Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_401

Experiment: Cadmium chloride hemipentahydrate 0.000015625 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_400 and Pf6N2E2_401 are separated by 135 nucleotidesPf6N2E2_401 and Pf6N2E2_402 are separated by 97 nucleotidesPf6N2E2_402 and Pf6N2E2_403 are separated by 39 nucleotides Pf6N2E2_400 - Ferrichrome-iron receptor, at 468,765 to 471,185 _400 Pf6N2E2_401 - Cyn operon transcriptional activator, at 471,321 to 472,220 _401 Pf6N2E2_402 - Carbonic anhydrase (EC 4.2.1.1), at 472,318 to 472,977 _402 Pf6N2E2_403 - Cyanate hydratase (EC 4.2.1.104), at 473,017 to 473,487 _403 Position (kb) 471 472 473Strain fitness (log2 ratio) -2 -1 0 1 2at 470.460 kb on + strand, within Pf6N2E2_400at 470.461 kb on - strand, within Pf6N2E2_400at 470.461 kb on - strand, within Pf6N2E2_400at 470.487 kb on + strand, within Pf6N2E2_400at 470.514 kb on + strand, within Pf6N2E2_400at 470.515 kb on - strand, within Pf6N2E2_400at 470.527 kb on - strand, within Pf6N2E2_400at 470.630 kb on - strand, within Pf6N2E2_400at 470.719 kb on - strand, within Pf6N2E2_400at 470.719 kb on - strand, within Pf6N2E2_400at 470.721 kb on + strand, within Pf6N2E2_400at 470.994 kb on + strandat 470.995 kb on - strandat 471.105 kb on + strandat 471.105 kb on + strandat 471.105 kb on + strandat 471.105 kb on + strandat 471.106 kb on - strandat 471.106 kb on - strandat 471.106 kb on - strandat 471.129 kb on + strandat 471.152 kb on + strandat 471.170 kb on + strandat 471.233 kb on + strandat 471.233 kb on + strandat 471.234 kb on - strandat 471.234 kb on - strandat 471.248 kb on + strandat 471.249 kb on - strandat 471.314 kb on - strandat 471.322 kb on + strandat 471.322 kb on + strandat 471.322 kb on + strandat 471.323 kb on - strandat 471.323 kb on - strandat 471.323 kb on - strandat 471.492 kb on + strand, within Pf6N2E2_401at 471.493 kb on - strand, within Pf6N2E2_401at 471.493 kb on - strand, within Pf6N2E2_401at 471.501 kb on + strand, within Pf6N2E2_401at 471.502 kb on - strand, within Pf6N2E2_401at 471.590 kb on - strand, within Pf6N2E2_401at 471.590 kb on - strand, within Pf6N2E2_401at 471.755 kb on + strand, within Pf6N2E2_401at 471.756 kb on - strand, within Pf6N2E2_401at 471.759 kb on + strand, within Pf6N2E2_401at 471.854 kb on + strand, within Pf6N2E2_401at 471.855 kb on - strand, within Pf6N2E2_401at 471.898 kb on + strand, within Pf6N2E2_401at 471.898 kb on + strand, within Pf6N2E2_401at 471.898 kb on + strand, within Pf6N2E2_401at 471.899 kb on - strand, within Pf6N2E2_401at 471.899 kb on - strand, within Pf6N2E2_401at 471.994 kb on + strand, within Pf6N2E2_401at 472.009 kb on + strand, within Pf6N2E2_401at 472.018 kb on + strand, within Pf6N2E2_401at 472.018 kb on + strand, within Pf6N2E2_401at 472.018 kb on + strand, within Pf6N2E2_401at 472.019 kb on - strand, within Pf6N2E2_401at 472.019 kb on - strand, within Pf6N2E2_401at 472.019 kb on - strand, within Pf6N2E2_401at 472.186 kb on + strandat 472.187 kb on - strandat 472.187 kb on - strandat 472.279 kb on + strandat 472.279 kb on + strandat 472.279 kb on + strandat 472.279 kb on + strandat 472.280 kb on - strandat 472.280 kb on - strandat 472.280 kb on - strandat 472.601 kb on + strand, within Pf6N2E2_402at 472.602 kb on - strand, within Pf6N2E2_402at 472.751 kb on + strand, within Pf6N2E2_402at 472.861 kb on + strand, within Pf6N2E2_402at 472.861 kb on + strand, within Pf6N2E2_402at 472.862 kb on - strand, within Pf6N2E2_402at 472.862 kb on - strand, within Pf6N2E2_402at 472.862 kb on - strand, within Pf6N2E2_402at 472.862 kb on - strand, within Pf6N2E2_402at 472.862 kb on - strand, within Pf6N2E2_402at 472.985 kb on - strandat 473.012 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cadmium chloride hemipentahydrate 0.000015625 M
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470,460 + Pf6N2E2_400 0.70 +0.2
470,461 - Pf6N2E2_400 0.70 -0.2
470,461 - Pf6N2E2_400 0.70 +0.4
470,487 + Pf6N2E2_400 0.71 +0.4
470,514 + Pf6N2E2_400 0.72 +0.4
470,515 - Pf6N2E2_400 0.72 +0.8
470,527 - Pf6N2E2_400 0.73 -0.9
470,630 - Pf6N2E2_400 0.77 -0.6
470,719 - Pf6N2E2_400 0.81 +1.1
470,719 - Pf6N2E2_400 0.81 +0.8
470,721 + Pf6N2E2_400 0.81 -0.4
470,994 + +0.7
470,995 - -0.2
471,105 + -1.4
471,105 + -0.1
471,105 + -0.4
471,105 + -0.5
471,106 - +0.8
471,106 - +0.4
471,106 - -1.3
471,129 + +0.1
471,152 + -0.4
471,170 + -0.7
471,233 + +1.7
471,233 + +1.1
471,234 - +1.1
471,234 - -1.6
471,248 + -0.8
471,249 - -0.6
471,314 - +0.1
471,322 + -0.1
471,322 + -1.3
471,322 + +0.7
471,323 - +0.2
471,323 - +0.4
471,323 - -1.3
471,492 + Pf6N2E2_401 0.19 +0.3
471,493 - Pf6N2E2_401 0.19 -0.7
471,493 - Pf6N2E2_401 0.19 -0.2
471,501 + Pf6N2E2_401 0.20 -0.1
471,502 - Pf6N2E2_401 0.20 +0.4
471,590 - Pf6N2E2_401 0.30 +0.2
471,590 - Pf6N2E2_401 0.30 +0.8
471,755 + Pf6N2E2_401 0.48 +0.0
471,756 - Pf6N2E2_401 0.48 -0.1
471,759 + Pf6N2E2_401 0.49 -0.1
471,854 + Pf6N2E2_401 0.59 -0.8
471,855 - Pf6N2E2_401 0.59 -0.5
471,898 + Pf6N2E2_401 0.64 -0.1
471,898 + Pf6N2E2_401 0.64 -0.6
471,898 + Pf6N2E2_401 0.64 -0.1
471,899 - Pf6N2E2_401 0.64 +1.8
471,899 - Pf6N2E2_401 0.64 +0.8
471,994 + Pf6N2E2_401 0.75 +0.8
472,009 + Pf6N2E2_401 0.76 -0.4
472,018 + Pf6N2E2_401 0.77 +0.7
472,018 + Pf6N2E2_401 0.77 -0.1
472,018 + Pf6N2E2_401 0.77 -0.8
472,019 - Pf6N2E2_401 0.78 -0.6
472,019 - Pf6N2E2_401 0.78 +1.5
472,019 - Pf6N2E2_401 0.78 -0.0
472,186 + +0.7
472,187 - -0.3
472,187 - -0.5
472,279 + -0.7
472,279 + -0.5
472,279 + +1.4
472,279 + -0.0
472,280 - -0.3
472,280 - -0.9
472,280 - +1.0
472,601 + Pf6N2E2_402 0.43 -0.1
472,602 - Pf6N2E2_402 0.43 -0.2
472,751 + Pf6N2E2_402 0.66 -0.1
472,861 + Pf6N2E2_402 0.82 +0.6
472,861 + Pf6N2E2_402 0.82 -0.4
472,862 - Pf6N2E2_402 0.82 +1.4
472,862 - Pf6N2E2_402 0.82 +0.2
472,862 - Pf6N2E2_402 0.82 +0.2
472,862 - Pf6N2E2_402 0.82 -2.3
472,862 - Pf6N2E2_402 0.82 -1.1
472,985 - -1.6
473,012 - -0.7

Or see this region's nucleotide sequence