Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_558

Experiment: DEANONOate 0.000625 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_555 and Pf6N2E2_556 are separated by 83 nucleotidesPf6N2E2_556 and Pf6N2E2_557 are separated by 24 nucleotidesPf6N2E2_557 and Pf6N2E2_558 are separated by 63 nucleotidesPf6N2E2_558 and Pf6N2E2_559 are separated by 18 nucleotidesPf6N2E2_559 and Pf6N2E2_560 overlap by 4 nucleotides Pf6N2E2_555 - Uncharacterized protein ImpB, at 627,315 to 627,875 _555 Pf6N2E2_556 - hypothetical protein, at 627,959 to 628,075 _556 Pf6N2E2_557 - Uncharacterized protein homologous to Paeru. PA2364, at 628,100 to 628,549 _557 Pf6N2E2_558 - Uncharacterized protein ImpJ/VasE, at 628,613 to 629,944 _558 Pf6N2E2_559 - Inner membrane protein DotU, at 629,963 to 630,727 _559 Pf6N2E2_560 - IcmF-related protein, at 630,724 to 634,548 _560 Position (kb) 628 629 630Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 627.814 kb on + strand, within Pf6N2E2_555at 627.814 kb on + strandat 627.814 kb on + strand, within Pf6N2E2_555at 627.814 kb on + strand, within Pf6N2E2_555at 627.815 kb on - strand, within Pf6N2E2_555at 627.815 kb on - strand, within Pf6N2E2_555at 627.864 kb on - strandat 628.023 kb on + strand, within Pf6N2E2_556at 628.036 kb on - strand, within Pf6N2E2_556at 628.445 kb on + strand, within Pf6N2E2_557at 628.445 kb on + strand, within Pf6N2E2_557at 628.445 kb on + strand, within Pf6N2E2_557at 628.445 kb on + strand, within Pf6N2E2_557at 628.445 kb on + strand, within Pf6N2E2_557at 628.445 kb on + strand, within Pf6N2E2_557at 628.445 kb on + strand, within Pf6N2E2_557at 628.445 kb on + strand, within Pf6N2E2_557at 628.446 kb on - strand, within Pf6N2E2_557at 628.446 kb on - strand, within Pf6N2E2_557at 628.446 kb on - strand, within Pf6N2E2_557at 628.446 kb on - strand, within Pf6N2E2_557at 628.448 kb on - strand, within Pf6N2E2_557at 628.448 kb on - strand, within Pf6N2E2_557at 628.448 kb on - strand, within Pf6N2E2_557at 628.612 kb on + strandat 628.613 kb on - strandat 628.925 kb on + strand, within Pf6N2E2_558at 628.925 kb on + strand, within Pf6N2E2_558at 628.925 kb on + strand, within Pf6N2E2_558at 628.926 kb on - strand, within Pf6N2E2_558at 629.156 kb on + strand, within Pf6N2E2_558at 629.393 kb on + strand, within Pf6N2E2_558at 629.394 kb on - strand, within Pf6N2E2_558at 629.786 kb on + strand, within Pf6N2E2_558at 629.786 kb on + strand, within Pf6N2E2_558at 629.787 kb on - strand, within Pf6N2E2_558at 629.962 kb on + strandat 629.962 kb on + strandat 629.962 kb on + strandat 629.962 kb on + strandat 629.962 kb on + strandat 629.962 kb on + strandat 629.962 kb on + strandat 629.962 kb on + strandat 629.963 kb on - strandat 630.090 kb on + strand, within Pf6N2E2_559at 630.201 kb on - strand, within Pf6N2E2_559at 630.201 kb on - strand, within Pf6N2E2_559at 630.293 kb on + strand, within Pf6N2E2_559at 630.293 kb on + strand, within Pf6N2E2_559at 630.293 kb on + strand, within Pf6N2E2_559at 630.293 kb on + strand, within Pf6N2E2_559at 630.293 kb on + strand, within Pf6N2E2_559at 630.294 kb on - strand, within Pf6N2E2_559at 630.294 kb on - strand, within Pf6N2E2_559at 630.294 kb on - strand, within Pf6N2E2_559at 630.294 kb on - strand, within Pf6N2E2_559at 630.294 kb on - strand, within Pf6N2E2_559at 630.294 kb on - strand, within Pf6N2E2_559at 630.294 kb on - strand, within Pf6N2E2_559at 630.294 kb on - strand, within Pf6N2E2_559at 630.343 kb on - strand, within Pf6N2E2_559at 630.387 kb on - strand, within Pf6N2E2_559at 630.503 kb on + strand, within Pf6N2E2_559at 630.503 kb on + strand, within Pf6N2E2_559at 630.503 kb on + strand, within Pf6N2E2_559at 630.839 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction DEANONOate 0.000625 M
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627,814 + Pf6N2E2_555 0.89 +0.8
627,814 + +0.5
627,814 + Pf6N2E2_555 0.89 +0.4
627,814 + Pf6N2E2_555 0.89 -3.9
627,815 - Pf6N2E2_555 0.89 +0.2
627,815 - Pf6N2E2_555 0.89 -0.6
627,864 - +1.8
628,023 + Pf6N2E2_556 0.55 +1.3
628,036 - Pf6N2E2_556 0.66 -1.3
628,445 + Pf6N2E2_557 0.77 +1.8
628,445 + Pf6N2E2_557 0.77 -2.8
628,445 + Pf6N2E2_557 0.77 -1.1
628,445 + Pf6N2E2_557 0.77 -1.0
628,445 + Pf6N2E2_557 0.77 +0.6
628,445 + Pf6N2E2_557 0.77 -0.6
628,445 + Pf6N2E2_557 0.77 -0.6
628,445 + Pf6N2E2_557 0.77 -0.7
628,446 - Pf6N2E2_557 0.77 -1.0
628,446 - Pf6N2E2_557 0.77 -0.6
628,446 - Pf6N2E2_557 0.77 -1.6
628,446 - Pf6N2E2_557 0.77 -1.3
628,448 - Pf6N2E2_557 0.77 +1.0
628,448 - Pf6N2E2_557 0.77 +0.9
628,448 - Pf6N2E2_557 0.77 +1.0
628,612 + -1.3
628,613 - +0.0
628,925 + Pf6N2E2_558 0.23 -0.8
628,925 + Pf6N2E2_558 0.23 +0.4
628,925 + Pf6N2E2_558 0.23 -2.5
628,926 - Pf6N2E2_558 0.23 +0.4
629,156 + Pf6N2E2_558 0.41 +0.7
629,393 + Pf6N2E2_558 0.59 -0.5
629,394 - Pf6N2E2_558 0.59 -2.8
629,786 + Pf6N2E2_558 0.88 -0.5
629,786 + Pf6N2E2_558 0.88 +0.1
629,787 - Pf6N2E2_558 0.88 -0.3
629,962 + +0.8
629,962 + +0.5
629,962 + +2.2
629,962 + +0.3
629,962 + +0.4
629,962 + -0.4
629,962 + -1.4
629,962 + +0.4
629,963 - -0.3
630,090 + Pf6N2E2_559 0.17 +0.5
630,201 - Pf6N2E2_559 0.31 -0.9
630,201 - Pf6N2E2_559 0.31 -1.3
630,293 + Pf6N2E2_559 0.43 -0.6
630,293 + Pf6N2E2_559 0.43 -2.2
630,293 + Pf6N2E2_559 0.43 -0.5
630,293 + Pf6N2E2_559 0.43 -1.3
630,293 + Pf6N2E2_559 0.43 +1.0
630,294 - Pf6N2E2_559 0.43 -0.7
630,294 - Pf6N2E2_559 0.43 -1.6
630,294 - Pf6N2E2_559 0.43 -1.4
630,294 - Pf6N2E2_559 0.43 -2.8
630,294 - Pf6N2E2_559 0.43 +0.8
630,294 - Pf6N2E2_559 0.43 +0.9
630,294 - Pf6N2E2_559 0.43 -0.8
630,294 - Pf6N2E2_559 0.43 +0.7
630,343 - Pf6N2E2_559 0.50 -1.6
630,387 - Pf6N2E2_559 0.55 -1.5
630,503 + Pf6N2E2_559 0.71 +0.8
630,503 + Pf6N2E2_559 0.71 -0.1
630,503 + Pf6N2E2_559 0.71 -0.9
630,839 - -0.8

Or see this region's nucleotide sequence