Experiment: ShewMM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2573 and SO2575 are separated by 394 nucleotides SO2575 and minC are separated by 123 nucleotides minC and minD are separated by 27 nucleotides
SO2573: SO2573 - conserved hypothetical protein (NCBI ptt file), at 2,703,468 to 2,703,908
SO2573
SO2575: SO2575 - conserved hypothetical protein (NCBI ptt file), at 2,704,303 to 2,704,581
SO2575
SO2576: minC - septum site-determining protein MinC (NCBI ptt file), at 2,704,705 to 2,705,370
minC
SO2577: minD - septum site-determining protein MinD (NCBI ptt file), at 2,705,398 to 2,706,207
minD
Position (kb)
2704
2705 Strain fitness (log2 ratio)
-1
0
1
2 at 2703.309 kb on - strand at 2703.311 kb on + strand at 2703.316 kb on + strand at 2703.319 kb on - strand at 2703.319 kb on - strand at 2703.515 kb on + strand, within SO2573 at 2703.515 kb on + strand, within SO2573 at 2703.515 kb on + strand, within SO2573 at 2703.515 kb on + strand, within SO2573 at 2703.621 kb on + strand, within SO2573 at 2703.707 kb on - strand, within SO2573 at 2703.792 kb on - strand, within SO2573 at 2703.871 kb on - strand at 2703.973 kb on + strand at 2704.189 kb on + strand at 2704.197 kb on - strand at 2704.197 kb on - strand at 2704.197 kb on - strand at 2704.253 kb on + strand at 2704.253 kb on + strand at 2704.397 kb on + strand, within SO2575 at 2704.405 kb on - strand, within SO2575 at 2704.405 kb on - strand, within SO2575 at 2704.514 kb on - strand, within SO2575 at 2704.541 kb on + strand, within SO2575 at 2704.578 kb on + strand at 2704.609 kb on - strand at 2704.628 kb on + strand at 2704.630 kb on + strand at 2704.630 kb on + strand at 2704.636 kb on - strand at 2704.638 kb on - strand at 2704.638 kb on - strand at 2704.692 kb on + strand at 2704.707 kb on + strand at 2704.713 kb on + strand at 2704.936 kb on + strand, within minC at 2704.972 kb on + strand, within minC at 2704.978 kb on + strand, within minC at 2704.979 kb on + strand, within minC at 2704.984 kb on + strand, within minC at 2704.986 kb on - strand, within minC at 2704.992 kb on - strand, within minC at 2705.082 kb on + strand, within minC at 2705.271 kb on + strand, within minC at 2705.271 kb on + strand, within minC at 2705.279 kb on - strand, within minC at 2705.311 kb on - strand at 2705.384 kb on + strand at 2705.446 kb on + strand at 2705.468 kb on + strand at 2705.531 kb on + strand, within minD at 2705.566 kb on + strand, within minD at 2705.574 kb on - strand, within minD at 2705.574 kb on - strand, within minD
Per-strain Table
Position Strand Gene LocusTag Fraction ShewMM remove 2,703,309 - +1.2 2,703,311 + -0.0 2,703,316 + -1.0 2,703,319 - -0.3 2,703,319 - -1.5 2,703,515 + SO2573 0.11 -0.0 2,703,515 + SO2573 0.11 +0.2 2,703,515 + SO2573 0.11 -0.2 2,703,515 + SO2573 0.11 -1.5 2,703,621 + SO2573 0.35 -0.1 2,703,707 - SO2573 0.54 -0.5 2,703,792 - SO2573 0.73 -0.5 2,703,871 - -0.7 2,703,973 + +0.1 2,704,189 + -0.3 2,704,197 - -0.0 2,704,197 - +1.7 2,704,197 - +0.0 2,704,253 + +1.5 2,704,253 + +0.9 2,704,397 + SO2575 0.34 +0.5 2,704,405 - SO2575 0.37 +1.0 2,704,405 - SO2575 0.37 +0.4 2,704,514 - SO2575 0.76 +0.2 2,704,541 + SO2575 0.85 +1.9 2,704,578 + +0.0 2,704,609 - +1.1 2,704,628 + -0.0 2,704,630 + +0.6 2,704,630 + -0.3 2,704,636 - +1.1 2,704,638 - -0.7 2,704,638 - +0.3 2,704,692 + -0.3 2,704,707 + +0.1 2,704,713 + +0.7 2,704,936 + minC SO2576 0.35 -0.7 2,704,972 + minC SO2576 0.40 -0.0 2,704,978 + minC SO2576 0.41 -0.7 2,704,979 + minC SO2576 0.41 +0.6 2,704,984 + minC SO2576 0.42 +0.3 2,704,986 - minC SO2576 0.42 +0.5 2,704,992 - minC SO2576 0.43 -0.9 2,705,082 + minC SO2576 0.57 -0.5 2,705,271 + minC SO2576 0.85 +0.0 2,705,271 + minC SO2576 0.85 -0.2 2,705,279 - minC SO2576 0.86 +0.4 2,705,311 - +0.6 2,705,384 + +0.2 2,705,446 + +0.9 2,705,468 + +0.6 2,705,531 + minD SO2577 0.16 +1.1 2,705,566 + minD SO2577 0.21 +0.9 2,705,574 - minD SO2577 0.22 +0.4 2,705,574 - minD SO2577 0.22 +0.4
Or see this region's nucleotide sequence