Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_424

Experiment: DEANONOate 0.000625 M

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	DEANONOate 0.000625 M
491,729	+		Pf6N2E2_422	0.90	-2.0
491,730	-		Pf6N2E2_422	0.90	+0.3
491,786	+				+1.6
491,787	-				+2.0
491,817	-				+1.5
491,819	+				-2.4
491,820	-				-0.2
491,861	+				+0.1
491,877	+				-3.4
491,877	+				+0.7
491,877	+				-0.1
491,877	+				+0.6
491,878	-				-3.3
491,878	-				-1.4
491,878	-				+2.3
491,878	-				+1.5
492,279	+		Pf6N2E2_423	0.56	+0.6
492,486	+		Pf6N2E2_423	0.85	-0.4
492,526	+				-1.1
492,526	+				-0.3
492,527	-				-0.8
492,527	-				-0.5
492,527	-				+0.2
492,584	+				+0.6
492,584	+				-0.2
492,585	-				-2.3
492,585	-				+2.1
492,585	-				+0.1
492,585	-				+0.0
492,660	+				-4.2
492,714	+				-2.0
492,715	-				+0.8
492,746	+				+0.5
492,746	+				-0.7
492,747	-				-2.5
492,747	-				+0.7
492,747	-				+0.1
492,747	-				+0.4
493,143	-		Pf6N2E2_424	0.31	-2.0
493,143	-		Pf6N2E2_424	0.31	-2.5
493,348	+		Pf6N2E2_424	0.46	-1.6
493,348	+		Pf6N2E2_424	0.46	+0.3
493,349	-		Pf6N2E2_424	0.46	-3.8
493,470	+		Pf6N2E2_424	0.55	-1.7
493,471	-		Pf6N2E2_424	0.55	-3.6
493,545	+		Pf6N2E2_424	0.60	-1.7
493,546	-		Pf6N2E2_424	0.60	-2.6
493,589	+		Pf6N2E2_424	0.63	-0.2
493,590	-		Pf6N2E2_424	0.63	-0.3
493,590	-		Pf6N2E2_424	0.63	-3.2
493,590	-		Pf6N2E2_424	0.63	-2.6
493,595	+		Pf6N2E2_424	0.64	-0.8
493,595	+		Pf6N2E2_424	0.64	-2.8
493,595	+		Pf6N2E2_424	0.64	-2.1
493,694	+		Pf6N2E2_424	0.71	+0.7
493,695	-		Pf6N2E2_424	0.71	-0.2
493,695	-		Pf6N2E2_424	0.71	-2.0
493,901	+		Pf6N2E2_424	0.86	-1.3
493,902	-		Pf6N2E2_424	0.86	+2.0
493,982	+				-2.5
493,982	+				-3.2
493,982	+				-0.8
493,982	+				-1.0
493,982	+				-2.0
493,982	+				-0.9
493,982	+				-2.4
493,982	+				+0.5
493,982	+				-2.0
493,983	-				-1.9
493,983	-				-2.0
493,986	+				-2.5
494,074	+				-1.7
494,074	+				-2.2
494,074	+				-1.7
494,074	+				-1.5
494,074	+				-1.0
494,074	+				-0.6
494,075	-				-0.9
494,075	-				-0.5
494,075	-				-2.2
494,241	+				-0.2
494,241	+				-3.4
494,241	+				-0.6
494,242	-				+1.3
494,245	+				-0.3
494,245	+				-0.1
494,245	+				-0.8
494,245	+				+1.3
494,245	+				-2.8
494,245	+				+0.0
494,246	-				-1.7
494,246	-				-2.9
494,246	-				-0.3
494,246	-				-0.3
494,246	-				-0.2
494,289	+				-0.2
494,289	+				+0.6
494,290	-				+1.0
494,290	-				+0.4
494,290	-				-0.5
494,342	+				-2.8
494,343	-				-3.1
494,343	-				+1.5
494,343	-				-0.7
494,519	+		Pf6N2E2_425	0.39	-1.7
494,520	-		Pf6N2E2_425	0.40	-0.7
494,555	+		Pf6N2E2_425	0.46	-0.2
494,664	-		Pf6N2E2_425	0.66	-1.5
494,664	-		Pf6N2E2_425	0.66	-2.8
494,828	+				+1.0
495,046	+		Pf6N2E2_426	0.44	-0.7
495,047	-		Pf6N2E2_426	0.44	+0.5
495,047	-		Pf6N2E2_426	0.44	+1.4
495,047	-		Pf6N2E2_426	0.44	-2.8