at 6642.558 kb on + strand, within Pf6N2E2_5824 at 6642.558 kb on + strand, within Pf6N2E2_5824 at 6642.558 kb on + strand, within Pf6N2E2_5824 at 6642.558 kb on + strand, within Pf6N2E2_5824 at 6642.558 kb on + strand, within Pf6N2E2_5824 at 6642.558 kb on + strand, within Pf6N2E2_5824 at 6642.558 kb on + strand, within Pf6N2E2_5824 at 6642.558 kb on + strand, within Pf6N2E2_5824 at 6642.558 kb on + strand, within Pf6N2E2_5824 at 6642.558 kb on + strand, within Pf6N2E2_5824 at 6642.558 kb on + strand, within Pf6N2E2_5824 at 6642.558 kb on + strand, within Pf6N2E2_5824 at 6642.559 kb on - strand, within Pf6N2E2_5824 at 6642.559 kb on - strand, within Pf6N2E2_5824 at 6642.559 kb on - strand, within Pf6N2E2_5824 at 6642.559 kb on - strand, within Pf6N2E2_5824 at 6642.559 kb on - strand, within Pf6N2E2_5824 at 6642.559 kb on - strand, within Pf6N2E2_5824 at 6642.559 kb on - strand, within Pf6N2E2_5824 at 6642.559 kb on - strand, within Pf6N2E2_5824 at 6642.559 kb on - strand, within Pf6N2E2_5824 at 6642.559 kb on - strand, within Pf6N2E2_5824 at 6642.559 kb on - strand, within Pf6N2E2_5824 at 6642.564 kb on + strand, within Pf6N2E2_5824 at 6642.564 kb on + strand, within Pf6N2E2_5824 at 6642.564 kb on + strand, within Pf6N2E2_5824 at 6642.611 kb on + strand, within Pf6N2E2_5824 at 6642.611 kb on + strand, within Pf6N2E2_5824 at 6642.611 kb on + strand, within Pf6N2E2_5824 at 6642.710 kb on + strand at 6642.711 kb on - strand at 6642.711 kb on - strand at 6642.780 kb on + strand at 6642.780 kb on + strand at 6642.780 kb on + strand at 6642.780 kb on + strand at 6642.780 kb on + strand at 6642.780 kb on + strand at 6642.780 kb on + strand at 6642.780 kb on + strand at 6642.780 kb on + strand at 6642.781 kb on - strand at 6642.781 kb on - strand at 6642.781 kb on - strand at 6642.782 kb on + strand at 6642.830 kb on + strand at 6642.830 kb on + strand at 6642.830 kb on + strand at 6642.830 kb on + strand at 6642.830 kb on + strand at 6642.830 kb on + strand at 6642.830 kb on + strand at 6642.830 kb on + strand at 6642.831 kb on - strand at 6642.831 kb on - strand at 6642.957 kb on - strand at 6642.961 kb on + strand at 6642.961 kb on + strand at 6642.961 kb on + strand at 6642.961 kb on + strand at 6642.962 kb on - strand at 6642.962 kb on - strand at 6642.962 kb on - strand at 6642.962 kb on - strand at 6642.962 kb on - strand at 6643.093 kb on + strand, within Pf6N2E2_5825 at 6643.094 kb on - strand, within Pf6N2E2_5825 at 6643.127 kb on + strand, within Pf6N2E2_5825 at 6643.130 kb on + strand, within Pf6N2E2_5825 at 6643.131 kb on - strand, within Pf6N2E2_5825 at 6643.183 kb on + strand, within Pf6N2E2_5825 at 6643.184 kb on - strand, within Pf6N2E2_5825 at 6643.184 kb on - strand, within Pf6N2E2_5825 at 6643.184 kb on - strand, within Pf6N2E2_5825 at 6643.226 kb on + strand at 6643.226 kb on + strand at 6643.226 kb on + strand at 6643.227 kb on - strand at 6643.365 kb on + strand, within Pf6N2E2_5826 at 6643.487 kb on + strand at 6643.487 kb on + strand at 6643.488 kb on - strand at 6643.488 kb on - strand at 6643.488 kb on - strand at 6643.509 kb on + strand at 6643.589 kb on - strand at 6643.606 kb on + strand at 6643.606 kb on + strand at 6643.627 kb on + strand at 6643.628 kb on - strand at 6643.772 kb on + strand, within Pf6N2E2_5827 at 6643.773 kb on - strand, within Pf6N2E2_5827 at 6643.773 kb on - strand, within Pf6N2E2_5827 at 6643.773 kb on - strand, within Pf6N2E2_5827 at 6643.793 kb on + strand, within Pf6N2E2_5827 at 6643.794 kb on - strand, within Pf6N2E2_5827 at 6643.832 kb on + strand, within Pf6N2E2_5827 at 6643.832 kb on + strand, within Pf6N2E2_5827 at 6644.052 kb on + strand, within Pf6N2E2_5827 at 6644.052 kb on + strand, within Pf6N2E2_5827 at 6644.052 kb on + strand, within Pf6N2E2_5827 at 6644.052 kb on + strand, within Pf6N2E2_5827 at 6644.053 kb on - strand, within Pf6N2E2_5827 at 6644.053 kb on - strand, within Pf6N2E2_5827
Per-strain Table
Position Strand Gene LocusTag Fraction DEANONOate 0.000625 M remove 6,642,018 - Pf6N2E2_5823 0.86 +0.6 6,642,059 + Pf6N2E2_5823 0.90 -0.3 6,642,060 - Pf6N2E2_5823 0.90 -1.8 6,642,060 - Pf6N2E2_5823 0.90 -4.0 6,642,070 + +1.6 6,642,070 + +0.3 6,642,070 + -0.6 6,642,070 + -0.1 6,642,070 + +0.2 6,642,071 - -0.2 6,642,071 - -0.4 6,642,154 + +0.6 6,642,154 + +2.4 6,642,154 + +1.1 6,642,154 + +1.9 6,642,154 + +0.4 6,642,154 + +0.3 6,642,154 + -3.4 6,642,154 + -1.2 6,642,154 + +0.6 6,642,154 + -1.7 6,642,154 + -2.5 6,642,154 + +2.1 6,642,154 + -1.6 6,642,155 - -0.8 6,642,155 - -1.8 6,642,155 - -3.2 6,642,155 - -0.8 6,642,155 - -0.6 6,642,155 - +1.2 6,642,155 - +1.1 6,642,155 - +0.8 6,642,155 - +0.9 6,642,189 + -0.9 6,642,228 + +0.2 6,642,229 - -2.3 6,642,308 + Pf6N2E2_5824 0.18 +1.2 6,642,308 + Pf6N2E2_5824 0.18 +1.2 6,642,382 - Pf6N2E2_5824 0.32 +1.1 6,642,420 + Pf6N2E2_5824 0.39 -0.3 6,642,420 + Pf6N2E2_5824 0.39 +1.0 6,642,494 + Pf6N2E2_5824 0.53 +1.0 6,642,558 + Pf6N2E2_5824 0.65 +0.4 6,642,558 + Pf6N2E2_5824 0.65 -0.8 6,642,558 + Pf6N2E2_5824 0.65 -0.8 6,642,558 + Pf6N2E2_5824 0.65 +1.9 6,642,558 + Pf6N2E2_5824 0.65 +0.7 6,642,558 + Pf6N2E2_5824 0.65 +2.2 6,642,558 + Pf6N2E2_5824 0.65 -1.2 6,642,558 + Pf6N2E2_5824 0.65 +0.5 6,642,558 + Pf6N2E2_5824 0.65 -1.3 6,642,558 + Pf6N2E2_5824 0.65 -1.8 6,642,558 + Pf6N2E2_5824 0.65 -0.1 6,642,558 + Pf6N2E2_5824 0.65 -0.4 6,642,559 - Pf6N2E2_5824 0.65 -0.4 6,642,559 - Pf6N2E2_5824 0.65 +2.1 6,642,559 - Pf6N2E2_5824 0.65 -1.8 6,642,559 - Pf6N2E2_5824 0.65 +2.3 6,642,559 - Pf6N2E2_5824 0.65 -1.4 6,642,559 - Pf6N2E2_5824 0.65 -1.6 6,642,559 - Pf6N2E2_5824 0.65 +0.0 6,642,559 - Pf6N2E2_5824 0.65 -2.8 6,642,559 - Pf6N2E2_5824 0.65 +1.3 6,642,559 - Pf6N2E2_5824 0.65 +1.1 6,642,559 - Pf6N2E2_5824 0.65 -0.0 6,642,564 + Pf6N2E2_5824 0.66 -0.5 6,642,564 + Pf6N2E2_5824 0.66 +0.6 6,642,564 + Pf6N2E2_5824 0.66 -0.4 6,642,611 + Pf6N2E2_5824 0.75 +0.6 6,642,611 + Pf6N2E2_5824 0.75 -0.3 6,642,611 + Pf6N2E2_5824 0.75 +0.4 6,642,710 + -2.2 6,642,711 - +0.8 6,642,711 - -0.2 6,642,780 + +2.1 6,642,780 + -0.6 6,642,780 + +0.3 6,642,780 + +0.2 6,642,780 + +0.1 6,642,780 + +0.2 6,642,780 + -1.0 6,642,780 + -1.8 6,642,780 + -1.3 6,642,781 - +0.5 6,642,781 - -2.5 6,642,781 - +1.3 6,642,782 + -0.3 6,642,830 + -2.4 6,642,830 + +0.9 6,642,830 + -0.6 6,642,830 + -0.9 6,642,830 + -0.1 6,642,830 + +0.3 6,642,830 + -1.4 6,642,830 + -1.8 6,642,831 - +3.6 6,642,831 - -0.2 6,642,957 - +0.2 6,642,961 + -0.3 6,642,961 + -1.4 6,642,961 + -0.2 6,642,961 + -1.9 6,642,962 - -0.2 6,642,962 - -0.2 6,642,962 - -3.6 6,642,962 - -3.9 6,642,962 - -1.2 6,643,093 + Pf6N2E2_5825 0.52 -2.5 6,643,094 - Pf6N2E2_5825 0.52 -2.0 6,643,127 + Pf6N2E2_5825 0.64 -0.6 6,643,130 + Pf6N2E2_5825 0.65 -1.4 6,643,131 - Pf6N2E2_5825 0.66 -1.8 6,643,183 + Pf6N2E2_5825 0.84 -2.2 6,643,184 - Pf6N2E2_5825 0.85 -0.7 6,643,184 - Pf6N2E2_5825 0.85 -2.6 6,643,184 - Pf6N2E2_5825 0.85 +0.1 6,643,226 + -1.2 6,643,226 + -1.7 6,643,226 + -3.0 6,643,227 - -2.0 6,643,365 + Pf6N2E2_5826 0.29 -2.1 6,643,487 + -2.0 6,643,487 + -1.8 6,643,488 - -1.9 6,643,488 - -0.2 6,643,488 - -2.0 6,643,509 + +0.3 6,643,589 - +0.2 6,643,606 + -2.3 6,643,606 + -0.7 6,643,627 + +0.6 6,643,628 - +1.6 6,643,772 + Pf6N2E2_5827 0.10 -1.6 6,643,773 - Pf6N2E2_5827 0.10 +0.5 6,643,773 - Pf6N2E2_5827 0.10 -1.4 6,643,773 - Pf6N2E2_5827 0.10 -0.9 6,643,793 + Pf6N2E2_5827 0.12 -1.9 6,643,794 - Pf6N2E2_5827 0.12 -0.0 6,643,832 + Pf6N2E2_5827 0.14 -3.0 6,643,832 + Pf6N2E2_5827 0.14 -2.2 6,644,052 + Pf6N2E2_5827 0.26 -2.0 6,644,052 + Pf6N2E2_5827 0.26 -0.6 6,644,052 + Pf6N2E2_5827 0.26 +0.8 6,644,052 + Pf6N2E2_5827 0.26 -0.8 6,644,053 - Pf6N2E2_5827 0.26 +1.8 6,644,053 - Pf6N2E2_5827 0.26 -0.4
Or see this region's nucleotide sequence