Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_413

Experiment: DEANONOate 0.000625 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_410 and Pf6N2E2_411 are separated by 76 nucleotidesPf6N2E2_411 and Pf6N2E2_412 are separated by 183 nucleotidesPf6N2E2_412 and Pf6N2E2_413 are separated by 32 nucleotidesPf6N2E2_413 and Pf6N2E2_414 are separated by 2 nucleotidesPf6N2E2_414 and Pf6N2E2_415 are separated by 17 nucleotides Pf6N2E2_410 - hypothetical protein, at 480,118 to 480,660 _410 Pf6N2E2_411 - Nitrogen regulation protein NR(I), at 480,737 to 480,892 _411 Pf6N2E2_412 - hypothetical protein, at 481,076 to 481,336 _412 Pf6N2E2_413 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 481,369 to 482,442 _413 Pf6N2E2_414 - Chemotaxis protein CheD, at 482,445 to 482,921 _414 Pf6N2E2_415 - Chemotaxis protein methyltransferase CheR (EC 2.1.1.80), at 482,939 to 483,775 _415 Position (kb) 481 482 483Strain fitness (log2 ratio) -3 -2 -1 0 1at 480.719 kb on - strandat 481.061 kb on - strandat 481.354 kb on + strandat 481.355 kb on - strandat 481.355 kb on - strandat 481.664 kb on + strand, within Pf6N2E2_413at 481.664 kb on + strand, within Pf6N2E2_413at 482.422 kb on + strandat 482.422 kb on + strandat 482.422 kb on + strandat 482.423 kb on - strandat 482.423 kb on - strandat 482.423 kb on - strandat 482.423 kb on - strandat 482.423 kb on - strandat 482.423 kb on - strandat 482.423 kb on - strandat 482.423 kb on - strandat 482.442 kb on + strandat 482.461 kb on - strandat 482.461 kb on - strandat 482.462 kb on + strandat 482.462 kb on + strandat 482.462 kb on + strandat 482.462 kb on + strandat 482.462 kb on + strandat 482.462 kb on + strandat 482.462 kb on + strandat 482.463 kb on - strandat 482.463 kb on - strandat 482.463 kb on - strandat 482.463 kb on - strandat 482.463 kb on - strandat 482.463 kb on - strandat 482.463 kb on - strandat 482.463 kb on - strandat 482.481 kb on + strandat 482.482 kb on - strandat 482.482 kb on - strandat 482.482 kb on - strandat 482.482 kb on - strandat 482.482 kb on - strandat 482.740 kb on + strand, within Pf6N2E2_414at 482.741 kb on - strand, within Pf6N2E2_414at 482.741 kb on - strand, within Pf6N2E2_414at 482.741 kb on - strand, within Pf6N2E2_414at 482.743 kb on - strand, within Pf6N2E2_414at 482.743 kb on - strand, within Pf6N2E2_414at 482.909 kb on - strandat 482.909 kb on - strandat 482.909 kb on - strandat 483.025 kb on - strand, within Pf6N2E2_415at 483.090 kb on + strand, within Pf6N2E2_415at 483.090 kb on + strand, within Pf6N2E2_415at 483.090 kb on + strand, within Pf6N2E2_415at 483.090 kb on + strand, within Pf6N2E2_415at 483.091 kb on - strand, within Pf6N2E2_415at 483.091 kb on - strand, within Pf6N2E2_415at 483.091 kb on - strand, within Pf6N2E2_415at 483.240 kb on + strand, within Pf6N2E2_415at 483.241 kb on - strand, within Pf6N2E2_415at 483.315 kb on - strand, within Pf6N2E2_415at 483.315 kb on - strand, within Pf6N2E2_415at 483.393 kb on + strand, within Pf6N2E2_415at 483.394 kb on - strand, within Pf6N2E2_415

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Per-strain Table

Position Strand Gene LocusTag Fraction DEANONOate 0.000625 M
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480,719 - +0.4
481,061 - -2.7
481,354 + +0.7
481,355 - -1.0
481,355 - -0.5
481,664 + Pf6N2E2_413 0.27 -3.3
481,664 + Pf6N2E2_413 0.27 -2.8
482,422 + +1.2
482,422 + -1.7
482,422 + +0.8
482,423 - +0.1
482,423 - +0.3
482,423 - -0.7
482,423 - -1.1
482,423 - +0.3
482,423 - -0.0
482,423 - -0.4
482,423 - +0.4
482,442 + -0.2
482,461 - -1.4
482,461 - -0.6
482,462 + +0.1
482,462 + +1.0
482,462 + -0.8
482,462 + -0.7
482,462 + -1.3
482,462 + +0.8
482,462 + +0.5
482,463 - +0.1
482,463 - -2.7
482,463 - -0.4
482,463 - -0.6
482,463 - +0.1
482,463 - -0.1
482,463 - -1.0
482,463 - -0.3
482,481 + +1.2
482,482 - +0.3
482,482 - +0.0
482,482 - +0.2
482,482 - -3.3
482,482 - +0.3
482,740 + Pf6N2E2_414 0.62 -0.6
482,741 - Pf6N2E2_414 0.62 -2.3
482,741 - Pf6N2E2_414 0.62 -0.0
482,741 - Pf6N2E2_414 0.62 -1.0
482,743 - Pf6N2E2_414 0.62 -0.2
482,743 - Pf6N2E2_414 0.62 -3.1
482,909 - +0.7
482,909 - -0.5
482,909 - -0.7
483,025 - Pf6N2E2_415 0.10 +0.5
483,090 + Pf6N2E2_415 0.18 -0.4
483,090 + Pf6N2E2_415 0.18 +1.0
483,090 + Pf6N2E2_415 0.18 -1.6
483,090 + Pf6N2E2_415 0.18 -0.1
483,091 - Pf6N2E2_415 0.18 -0.5
483,091 - Pf6N2E2_415 0.18 +0.7
483,091 - Pf6N2E2_415 0.18 +1.3
483,240 + Pf6N2E2_415 0.36 -1.6
483,241 - Pf6N2E2_415 0.36 +1.4
483,315 - Pf6N2E2_415 0.45 +1.6
483,315 - Pf6N2E2_415 0.45 -2.8
483,393 + Pf6N2E2_415 0.54 +1.0
483,394 - Pf6N2E2_415 0.54 -1.3

Or see this region's nucleotide sequence