Experiment: ShewMM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3553 and purE are separated by 262 nucleotides purE and purK overlap by 1 nucleotides purK and SO3556 are separated by 148 nucleotides
SO3553: SO3553 - sulfate permease family protein (NCBI ptt file), at 3,710,411 to 3,712,177
SO3553
SO3554: purE - phosphoribosylaminoimidazole carboxylase, catalytic subunit (NCBI ptt file), at 3,712,440 to 3,712,931
purE
SO3555: purK - phosphoribosylaminoimidazole carboxylase, ATPase subunit (NCBI ptt file), at 3,712,931 to 3,714,028
purK
SO3556: SO3556 - cyclic nucleotide phosphodiesterase, putative (NCBI ptt file), at 3,714,177 to 3,715,439
SO3556
Position (kb)
3712
3713
3714
3715 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3712.147 kb on + strand at 3712.262 kb on + strand at 3712.262 kb on + strand at 3712.314 kb on + strand at 3712.339 kb on - strand at 3712.353 kb on + strand at 3712.370 kb on - strand at 3712.378 kb on + strand at 3712.461 kb on + strand at 3712.535 kb on - strand, within purE at 3712.537 kb on + strand, within purE at 3712.629 kb on + strand, within purE at 3712.637 kb on - strand, within purE at 3712.647 kb on + strand, within purE at 3712.709 kb on - strand, within purE at 3712.986 kb on + strand at 3712.989 kb on - strand at 3712.991 kb on + strand at 3712.991 kb on + strand at 3712.999 kb on - strand at 3712.999 kb on - strand at 3713.137 kb on + strand, within purK at 3713.137 kb on + strand, within purK at 3713.145 kb on - strand, within purK at 3713.171 kb on + strand, within purK at 3713.227 kb on + strand, within purK at 3713.229 kb on - strand, within purK at 3713.277 kb on - strand, within purK at 3713.342 kb on + strand, within purK at 3713.350 kb on - strand, within purK at 3713.352 kb on + strand, within purK at 3713.469 kb on + strand, within purK at 3713.501 kb on - strand, within purK at 3713.584 kb on + strand, within purK at 3713.752 kb on + strand, within purK at 3713.785 kb on - strand, within purK at 3713.843 kb on - strand, within purK at 3713.848 kb on - strand, within purK at 3713.850 kb on + strand, within purK at 3713.933 kb on + strand at 3714.062 kb on + strand at 3714.063 kb on - strand at 3714.101 kb on - strand at 3714.104 kb on + strand at 3714.122 kb on - strand at 3714.297 kb on + strand at 3714.414 kb on - strand, within SO3556 at 3714.417 kb on + strand, within SO3556 at 3714.511 kb on - strand, within SO3556 at 3714.517 kb on + strand, within SO3556 at 3714.621 kb on + strand, within SO3556 at 3714.676 kb on - strand, within SO3556 at 3714.758 kb on - strand, within SO3556 at 3714.789 kb on - strand, within SO3556 at 3714.791 kb on + strand, within SO3556 at 3714.791 kb on + strand, within SO3556 at 3714.874 kb on + strand, within SO3556 at 3714.900 kb on + strand, within SO3556
Per-strain Table
Position Strand Gene LocusTag Fraction ShewMM remove 3,712,147 + -0.7 3,712,262 + -0.4 3,712,262 + -0.9 3,712,314 + -0.3 3,712,339 - -4.9 3,712,353 + +1.1 3,712,370 - -5.5 3,712,378 + +0.6 3,712,461 + -2.1 3,712,535 - purE SO3554 0.19 -4.7 3,712,537 + purE SO3554 0.20 -4.0 3,712,629 + purE SO3554 0.38 -2.4 3,712,637 - purE SO3554 0.40 -3.5 3,712,647 + purE SO3554 0.42 -4.2 3,712,709 - purE SO3554 0.55 -4.5 3,712,986 + -2.9 3,712,989 - -4.0 3,712,991 + -4.2 3,712,991 + -2.1 3,712,999 - -3.2 3,712,999 - -3.5 3,713,137 + purK SO3555 0.19 -3.0 3,713,137 + purK SO3555 0.19 -3.4 3,713,145 - purK SO3555 0.19 -3.1 3,713,171 + purK SO3555 0.22 -3.8 3,713,227 + purK SO3555 0.27 -2.2 3,713,229 - purK SO3555 0.27 -2.2 3,713,277 - purK SO3555 0.32 -4.9 3,713,342 + purK SO3555 0.37 -3.9 3,713,350 - purK SO3555 0.38 -3.2 3,713,352 + purK SO3555 0.38 -3.7 3,713,469 + purK SO3555 0.49 -3.3 3,713,501 - purK SO3555 0.52 -4.3 3,713,584 + purK SO3555 0.59 -2.6 3,713,752 + purK SO3555 0.75 -0.8 3,713,785 - purK SO3555 0.78 -3.5 3,713,843 - purK SO3555 0.83 -4.8 3,713,848 - purK SO3555 0.84 -3.0 3,713,850 + purK SO3555 0.84 -5.6 3,713,933 + -3.4 3,714,062 + +1.0 3,714,063 - +0.3 3,714,101 - +0.1 3,714,104 + -0.4 3,714,122 - -0.4 3,714,297 + +0.0 3,714,414 - SO3556 0.19 -0.6 3,714,417 + SO3556 0.19 -0.4 3,714,511 - SO3556 0.26 -0.1 3,714,517 + SO3556 0.27 +0.1 3,714,621 + SO3556 0.35 +0.4 3,714,676 - SO3556 0.40 +0.6 3,714,758 - SO3556 0.46 -0.5 3,714,789 - SO3556 0.48 +0.2 3,714,791 + SO3556 0.49 -0.5 3,714,791 + SO3556 0.49 +1.1 3,714,874 + SO3556 0.55 -0.3 3,714,900 + SO3556 0.57 +0.6
Or see this region's nucleotide sequence