Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_478

Experiment: Cadmium chloride hemipentahydrate 0.000015625 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_477 and Pf6N2E2_478 are separated by 283 nucleotidesPf6N2E2_478 and Pf6N2E2_479 are separated by 3 nucleotides Pf6N2E2_477 - Branched-chain amino acid aminotransferase (EC 2.6.1.42), at 542,197 to 543,216 _477 Pf6N2E2_478 - Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (from data), at 543,500 to 544,885 _478 Pf6N2E2_479 - Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (EC 2.3.1.168) (from data), at 544,889 to 546,160 _479 Position (kb) 543 544 545Strain fitness (log2 ratio) -1 0 1at 542.652 kb on - strand, within Pf6N2E2_477at 542.747 kb on + strand, within Pf6N2E2_477at 542.843 kb on + strand, within Pf6N2E2_477at 543.173 kb on + strandat 543.174 kb on - strandat 543.174 kb on - strandat 543.226 kb on + strandat 543.226 kb on + strandat 543.227 kb on - strandat 543.227 kb on - strandat 543.288 kb on + strandat 543.288 kb on + strandat 543.288 kb on + strandat 543.288 kb on + strandat 543.288 kb on + strandat 543.288 kb on + strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.501 kb on + strandat 544.195 kb on - strand, within Pf6N2E2_478at 544.195 kb on - strand, within Pf6N2E2_478at 544.341 kb on + strand, within Pf6N2E2_478at 544.342 kb on - strand, within Pf6N2E2_478at 544.830 kb on + strandat 544.891 kb on - strandat 545.075 kb on + strand, within Pf6N2E2_479at 545.497 kb on - strand, within Pf6N2E2_479at 545.599 kb on - strand, within Pf6N2E2_479at 545.599 kb on - strand, within Pf6N2E2_479at 545.643 kb on + strand, within Pf6N2E2_479at 545.644 kb on - strand, within Pf6N2E2_479at 545.644 kb on - strand, within Pf6N2E2_479

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Per-strain Table

Position Strand Gene LocusTag Fraction Cadmium chloride hemipentahydrate 0.000015625 M
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542,652 - Pf6N2E2_477 0.45 +0.3
542,747 + Pf6N2E2_477 0.54 -0.0
542,843 + Pf6N2E2_477 0.63 -1.4
543,173 + -0.9
543,174 - -0.2
543,174 - -1.3
543,226 + -0.9
543,226 + +0.3
543,227 - +0.8
543,227 - -0.4
543,288 + -0.5
543,288 + -1.3
543,288 + -0.7
543,288 + -0.7
543,288 + +0.1
543,288 + -0.6
543,289 - +0.0
543,289 - -0.0
543,289 - +0.1
543,289 - -0.2
543,289 - +0.6
543,289 - -0.4
543,289 - -1.0
543,289 - +0.3
543,289 - +0.6
543,501 + +0.1
544,195 - Pf6N2E2_478 0.50 +1.3
544,195 - Pf6N2E2_478 0.50 +1.1
544,341 + Pf6N2E2_478 0.61 -0.4
544,342 - Pf6N2E2_478 0.61 +0.5
544,830 + +0.3
544,891 - -1.3
545,075 + Pf6N2E2_479 0.15 -0.6
545,497 - Pf6N2E2_479 0.48 -1.1
545,599 - Pf6N2E2_479 0.56 -0.8
545,599 - Pf6N2E2_479 0.56 +0.7
545,643 + Pf6N2E2_479 0.59 +1.1
545,644 - Pf6N2E2_479 0.59 -0.1
545,644 - Pf6N2E2_479 0.59 -0.2

Or see this region's nucleotide sequence