Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4517

Experiment: DEANONOate 0.000625 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4515 and Pf6N2E2_4516 overlap by 4 nucleotidesPf6N2E2_4516 and Pf6N2E2_4517 overlap by 4 nucleotidesPf6N2E2_4517 and Pf6N2E2_4518 are separated by 235 nucleotidesPf6N2E2_4518 and Pf6N2E2_4519 are separated by 102 nucleotides Pf6N2E2_4515 - Putrescine transport ATP-binding protein PotG (TC 3.A.1.11.2), at 5,248,124 to 5,249,266 _4515 Pf6N2E2_4516 - Putrescine transport system permease protein PotH (TC 3.A.1.11.2), at 5,249,263 to 5,250,183 _4516 Pf6N2E2_4517 - Putrescine transport system permease protein PotI (TC 3.A.1.11.2), at 5,250,180 to 5,251,067 _4517 Pf6N2E2_4518 - Carboxymuconolactone decarboxylase family protein, at 5,251,303 to 5,251,644 _4518 Pf6N2E2_4519 - Penicillin acylase II (EC 3.5.1.11), at 5,251,747 to 5,254,155 _4519 Position (kb) 5250 5251 5252Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5249.254 kb on - strandat 5249.437 kb on + strand, within Pf6N2E2_4516at 5249.437 kb on + strand, within Pf6N2E2_4516at 5249.437 kb on + strand, within Pf6N2E2_4516at 5249.437 kb on + strand, within Pf6N2E2_4516at 5249.437 kb on + strand, within Pf6N2E2_4516at 5249.437 kb on + strand, within Pf6N2E2_4516at 5249.438 kb on - strand, within Pf6N2E2_4516at 5249.438 kb on - strand, within Pf6N2E2_4516at 5249.438 kb on - strand, within Pf6N2E2_4516at 5249.438 kb on - strand, within Pf6N2E2_4516at 5249.438 kb on - strand, within Pf6N2E2_4516at 5249.443 kb on + strand, within Pf6N2E2_4516at 5249.443 kb on + strand, within Pf6N2E2_4516at 5249.443 kb on + strand, within Pf6N2E2_4516at 5249.443 kb on + strand, within Pf6N2E2_4516at 5249.443 kb on + strand, within Pf6N2E2_4516at 5249.444 kb on - strand, within Pf6N2E2_4516at 5249.444 kb on - strand, within Pf6N2E2_4516at 5249.444 kb on - strand, within Pf6N2E2_4516at 5249.444 kb on - strand, within Pf6N2E2_4516at 5249.485 kb on + strand, within Pf6N2E2_4516at 5249.485 kb on + strand, within Pf6N2E2_4516at 5249.485 kb on + strand, within Pf6N2E2_4516at 5249.512 kb on + strand, within Pf6N2E2_4516at 5249.513 kb on - strand, within Pf6N2E2_4516at 5249.513 kb on - strand, within Pf6N2E2_4516at 5249.513 kb on - strand, within Pf6N2E2_4516at 5249.594 kb on - strand, within Pf6N2E2_4516at 5249.594 kb on - strand, within Pf6N2E2_4516at 5249.594 kb on - strand, within Pf6N2E2_4516at 5249.594 kb on - strand, within Pf6N2E2_4516at 5249.748 kb on + strand, within Pf6N2E2_4516at 5249.749 kb on - strand, within Pf6N2E2_4516at 5249.791 kb on + strand, within Pf6N2E2_4516at 5249.792 kb on - strand, within Pf6N2E2_4516at 5249.792 kb on - strand, within Pf6N2E2_4516at 5249.792 kb on - strand, within Pf6N2E2_4516at 5249.792 kb on - strand, within Pf6N2E2_4516at 5249.807 kb on - strand, within Pf6N2E2_4516at 5249.812 kb on + strand, within Pf6N2E2_4516at 5249.813 kb on - strand, within Pf6N2E2_4516at 5249.840 kb on - strand, within Pf6N2E2_4516at 5250.235 kb on - strandat 5250.261 kb on + strandat 5250.261 kb on + strandat 5250.261 kb on + strandat 5250.262 kb on - strandat 5250.319 kb on - strand, within Pf6N2E2_4517at 5250.319 kb on - strand, within Pf6N2E2_4517at 5250.385 kb on - strand, within Pf6N2E2_4517at 5250.624 kb on + strand, within Pf6N2E2_4517at 5250.625 kb on - strand, within Pf6N2E2_4517at 5250.625 kb on - strand, within Pf6N2E2_4517at 5250.625 kb on - strand, within Pf6N2E2_4517at 5250.978 kb on - strand, within Pf6N2E2_4517at 5251.066 kb on - strandat 5251.346 kb on + strand, within Pf6N2E2_4518at 5251.346 kb on + strand, within Pf6N2E2_4518at 5251.346 kb on + strand, within Pf6N2E2_4518at 5251.346 kb on + strand, within Pf6N2E2_4518at 5251.346 kb on + strand, within Pf6N2E2_4518at 5251.346 kb on + strand, within Pf6N2E2_4518at 5251.347 kb on - strand, within Pf6N2E2_4518at 5251.350 kb on + strand, within Pf6N2E2_4518at 5251.350 kb on + strand, within Pf6N2E2_4518at 5251.351 kb on - strand, within Pf6N2E2_4518at 5251.351 kb on - strand, within Pf6N2E2_4518at 5251.351 kb on - strand, within Pf6N2E2_4518at 5251.351 kb on - strand, within Pf6N2E2_4518at 5251.352 kb on + strand, within Pf6N2E2_4518at 5251.352 kb on + strand, within Pf6N2E2_4518at 5251.352 kb on + strand, within Pf6N2E2_4518at 5251.352 kb on + strand, within Pf6N2E2_4518at 5251.353 kb on - strand, within Pf6N2E2_4518at 5251.353 kb on - strand, within Pf6N2E2_4518at 5251.353 kb on - strand, within Pf6N2E2_4518at 5251.353 kb on - strand, within Pf6N2E2_4518at 5251.353 kb on - strand, within Pf6N2E2_4518at 5251.353 kb on - strand, within Pf6N2E2_4518at 5251.353 kb on - strand, within Pf6N2E2_4518at 5251.444 kb on + strand, within Pf6N2E2_4518at 5251.445 kb on - strand, within Pf6N2E2_4518at 5251.556 kb on + strand, within Pf6N2E2_4518at 5251.556 kb on + strand, within Pf6N2E2_4518at 5251.556 kb on + strand, within Pf6N2E2_4518at 5251.557 kb on - strand, within Pf6N2E2_4518at 5251.615 kb on - strandat 5251.615 kb on - strandat 5251.636 kb on - strandat 5251.636 kb on - strandat 5251.687 kb on + strandat 5251.687 kb on + strandat 5251.688 kb on - strandat 5251.688 kb on - strandat 5251.688 kb on - strandat 5251.722 kb on + strandat 5251.734 kb on - strandat 5251.771 kb on + strandat 5251.772 kb on - strandat 5251.832 kb on + strandat 5251.832 kb on + strandat 5251.832 kb on + strandat 5251.833 kb on - strandat 5251.847 kb on + strandat 5251.847 kb on + strandat 5251.847 kb on + strandat 5251.847 kb on + strandat 5251.848 kb on - strandat 5251.848 kb on - strandat 5251.848 kb on - strandat 5251.869 kb on - strandat 5251.956 kb on + strandat 5251.967 kb on + strandat 5251.967 kb on + strandat 5251.967 kb on + strandat 5251.967 kb on + strandat 5251.967 kb on + strandat 5251.967 kb on + strandat 5251.967 kb on + strandat 5251.967 kb on + strandat 5251.967 kb on + strandat 5251.967 kb on + strandat 5251.968 kb on - strandat 5251.968 kb on - strandat 5251.968 kb on - strandat 5251.968 kb on - strandat 5251.968 kb on - strandat 5251.968 kb on - strandat 5251.968 kb on - strandat 5251.968 kb on - strandat 5251.968 kb on - strandat 5251.968 kb on - strandat 5251.968 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction DEANONOate 0.000625 M
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5,249,254 - +0.7
5,249,437 + Pf6N2E2_4516 0.19 -1.9
5,249,437 + Pf6N2E2_4516 0.19 -1.0
5,249,437 + Pf6N2E2_4516 0.19 +0.3
5,249,437 + Pf6N2E2_4516 0.19 +0.8
5,249,437 + Pf6N2E2_4516 0.19 +0.4
5,249,437 + Pf6N2E2_4516 0.19 +0.4
5,249,438 - Pf6N2E2_4516 0.19 +1.4
5,249,438 - Pf6N2E2_4516 0.19 +0.3
5,249,438 - Pf6N2E2_4516 0.19 +0.3
5,249,438 - Pf6N2E2_4516 0.19 +0.0
5,249,438 - Pf6N2E2_4516 0.19 +0.3
5,249,443 + Pf6N2E2_4516 0.20 +0.4
5,249,443 + Pf6N2E2_4516 0.20 -1.1
5,249,443 + Pf6N2E2_4516 0.20 -0.1
5,249,443 + Pf6N2E2_4516 0.20 -1.0
5,249,443 + Pf6N2E2_4516 0.20 +2.2
5,249,444 - Pf6N2E2_4516 0.20 +0.7
5,249,444 - Pf6N2E2_4516 0.20 +0.7
5,249,444 - Pf6N2E2_4516 0.20 +0.1
5,249,444 - Pf6N2E2_4516 0.20 +0.2
5,249,485 + Pf6N2E2_4516 0.24 +1.7
5,249,485 + Pf6N2E2_4516 0.24 -2.7
5,249,485 + Pf6N2E2_4516 0.24 +0.1
5,249,512 + Pf6N2E2_4516 0.27 +0.6
5,249,513 - Pf6N2E2_4516 0.27 +0.2
5,249,513 - Pf6N2E2_4516 0.27 +1.4
5,249,513 - Pf6N2E2_4516 0.27 -0.6
5,249,594 - Pf6N2E2_4516 0.36 -0.5
5,249,594 - Pf6N2E2_4516 0.36 +0.2
5,249,594 - Pf6N2E2_4516 0.36 -0.4
5,249,594 - Pf6N2E2_4516 0.36 -0.3
5,249,748 + Pf6N2E2_4516 0.53 -0.1
5,249,749 - Pf6N2E2_4516 0.53 +0.5
5,249,791 + Pf6N2E2_4516 0.57 -0.8
5,249,792 - Pf6N2E2_4516 0.57 -0.4
5,249,792 - Pf6N2E2_4516 0.57 -1.1
5,249,792 - Pf6N2E2_4516 0.57 -0.1
5,249,792 - Pf6N2E2_4516 0.57 -1.4
5,249,807 - Pf6N2E2_4516 0.59 -0.1
5,249,812 + Pf6N2E2_4516 0.60 -0.0
5,249,813 - Pf6N2E2_4516 0.60 -0.1
5,249,840 - Pf6N2E2_4516 0.63 -1.0
5,250,235 - +0.3
5,250,261 + -0.9
5,250,261 + +1.1
5,250,261 + -1.7
5,250,262 - -0.5
5,250,319 - Pf6N2E2_4517 0.16 -0.9
5,250,319 - Pf6N2E2_4517 0.16 -2.6
5,250,385 - Pf6N2E2_4517 0.23 -1.6
5,250,624 + Pf6N2E2_4517 0.50 -2.8
5,250,625 - Pf6N2E2_4517 0.50 -3.2
5,250,625 - Pf6N2E2_4517 0.50 -3.0
5,250,625 - Pf6N2E2_4517 0.50 -1.9
5,250,978 - Pf6N2E2_4517 0.90 +0.4
5,251,066 - -0.2
5,251,346 + Pf6N2E2_4518 0.13 +0.6
5,251,346 + Pf6N2E2_4518 0.13 +0.4
5,251,346 + Pf6N2E2_4518 0.13 -2.9
5,251,346 + Pf6N2E2_4518 0.13 +0.7
5,251,346 + Pf6N2E2_4518 0.13 +0.3
5,251,346 + Pf6N2E2_4518 0.13 +0.3
5,251,347 - Pf6N2E2_4518 0.13 +0.2
5,251,350 + Pf6N2E2_4518 0.14 +0.4
5,251,350 + Pf6N2E2_4518 0.14 +0.1
5,251,351 - Pf6N2E2_4518 0.14 -1.1
5,251,351 - Pf6N2E2_4518 0.14 -0.7
5,251,351 - Pf6N2E2_4518 0.14 -3.4
5,251,351 - Pf6N2E2_4518 0.14 -0.7
5,251,352 + Pf6N2E2_4518 0.14 +0.4
5,251,352 + Pf6N2E2_4518 0.14 +0.0
5,251,352 + Pf6N2E2_4518 0.14 +1.1
5,251,352 + Pf6N2E2_4518 0.14 -0.6
5,251,353 - Pf6N2E2_4518 0.15 +0.5
5,251,353 - Pf6N2E2_4518 0.15 -2.2
5,251,353 - Pf6N2E2_4518 0.15 -2.9
5,251,353 - Pf6N2E2_4518 0.15 -1.1
5,251,353 - Pf6N2E2_4518 0.15 +1.4
5,251,353 - Pf6N2E2_4518 0.15 +1.2
5,251,353 - Pf6N2E2_4518 0.15 +0.8
5,251,444 + Pf6N2E2_4518 0.41 -0.5
5,251,445 - Pf6N2E2_4518 0.42 -2.9
5,251,556 + Pf6N2E2_4518 0.74 -0.8
5,251,556 + Pf6N2E2_4518 0.74 +0.4
5,251,556 + Pf6N2E2_4518 0.74 +0.6
5,251,557 - Pf6N2E2_4518 0.74 -1.2
5,251,615 - +0.2
5,251,615 - +1.7
5,251,636 - -1.2
5,251,636 - -0.0
5,251,687 + +0.2
5,251,687 + -1.2
5,251,688 - -1.8
5,251,688 - +0.7
5,251,688 - +0.1
5,251,722 + -3.6
5,251,734 - +1.3
5,251,771 + +0.3
5,251,772 - +0.3
5,251,832 + -1.0
5,251,832 + +1.3
5,251,832 + +1.1
5,251,833 - -0.3
5,251,847 + +1.3
5,251,847 + +0.8
5,251,847 + -1.3
5,251,847 + +0.8
5,251,848 - +1.1
5,251,848 - +0.1
5,251,848 - +0.6
5,251,869 - -0.1
5,251,956 + +0.1
5,251,967 + -3.6
5,251,967 + -1.1
5,251,967 + +0.2
5,251,967 + +0.4
5,251,967 + +0.8
5,251,967 + -0.6
5,251,967 + +0.9
5,251,967 + +1.8
5,251,967 + +0.3
5,251,967 + +0.6
5,251,968 - -2.2
5,251,968 - +1.7
5,251,968 - +0.8
5,251,968 - -0.1
5,251,968 - -0.6
5,251,968 - -1.7
5,251,968 - +1.4
5,251,968 - +1.8
5,251,968 - +0.1
5,251,968 - +1.1
5,251,968 - +1.6

Or see this region's nucleotide sequence