Experiment: DEANONOate 0.000625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3182 and Pf6N2E2_3183 are separated by 88 nucleotides Pf6N2E2_3183 and Pf6N2E2_3184 are separated by 13 nucleotides Pf6N2E2_3184 and Pf6N2E2_3185 are separated by 3 nucleotides Pf6N2E2_3185 and Pf6N2E2_3186 are separated by 50 nucleotides Pf6N2E2_3186 and Pf6N2E2_3187 are separated by 8 nucleotides Pf6N2E2_3187 and Pf6N2E2_3188 are separated by 24 nucleotides
Pf6N2E2_3182 - Nucleoside diphosphate kinase (EC 2.7.4.6), at 3,678,532 to 3,678,957
_3182
Pf6N2E2_3183 - Believed to be involved in assembly of Fe-S clusters, at 3,679,046 to 3,679,246
_3183
Pf6N2E2_3184 - Ferredoxin, 2Fe-2S, at 3,679,260 to 3,679,601
_3184
Pf6N2E2_3185 - Chaperone protein HscA, at 3,679,605 to 3,681,467
_3185
Pf6N2E2_3186 - Chaperone protein HscB, at 3,681,518 to 3,682,039
_3186
Pf6N2E2_3187 - Iron binding protein IscA for iron-sulfur cluster assembly, at 3,682,048 to 3,682,371
_3187
Pf6N2E2_3188 - Iron-sulfur cluster assembly scaffold protein IscU, at 3,682,396 to 3,682,782
_3188
Position (kb)
3679
3680
3681
3682 Strain fitness (log2 ratio)
-1
0
1
2 at 3679.006 kb on - strand at 3679.006 kb on - strand at 3679.008 kb on - strand at 3679.224 kb on - strand, within Pf6N2E2_3183 at 3679.224 kb on - strand, within Pf6N2E2_3183 at 3679.224 kb on - strand, within Pf6N2E2_3183 at 3679.224 kb on - strand, within Pf6N2E2_3183 at 3679.239 kb on + strand at 3679.240 kb on - strand at 3679.240 kb on - strand at 3679.240 kb on - strand at 3679.240 kb on - strand at 3679.240 kb on - strand at 3679.240 kb on - strand at 3679.240 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction DEANONOate 0.000625 M remove 3,679,006 - -0.1 3,679,006 - -1.3 3,679,008 - -0.9 3,679,224 - Pf6N2E2_3183 0.89 +1.7 3,679,224 - Pf6N2E2_3183 0.89 +2.1 3,679,224 - Pf6N2E2_3183 0.89 +0.6 3,679,224 - Pf6N2E2_3183 0.89 +1.3 3,679,239 + +2.7 3,679,240 - +0.2 3,679,240 - +1.0 3,679,240 - +1.9 3,679,240 - -0.2 3,679,240 - -1.2 3,679,240 - +2.1 3,679,240 - +2.7
Or see this region's nucleotide sequence