Experiment: DEANONOate 0.000625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2194 and Pf6N2E2_2195 are separated by 29 nucleotides Pf6N2E2_2195 and Pf6N2E2_2196 are separated by 122 nucleotides Pf6N2E2_2196 and Pf6N2E2_2197 are separated by 499 nucleotides
Pf6N2E2_2194 - 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) (from data) , at 2,600,323 to 2,602,272
_2194
Pf6N2E2_2195 - cI, at 2,602,302 to 2,602,958
_2195
Pf6N2E2_2196 - FIG00953347: hypothetical protein, at 2,603,081 to 2,603,422
_2196
Pf6N2E2_2197 - Predicted carboxypeptidase, at 2,603,922 to 2,605,067
_2197
Position (kb)
2602
2603 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2601.352 kb on + strand, within Pf6N2E2_2194 at 2601.353 kb on - strand, within Pf6N2E2_2194 at 2601.353 kb on - strand, within Pf6N2E2_2194 at 2601.406 kb on + strand, within Pf6N2E2_2194 at 2601.481 kb on + strand, within Pf6N2E2_2194 at 2601.482 kb on - strand, within Pf6N2E2_2194 at 2601.552 kb on + strand, within Pf6N2E2_2194 at 2601.552 kb on + strand, within Pf6N2E2_2194 at 2601.552 kb on + strand, within Pf6N2E2_2194 at 2601.552 kb on + strand, within Pf6N2E2_2194 at 2601.553 kb on - strand, within Pf6N2E2_2194 at 2601.553 kb on - strand, within Pf6N2E2_2194 at 2601.553 kb on - strand, within Pf6N2E2_2194 at 2601.611 kb on + strand, within Pf6N2E2_2194 at 2601.761 kb on + strand, within Pf6N2E2_2194 at 2601.761 kb on + strand, within Pf6N2E2_2194 at 2601.761 kb on + strand, within Pf6N2E2_2194 at 2601.762 kb on - strand, within Pf6N2E2_2194 at 2601.762 kb on - strand, within Pf6N2E2_2194 at 2601.762 kb on - strand, within Pf6N2E2_2194 at 2601.979 kb on + strand, within Pf6N2E2_2194 at 2601.979 kb on + strand, within Pf6N2E2_2194 at 2601.979 kb on + strand, within Pf6N2E2_2194 at 2601.980 kb on - strand, within Pf6N2E2_2194 at 2602.089 kb on + strand at 2602.090 kb on - strand at 2602.090 kb on - strand at 2602.213 kb on + strand at 2602.213 kb on + strand at 2602.214 kb on - strand at 2602.270 kb on + strand at 2602.270 kb on + strand at 2602.357 kb on + strand at 2602.357 kb on + strand at 2602.358 kb on - strand at 2602.397 kb on - strand, within Pf6N2E2_2195 at 2602.627 kb on + strand, within Pf6N2E2_2195 at 2602.628 kb on - strand, within Pf6N2E2_2195 at 2602.703 kb on - strand, within Pf6N2E2_2195 at 2602.936 kb on - strand at 2602.943 kb on + strand at 2602.944 kb on - strand at 2602.944 kb on - strand at 2602.961 kb on + strand at 2602.962 kb on - strand at 2602.962 kb on - strand at 2603.086 kb on + strand at 2603.086 kb on + strand at 2603.087 kb on - strand at 2603.175 kb on - strand, within Pf6N2E2_2196 at 2603.175 kb on - strand, within Pf6N2E2_2196 at 2603.175 kb on - strand, within Pf6N2E2_2196 at 2603.177 kb on + strand, within Pf6N2E2_2196 at 2603.177 kb on + strand, within Pf6N2E2_2196 at 2603.177 kb on + strand, within Pf6N2E2_2196 at 2603.178 kb on - strand, within Pf6N2E2_2196 at 2603.178 kb on - strand, within Pf6N2E2_2196 at 2603.517 kb on - strand at 2603.550 kb on + strand at 2603.565 kb on + strand at 2603.565 kb on + strand at 2603.565 kb on + strand at 2603.565 kb on + strand at 2603.565 kb on + strand at 2603.565 kb on + strand at 2603.566 kb on - strand at 2603.583 kb on + strand at 2603.583 kb on + strand at 2603.583 kb on + strand at 2603.583 kb on + strand at 2603.657 kb on - strand at 2603.713 kb on + strand at 2603.724 kb on + strand at 2603.764 kb on + strand at 2603.765 kb on - strand at 2603.803 kb on + strand at 2603.804 kb on - strand at 2603.804 kb on - strand at 2603.841 kb on + strand at 2603.842 kb on - strand at 2603.842 kb on - strand at 2603.842 kb on - strand at 2603.850 kb on + strand at 2603.850 kb on + strand at 2603.851 kb on - strand at 2603.851 kb on - strand at 2603.881 kb on + strand at 2603.881 kb on + strand at 2603.881 kb on + strand at 2603.881 kb on + strand at 2603.882 kb on - strand at 2603.882 kb on - strand at 2603.882 kb on - strand at 2603.886 kb on + strand at 2603.886 kb on + strand at 2603.887 kb on - strand at 2603.927 kb on + strand at 2603.943 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction DEANONOate 0.000625 M remove 2,601,352 + Pf6N2E2_2194 0.53 -1.2 2,601,353 - Pf6N2E2_2194 0.53 -0.0 2,601,353 - Pf6N2E2_2194 0.53 +0.9 2,601,406 + Pf6N2E2_2194 0.56 -1.0 2,601,481 + Pf6N2E2_2194 0.59 -2.5 2,601,482 - Pf6N2E2_2194 0.59 -0.9 2,601,552 + Pf6N2E2_2194 0.63 -0.7 2,601,552 + Pf6N2E2_2194 0.63 -0.7 2,601,552 + Pf6N2E2_2194 0.63 -0.6 2,601,552 + Pf6N2E2_2194 0.63 -2.3 2,601,553 - Pf6N2E2_2194 0.63 -1.3 2,601,553 - Pf6N2E2_2194 0.63 -3.1 2,601,553 - Pf6N2E2_2194 0.63 -0.9 2,601,611 + Pf6N2E2_2194 0.66 +0.5 2,601,761 + Pf6N2E2_2194 0.74 -1.0 2,601,761 + Pf6N2E2_2194 0.74 +0.7 2,601,761 + Pf6N2E2_2194 0.74 -0.7 2,601,762 - Pf6N2E2_2194 0.74 -1.6 2,601,762 - Pf6N2E2_2194 0.74 +0.4 2,601,762 - Pf6N2E2_2194 0.74 -1.4 2,601,979 + Pf6N2E2_2194 0.85 +1.5 2,601,979 + Pf6N2E2_2194 0.85 -2.7 2,601,979 + Pf6N2E2_2194 0.85 -0.9 2,601,980 - Pf6N2E2_2194 0.85 +0.0 2,602,089 + -0.2 2,602,090 - -1.5 2,602,090 - -2.2 2,602,213 + -0.7 2,602,213 + +0.0 2,602,214 - -1.8 2,602,270 + +0.9 2,602,270 + +0.1 2,602,357 + +1.6 2,602,357 + +0.8 2,602,358 - +1.2 2,602,397 - Pf6N2E2_2195 0.14 +1.4 2,602,627 + Pf6N2E2_2195 0.49 +2.1 2,602,628 - Pf6N2E2_2195 0.50 +0.5 2,602,703 - Pf6N2E2_2195 0.61 +0.5 2,602,936 - -2.6 2,602,943 + -1.0 2,602,944 - -3.6 2,602,944 - +0.9 2,602,961 + +1.2 2,602,962 - +0.2 2,602,962 - +1.2 2,603,086 + -1.5 2,603,086 + +2.0 2,603,087 - -0.3 2,603,175 - Pf6N2E2_2196 0.27 -2.1 2,603,175 - Pf6N2E2_2196 0.27 +0.4 2,603,175 - Pf6N2E2_2196 0.27 +0.7 2,603,177 + Pf6N2E2_2196 0.28 +0.9 2,603,177 + Pf6N2E2_2196 0.28 -1.5 2,603,177 + Pf6N2E2_2196 0.28 +0.3 2,603,178 - Pf6N2E2_2196 0.28 -0.3 2,603,178 - Pf6N2E2_2196 0.28 +0.0 2,603,517 - +0.7 2,603,550 + +0.8 2,603,565 + -0.1 2,603,565 + -0.8 2,603,565 + +0.9 2,603,565 + -0.9 2,603,565 + +0.2 2,603,565 + -0.0 2,603,566 - -0.9 2,603,583 + -1.9 2,603,583 + -0.4 2,603,583 + -1.7 2,603,583 + -0.4 2,603,657 - +0.4 2,603,713 + -0.8 2,603,724 + +1.1 2,603,764 + +0.7 2,603,765 - -0.5 2,603,803 + -0.2 2,603,804 - +0.1 2,603,804 - +0.8 2,603,841 + +0.3 2,603,842 - +0.7 2,603,842 - -1.5 2,603,842 - -2.8 2,603,850 + +0.4 2,603,850 + +0.0 2,603,851 - -2.4 2,603,851 - +1.2 2,603,881 + +0.4 2,603,881 + +0.7 2,603,881 + -0.0 2,603,881 + +0.9 2,603,882 - -0.3 2,603,882 - +0.7 2,603,882 - +1.5 2,603,886 + -0.6 2,603,886 + -1.2 2,603,887 - -0.5 2,603,927 + -1.9 2,603,943 - +0.3
Or see this region's nucleotide sequence