Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_462

Experiment: Cadmium chloride hemipentahydrate 0.000015625 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_461 and Pf6N2E2_462 are separated by 41 nucleotidesPf6N2E2_462 and Pf6N2E2_463 are separated by 167 nucleotidesPf6N2E2_463 and Pf6N2E2_464 are separated by 4 nucleotides Pf6N2E2_461 - Methyl-accepting chemotaxis protein, at 525,635 to 527,260 _461 Pf6N2E2_462 - Aspartate aminotransferase (EC 2.6.1.1), at 527,302 to 528,486 _462 Pf6N2E2_463 - Transcriptional regulatory protein RstA, at 528,654 to 529,355 _463 Pf6N2E2_464 - Sensory histidine kinase QseC, at 529,360 to 530,661 _464 Position (kb) 527 528 529Strain fitness (log2 ratio) -2 -1 0 1 2at 527.263 kb on + strandat 527.264 kb on - strandat 527.264 kb on - strandat 527.264 kb on - strandat 527.299 kb on + strandat 527.299 kb on + strandat 527.299 kb on + strandat 527.299 kb on + strandat 527.299 kb on + strandat 527.299 kb on + strandat 527.299 kb on + strandat 527.299 kb on + strandat 527.299 kb on + strandat 527.299 kb on + strandat 527.300 kb on - strandat 527.300 kb on - strandat 527.300 kb on - strandat 527.300 kb on - strandat 527.300 kb on - strandat 527.300 kb on - strandat 527.300 kb on - strandat 527.494 kb on + strand, within Pf6N2E2_462at 527.532 kb on + strand, within Pf6N2E2_462at 527.533 kb on - strand, within Pf6N2E2_462at 527.591 kb on + strand, within Pf6N2E2_462at 527.617 kb on + strand, within Pf6N2E2_462at 527.617 kb on + strand, within Pf6N2E2_462at 527.617 kb on + strand, within Pf6N2E2_462at 527.618 kb on - strand, within Pf6N2E2_462at 527.677 kb on + strand, within Pf6N2E2_462at 527.677 kb on + strand, within Pf6N2E2_462at 527.677 kb on + strand, within Pf6N2E2_462at 527.677 kb on + strand, within Pf6N2E2_462at 527.677 kb on + strand, within Pf6N2E2_462at 527.677 kb on + strand, within Pf6N2E2_462at 527.677 kb on + strand, within Pf6N2E2_462at 527.677 kb on + strand, within Pf6N2E2_462at 527.678 kb on - strand, within Pf6N2E2_462at 527.678 kb on - strand, within Pf6N2E2_462at 527.678 kb on - strand, within Pf6N2E2_462at 527.678 kb on - strand, within Pf6N2E2_462at 527.678 kb on - strand, within Pf6N2E2_462at 527.678 kb on - strand, within Pf6N2E2_462at 527.678 kb on - strand, within Pf6N2E2_462at 527.678 kb on - strand, within Pf6N2E2_462at 527.678 kb on - strand, within Pf6N2E2_462at 527.686 kb on + strand, within Pf6N2E2_462at 527.686 kb on + strand, within Pf6N2E2_462at 527.686 kb on + strand, within Pf6N2E2_462at 527.686 kb on + strand, within Pf6N2E2_462at 527.686 kb on + strand, within Pf6N2E2_462at 527.686 kb on + strand, within Pf6N2E2_462at 527.686 kb on + strand, within Pf6N2E2_462at 527.687 kb on - strand, within Pf6N2E2_462at 527.687 kb on - strand, within Pf6N2E2_462at 527.687 kb on - strand, within Pf6N2E2_462at 527.687 kb on - strand, within Pf6N2E2_462at 527.687 kb on - strand, within Pf6N2E2_462at 527.687 kb on - strand, within Pf6N2E2_462at 527.687 kb on - strand, within Pf6N2E2_462at 527.687 kb on - strand, within Pf6N2E2_462at 527.921 kb on - strand, within Pf6N2E2_462at 527.935 kb on + strand, within Pf6N2E2_462at 527.936 kb on - strand, within Pf6N2E2_462at 527.936 kb on - strand, within Pf6N2E2_462at 528.096 kb on + strand, within Pf6N2E2_462at 528.096 kb on + strand, within Pf6N2E2_462at 528.096 kb on + strand, within Pf6N2E2_462at 528.096 kb on + strand, within Pf6N2E2_462at 528.096 kb on + strand, within Pf6N2E2_462at 528.097 kb on - strand, within Pf6N2E2_462at 528.097 kb on - strand, within Pf6N2E2_462at 528.097 kb on - strand, within Pf6N2E2_462at 528.097 kb on - strand, within Pf6N2E2_462at 528.097 kb on - strand, within Pf6N2E2_462at 528.097 kb on - strand, within Pf6N2E2_462at 528.097 kb on - strand, within Pf6N2E2_462at 528.188 kb on - strand, within Pf6N2E2_462at 528.337 kb on + strand, within Pf6N2E2_462at 528.338 kb on - strand, within Pf6N2E2_462at 528.391 kb on - strandat 528.546 kb on + strandat 528.546 kb on + strandat 528.546 kb on + strandat 528.546 kb on + strandat 528.546 kb on + strandat 528.548 kb on + strandat 528.711 kb on + strandat 528.712 kb on - strandat 528.948 kb on + strand, within Pf6N2E2_463at 528.948 kb on + strand, within Pf6N2E2_463at 528.949 kb on - strand, within Pf6N2E2_463at 528.949 kb on - strand, within Pf6N2E2_463at 528.949 kb on - strand, within Pf6N2E2_463at 528.949 kb on - strand, within Pf6N2E2_463at 528.949 kb on - strand, within Pf6N2E2_463at 528.995 kb on + strand, within Pf6N2E2_463at 529.131 kb on + strand, within Pf6N2E2_463at 529.132 kb on - strand, within Pf6N2E2_463at 529.132 kb on - strand, within Pf6N2E2_463at 529.171 kb on + strand, within Pf6N2E2_463at 529.171 kb on + strand, within Pf6N2E2_463at 529.172 kb on - strand, within Pf6N2E2_463at 529.172 kb on - strand, within Pf6N2E2_463at 529.172 kb on - strand, within Pf6N2E2_463at 529.406 kb on - strandat 529.420 kb on + strandat 529.421 kb on - strandat 529.471 kb on + strandat 529.471 kb on + strandat 529.471 kb on + strandat 529.471 kb on + strandat 529.471 kb on + strandat 529.471 kb on + strandat 529.472 kb on - strandat 529.484 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cadmium chloride hemipentahydrate 0.000015625 M
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527,263 + +0.5
527,264 - +0.3
527,264 - +1.3
527,264 - -0.4
527,299 + +0.2
527,299 + +0.0
527,299 + -0.2
527,299 + +0.2
527,299 + -0.1
527,299 + -1.1
527,299 + -0.5
527,299 + +0.2
527,299 + +0.8
527,299 + -0.7
527,300 - +2.0
527,300 - -1.7
527,300 - +0.7
527,300 - -1.0
527,300 - -0.2
527,300 - +0.8
527,300 - -0.0
527,494 + Pf6N2E2_462 0.16 -0.5
527,532 + Pf6N2E2_462 0.19 +1.6
527,533 - Pf6N2E2_462 0.19 +0.1
527,591 + Pf6N2E2_462 0.24 -0.2
527,617 + Pf6N2E2_462 0.27 +0.9
527,617 + Pf6N2E2_462 0.27 -0.0
527,617 + Pf6N2E2_462 0.27 +0.8
527,618 - Pf6N2E2_462 0.27 +0.5
527,677 + Pf6N2E2_462 0.32 +0.1
527,677 + Pf6N2E2_462 0.32 +0.4
527,677 + Pf6N2E2_462 0.32 -0.0
527,677 + Pf6N2E2_462 0.32 +0.0
527,677 + Pf6N2E2_462 0.32 +0.1
527,677 + Pf6N2E2_462 0.32 +1.2
527,677 + Pf6N2E2_462 0.32 +0.1
527,677 + Pf6N2E2_462 0.32 +0.2
527,678 - Pf6N2E2_462 0.32 +0.4
527,678 - Pf6N2E2_462 0.32 +1.0
527,678 - Pf6N2E2_462 0.32 -0.1
527,678 - Pf6N2E2_462 0.32 +0.7
527,678 - Pf6N2E2_462 0.32 +0.5
527,678 - Pf6N2E2_462 0.32 +0.5
527,678 - Pf6N2E2_462 0.32 +0.6
527,678 - Pf6N2E2_462 0.32 -0.5
527,678 - Pf6N2E2_462 0.32 -0.2
527,686 + Pf6N2E2_462 0.32 -0.4
527,686 + Pf6N2E2_462 0.32 +0.7
527,686 + Pf6N2E2_462 0.32 -1.8
527,686 + Pf6N2E2_462 0.32 -0.3
527,686 + Pf6N2E2_462 0.32 -1.1
527,686 + Pf6N2E2_462 0.32 +0.2
527,686 + Pf6N2E2_462 0.32 +0.4
527,687 - Pf6N2E2_462 0.32 +0.6
527,687 - Pf6N2E2_462 0.32 -0.6
527,687 - Pf6N2E2_462 0.32 +0.5
527,687 - Pf6N2E2_462 0.32 +0.4
527,687 - Pf6N2E2_462 0.32 -0.9
527,687 - Pf6N2E2_462 0.32 -0.4
527,687 - Pf6N2E2_462 0.32 +0.4
527,687 - Pf6N2E2_462 0.32 +0.7
527,921 - Pf6N2E2_462 0.52 -0.5
527,935 + Pf6N2E2_462 0.53 +1.0
527,936 - Pf6N2E2_462 0.54 +0.1
527,936 - Pf6N2E2_462 0.54 +1.1
528,096 + Pf6N2E2_462 0.67 -0.1
528,096 + Pf6N2E2_462 0.67 -0.4
528,096 + Pf6N2E2_462 0.67 -0.2
528,096 + Pf6N2E2_462 0.67 -0.3
528,096 + Pf6N2E2_462 0.67 +0.2
528,097 - Pf6N2E2_462 0.67 -0.7
528,097 - Pf6N2E2_462 0.67 -0.1
528,097 - Pf6N2E2_462 0.67 +0.9
528,097 - Pf6N2E2_462 0.67 +0.2
528,097 - Pf6N2E2_462 0.67 -1.0
528,097 - Pf6N2E2_462 0.67 +0.2
528,097 - Pf6N2E2_462 0.67 +0.1
528,188 - Pf6N2E2_462 0.75 -0.1
528,337 + Pf6N2E2_462 0.87 +0.3
528,338 - Pf6N2E2_462 0.87 -0.2
528,391 - +0.9
528,546 + +0.2
528,546 + +0.2
528,546 + +1.1
528,546 + +1.2
528,546 + +0.6
528,548 + +0.7
528,711 + +1.1
528,712 - +0.4
528,948 + Pf6N2E2_463 0.42 -0.0
528,948 + Pf6N2E2_463 0.42 +0.3
528,949 - Pf6N2E2_463 0.42 +0.3
528,949 - Pf6N2E2_463 0.42 +0.9
528,949 - Pf6N2E2_463 0.42 -0.6
528,949 - Pf6N2E2_463 0.42 -1.4
528,949 - Pf6N2E2_463 0.42 +0.3
528,995 + Pf6N2E2_463 0.49 +0.3
529,131 + Pf6N2E2_463 0.68 +1.1
529,132 - Pf6N2E2_463 0.68 +0.2
529,132 - Pf6N2E2_463 0.68 -0.1
529,171 + Pf6N2E2_463 0.74 +1.3
529,171 + Pf6N2E2_463 0.74 +0.2
529,172 - Pf6N2E2_463 0.74 +1.5
529,172 - Pf6N2E2_463 0.74 +0.7
529,172 - Pf6N2E2_463 0.74 -1.2
529,406 - -0.7
529,420 + +0.4
529,421 - +0.6
529,471 + +0.3
529,471 + +2.0
529,471 + +1.7
529,471 + +0.7
529,471 + -0.4
529,471 + -1.5
529,472 - +1.2
529,484 - -1.2

Or see this region's nucleotide sequence